In vitro exposure with β-hydroxy-β-methylbutyrate enhances chicken macrophage growth and function

Peterson, AL; Qureshi, M. A.; Ferket, P. R.; Fuller, John C.

doi:10.1016/s0165-2427(98)00211-6

Cited by 53 publications

(59 citation statements)

References 27 publications

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“…Oral administration of KIC and HMB to steers increased DNA synthesis by mitogen-stimulated lymphocytes in animals [13,15]. The in vitro study showed that HMB has a stimulating effect on the macrophage activity of chickens; it induces proliferation of macrophages in culture as well as enhances Specific ASC per 10 6 leucocytes macrophage effector functions such as nitrite production and phagocytosis [16].…”

Section: Resultsmentioning

confidence: 95%

“…Leucine is found in all dietary proteins and is an essential building block of protein in all tissues. Scientists have proposed that under stressful situations both animals and humans cannot eat or synthesize enough HMB in the body to meet the needs of the tissues [9][10][11][12][13][14][15][16]. The hypothesis that HMB could be an effective supplement in maximizing muscle growth and preventing muscle loss during stressful situations was first tested in animals.…”

Section: Introductionmentioning

confidence: 99%

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Influence of HMB (?-hydroxy-?-methylbutyrate) on antibody secreting cells (ASC) after in vitro and in vivo immunization with the anti-Yersinia ruckeri vaccine of rainbow trout (Oncorhynchus mykiss)

Siwicki¹,

Morand²,

Fuller³

et al. 2001

Vet. Res.

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-In practice, protection of fish against disease by immunization is of limited effectiveness. Therefore, research is concentrated on how to improve the potency and efficacy of vaccines and how to optimally activate the cell-mediated immunity and the specific antibody response. In the present study, the influence of HMB (β-Hydroxy-β-methylbutyrate) on the antibody secreting cells (ASC) after both in vitro and in vivo immunization of rainbow trout (Oncorhynchus mykiss) with the anti-Yersiniose vaccine was studied. For in vitro immunization, the spleens from 160 fish were sampled and placed each in 35 mm sterile wells with medium containing HMB at concentrations of 0, 0.1, 1, 5, 10, 25, 50 or 100 µg/mL of medium. The spleens from 80 fish were injected with the vaccine and incubated at 14°C for 10 days. For the in vivo study, fish were fed pellets containing HMB at doses of 0, 10, 25 and 50 mg/kg bw per day. After 2 weeks of HMB supplementation, the fish were immunized by intraperitoneal injection of the vaccine. At 7, 14, 18, 21, 28 and 35 days after immunization, pronephros were taken from 10 fish in each group for testing. When analyzed by the ELISPOT assay, HMB increased the number of splenic ASC after in vitro immunization at concentrations between 10 and 100 µg/mL (P < 0.05). Dietary HMB also increased the number of total and specific ASC when the fish were vaccinated in vivo. In conclusion, the results of the present study showed that HMB increases the levels of specific ASC after both in vitro and in vivo immunization of rainbow trout with the anti-Yersinia ruckeri vaccine.HMB / rainbow trout / anti-Yersiniose vaccine / antibody secreting cells Vet. Res. 32 (2001) [491][492][493][494][495][496][497][498] 491

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Influence of HMB (?-hydroxy-?-methylbutyrate) on antibody secreting cells (ASC) after in vitro and in vivo immunization with the anti-Yersinia ruckeri vaccine of rainbow trout (Oncorhynchus mykiss)

Siwicki¹,

Morand²,

Fuller³

et al. 2001

Vet. Res.

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“…In comparison with control, the proliferative activity of B lymphocytes in experimental calves increased significantly by 64%, 47% and 39%, and the activity T lymphocytes -by 104%, 41% and 25% on days 15, 30 and 60, respectively (Table 1). In an in vitro study investigating the responses of the chicken macrophage cell line (MQ-NCSU) to HMB (Peterson et al 1999), the expression of Fc receptors (FcR) on the surface of macrophages was intensified and significantly higher in comparison with control. HMB was also found to induce macrophage proliferation in cell lines and intensify their effector (cytotoxic) functions, such as nitrite production and the phagocytic activity of macrophages.…”

Section: Resultsmentioning

confidence: 99%

“…An example of a immune stimulator is β-hydroxy-β-methylbutyric acid (HMB) which Correspondence to: R. Wójcik, e-mail: brandy@uwm.edu.pl occurs naturally in humans, animals and plants. The results of research investigating various animal species (fish, birds, mammals), including limited studies of cattle (single experiments performed on older animals) have demonstrated that HMB plays an important role in stimulating the immune system and preventing disease (Talleyrand et al 1994, Peterson et al 1999, Puchajda-Skowrońska et al 2006. The objective of this study was to evaluate the effect of in-feed Key: * p < 0.05; ** p < 0.01; *** p < 0.001; SD -standard deviation; A -p ≤ 0.05; B -p ≤ 0.01; C -p ≤ 0.001 in comparison to day 0.…”

Section: Introductionmentioning

confidence: 99%

The effect of β-hydroxy-β-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves

Wójcik¹,

Małaczewska²,

Siwicki³

et al. 2013

Polish Journal of Veterinary Sciences

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The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS-and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.

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“…many animal species (Gatnau et al 1995, Peterson et al 1999, Puchajda-Skowrońska et al 2006, but very few studies investigating its beneficial effects have been conducted on cattle (Talleyrand et al 1994, Wójcik et al 2013. The objective of this study was to evaluate the effect of HMB supplementation on selected parameters of the humoral immunity in calves.…”

Section: Introductionmentioning

confidence: 99%

The effect of β-hydroxy-β-methylbutyrate (HMB) on selected parameters of humoral immunity in calves

Wójcik¹,

Małaczewska²,

Siwicki³

et al. 2014

Polish Journal of Veterinary Sciences

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The objective of this study was to evaluate the effect of HMB on selected parameters of the humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control (group K) and experimental (group H) animals. The feed administered to the experimental calves was supplemented with HMB at 40 mg/kg BW, whereas the control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following immunological parameters: total protein levels, gammaglobulin levels, lysozyme activity and ceruloplasmin activity. An analysis of the results obtained revealed a significant increase (p < 0.05; p < 0.01; p < 0.001 respectively) in gammaglobulin levels and lysozyme activity throughout the entire experimental period, an increase (p < 0.05; p < 0.01 respectively) in ceruloplasmin activity on experimental days 15 and 30, but no changes in serum total protein levels of calves administered HMB as compared to those found in the control group.

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In vitro exposure with β-hydroxy-β-methylbutyrate enhances chicken macrophage growth and function

Cited by 53 publications

References 27 publications

Influence of HMB (?-hydroxy-?-methylbutyrate) on antibody secreting cells (ASC) after in vitro and in vivo immunization with the anti-Yersinia ruckeri vaccine of rainbow trout (Oncorhynchus mykiss)

Influence of HMB (?-hydroxy-?-methylbutyrate) on antibody secreting cells (ASC) after in vitro and in vivo immunization with the anti-Yersinia ruckeri vaccine of rainbow trout (Oncorhynchus mykiss)

The effect of β-hydroxy-β-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves

The effect of β-hydroxy-β-methylbutyrate (HMB) on selected parameters of humoral immunity in calves

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