In vitro exposure to very low-level laser modifies expression level of extracellular matrix protein RNAs and mitochondria dynamics in mouse embryonic fibroblasts

Giuliani, Alessandro; Lorenzini, Luca; Alessandri, Marco; Torricella, Roberta; Baldassarro, Vito Antonio; Giardino, Luciana; Calzà, Laura

doi:10.1186/s12906-015-0593-8

Cited by 13 publications

(12 citation statements)

References 45 publications

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“…Additionally, PBM of complexes II and III still occurs with higher fluence lasers, despite previous concerns that the increased level of stimulation would be too strong 37. Though the focus has primarily been on enhanced effects of mitochondrial function, some have suggested that pulsed t-PBM may also increase the number of isolated mitochondria 38…”

Section: Proposed Mechanisms Of T-pbmmentioning

confidence: 99%

<p>Transcranial Photobiomodulation For The Management Of Depression: Current Perspectives</p>

Askalsky¹,

Iosifescu²

2019

NDT

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Major depressive disorder (MDD) is a prevalent condition associated with high rates of disability, as well as suicidal ideation and behavior. Current treatments for MDD have significant limitations in efficacy and side effect burden. FDA-approved devices for MDD are burdensome (due to repeated in-office procedures) and are most suitable for severely ill subjects. There is a critical need for device-based treatments in MDD that are efficacious, well-tolerated, and easy to use. In this paper, we review a novel neuromodulation strategy, transcranial photobiomodulation (t-PBM) with near-infrared light (NIR). The scope of our review includes the known biological mechanisms of t-PBM, as well as its efficacy in animal models of depression and in patients with MDD. Theoretically, t-PBM penetrates into the cerebral cortex, stimulating the mitochondrial respiratory chain, and also significantly increases cerebral blood flow. Animal and human studies, using a variety of t-PBM settings and experimental models, suggest that t-PBM may have significant efficacy and good tolerability in MDD. In aggregate, these data support the need for large confirmatory studies for t-PBM as a novel, likely safe, and easy-to-administer antidepressant treatment.

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Section: Proposed Mechanisms Of T-pbmmentioning

confidence: 99%

<p>Transcranial Photobiomodulation For The Management Of Depression: Current Perspectives</p>

Askalsky¹,

Iosifescu²

2019

NDT

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“…This subunit acts as a receptor for the laminin family. In a study conducted by Giuliani and colleagues [ 67 ] the daily irradiation of mouse embryonic fibroblasts for 3 days to a wavelength of 670 nm (pulsed wave, 0.21 mW/cm 2 , 4.3 mJ/cm 2 ) resulted in the up-regulation of ITGA5 . This is in contract to our study, but may be due to the different irradiation protocols.…”

Section: Discussionmentioning

confidence: 99%

“…In a similar study, diabetic wounded fibroblast cells irradiated at the same parameters as in this study (660 nm; 5 J/cm 2 ) showed increased secretion of EGF, and subsequent activation of its receptor, EGFR [ 57 ]. Giuliani and colleagues [ 67 ] found no effect on the gene regulation of LAMA1 when they irradiated mouse embryonic fibroblasts (670 nm, 4.3 mJ/cm 2 ). They also found no effect on cadherin 1.…”

Section: Discussionmentioning

confidence: 99%

Cell Adhesion Molecules Are Mediated by Photobiomodulation at 660 nm in Diabetic Wounded Fibroblast Cells

Houreld

Ayuk

Abrahamse

2018

Cells

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Diabetes affects extracellular matrix (ECM) metabolism, contributing to delayed wound healing and lower limb amputation. Application of light (photobiomodulation, PBM) has been shown to improve wound healing. This study aimed to evaluate the influence of PBM on cell adhesion molecules (CAMs) in diabetic wound healing. Isolated human skin fibroblasts were grouped into a diabetic wounded model. A diode laser at 660 nm with a fluence of 5 J/cm2 was used for irradiation and cells were analysed 48 h post-irradiation. Controls consisted of sham-irradiated (0 J/cm2) cells. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of CAM-related genes. Ten genes were up-regulated in diabetic wounded cells, while 25 genes were down-regulated. Genes were related to transmembrane molecules, cell–cell adhesion, and cell–matrix adhesion, and also included genes related to other CAM molecules. PBM at 660 nm modulated gene expression of various CAMs contributing to the increased healing seen in clinical practice. There is a need for new therapies to improve diabetic wound healing. The application of PBM alongside other clinical therapies may be very beneficial in treatment.

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“…This test was performed by comparing Biolite emission with those of two lasers with the same wavelength and spot size but with different power/modulation (one at the same peak power but with no modulation and the other at 50 mW but with no modulation). Surprisingly enough, the Biolite effectiveness was significantly higher than the others (in press) [45]. (2) Effectiveness of Biolite emission in reinforcing the antibiotic treatment of Staphylococcus aureus [46].…”

Section: Biolite Developmentmentioning

confidence: 99%

Biolite: A Patented Ultra-Low-Level Laser-Therapy Device for Treating Musculoskeletal Pain and Associated Impairments

Gallamini¹,

D’Angelo

Belloni³

2015

Journal of Acupuncture and Meridian Studies

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After an excursus on state-of-the-art knowledge for low-level laser therapy (LLLT), Biolite, a patented ultra-low-level laser therapy device used to treat musculoskeletal pain and associated impairments, is presented. The application protocols include short stimulation of sequences of acupuncture points. The observed effects seem, however, to be far from those that might be expected after acupuncture. The primary effect seems more likely to be an extracellular soft-tissue matrix reaction. The development of the technique, the studies performed, and the evidence collected over > 10 years suggest that specifically modulated laser light can interact with human tissues at light fluences well under those previously considered as being capable of having any effect. Musculoskeletal pain very often becomes an autonomous dysfunction that is independent of the original injury and that can be effectively treated using specific peripheral acupuncture-like stimulation. Because such acupuncture is capable of reducing motor control "interferences" from noxious stimuli, it can improve motor control performance, thereby reducing the risk of falls in the elderly individuals. The proposal of acupuncture-derived protocols to be applied by Western physiotherapists using an ultra-low-level laser therapy device is a further "bridge" between two different, and sometimes very different, clinical worlds to better serve our patients.

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In vitro exposure to very low-level laser modifies expression level of extracellular matrix protein RNAs and mitochondria dynamics in mouse embryonic fibroblasts

Cited by 13 publications

References 45 publications

<p>Transcranial Photobiomodulation For The Management Of Depression: Current Perspectives</p>

<p>Transcranial Photobiomodulation For The Management Of Depression: Current Perspectives</p>

Cell Adhesion Molecules Are Mediated by Photobiomodulation at 660 nm in Diabetic Wounded Fibroblast Cells

Biolite: A Patented Ultra-Low-Level Laser-Therapy Device for Treating Musculoskeletal Pain and Associated Impairments

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