In vitro expansion of human beta cells

Efrat, Shimon

doi:10.1007/s00125-008-1024-x

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…Therefore, the formation of functional new β-cells is considered to be an ultimate cue for diabetes therapy. Strategies for increasing the β-cell mass include inhibiting β-cell apoptosis and stimulating β-cell replication of original cells, as well as regeneration from embryonic stem/progenitor cells4243. In both cases, creating an ex vivo microenvironment for β-cells is critical to enhance their survival and maintain their phonotype.…”

Section: Discussionmentioning

confidence: 99%

Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells

Lee

et al. 2013

Sci Rep

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Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types.

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Section: Discussionmentioning

confidence: 99%

Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells

Lee

et al. 2013

Sci Rep

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“…A cultura de ilhotas humanas adultas resultou numa pequena taxa proliferativa e perda da expressão de insulina (Beattie et al, 2000;Beattie et al, 1999;Beattie et al, 2002;Halvorsen et al, 2000). Na ausência de marcadores estáveis para células β em cultura, não foi possível determinar o quanto a perda do fenótipo em células expandidas reflete a desdiferenciação de células β ou a morte celular acompanhada pela expansão de células da ilhota a partir de outros tipos celulares (Efrat, 2008b).…”

Section: Fontes Alternativas De Células ß Para O Transplanteunclassified

Ilhotas pancreáticas humanas viáveis para o transplante através do aumento da massa de células e do imunoisolamento com microcápsulas biocompatíveis

Campos-Lisbôa¹

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Agradeço aos meus pais, Márcio e Marisa, pelo amor incondicional e confiança, os quais me fizeram ter forças sempre para levantar a cabeça, sacudir a poeira e seguir em frente. "O essencial é invisível aos olhos, só se vê bem com o coração" Antoine de Saint-Expuréy. Agradeço ao meu esposo Marcílio, por ter sido companheiro de sonhos e já de algumas realizações. Obrigada por acreditar em mim, por acreditar que valia a pena, e por isso, obrigada pela espera. "(...)que seja infinito enquanto dure" Vinícius de Moraes. Obrigada aos meus irmãos e melhores amigos, Marília Gabriela e Márcio Henrique, pelo apoio incondicional. Vocês são para mim o ontem, o hoje e o sempre. Um porto seguro, amo muito vocês. "Viver e não ter a vergonha de ser feliz" Gonzaguinha.

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