In vitro evidence for the antibacterial role of lysozyme in salmonid eggs

Yousif, Alex N; Albright, L. J.; Evelyn, T. P. T.

doi:10.3354/dao019015

Cited by 92 publications

(59 citation statements)

References 21 publications

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“…Previous studies on several fish species have shown that maternal IgM is able to be transferred from mother to offspring (4 -13). Likewise, maternal transfer of the innate immune factors, including the complement component C3 (14 -19), lectins (20 -22), protease inhibitors (23,24), and lysozymes (25,26), to offspring has also been reported in different teleost species. Moreover, immunization of parents results in a significant increase in IgM levels (7,12) and anti-protease and lysozyme activities (7) in the eggs compared with control.…”

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confidence: 99%

Phosvitin Plays a Critical Role in the Immunity of Zebrafish Embryos via Acting as a Pattern Recognition Receptor and an Antimicrobial Effector

Wang

et al. 2011

Journal of Biological Chemistry

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How fish embryos that develop externally survive microbial attacks is poorly understood. Here, we clearly demonstrated that the embryo extract of zebrafish and its early embryo both displayed antimicrobial activity against microbes, including pathogenic Aeromonas hydrophila, and phosvitin (Pv), a nutritional protein abundant in eggs, was related to this antimicrobial activity. We also showed that recombinant Pv (rPv) acted as a pattern recognition receptor capable of recognizing the microbial signature molecules LPS, lipoteichoic acid, and peptidoglycan, as well as binding the Gram-negative and -positive microbes Escherichia coli, A. hydrophila, and Staphylococcus aureus and functioned as an antimicrobial agent capable of killing the microbes. Furthermore, we revealed that its C-terminal 55 residues (Pt5) with the functional sites Arg 242 and Ala 201 / Ile 203 were indispensable for Pv antimicrobial activity. Importantly, microinjection of rPv or Pt5 into early embryos significantly enhanced their resistance to A. hydrophila challenge, and this enhanced bacterial resistance was markedly reduced by coinjection of anti-Pv antibody plus rPv (or Pt5) but not by injection of anti-actin antibody plus rPv. Moreover, the generated mutants with in vitro antimicrobial activity, when injected into the embryos, could also promote their resistance to A. hydrophila, but those without in vitro antimicrobial activity could not. It is thus proposed that Pv participates in the protection of early embryos against pathogenic attacks via binding and disrupting potential pathogens. This work also opens a new way for the study of the immunological roles of yolk proteins in oviparous animals that rely on yolk proteins for embryonic development.

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confidence: 99%

Phosvitin Plays a Critical Role in the Immunity of Zebrafish Embryos via Acting as a Pattern Recognition Receptor and an Antimicrobial Effector

Wang

et al. 2011

Journal of Biological Chemistry

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“…Although, the variations of the immune parameters such as the lysozyme, C3 and IgM in the larval stages of some teleost species have been studied, there is no such information for the sturgeons. The existence of maternal lysozyme has recently been confirmed in the oocytes, fertilized eggs and larval stages of Coho salmon (Oncorhynchus kisutch) (Yousif, Albright, & Evelyn, 1994), red sea bream (Pagrus major) (Kanlis, Suzuki, Tauchi, Numata, & Shirojo, 1995) and tilapia (Oreochromis niloticus) (Sin, Ling, & Lam, 1994). It had been shown that the lysozyme in the unfertilized eggs had higher level than the fertilized eggs (1 HPF) and 6 DPH larvae (Cecchini, Terova, Caricato, & Saroglia, 2000).…”

Section: Discussionmentioning

confidence: 97%

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Zamaninia¹,

Azizi²,

Heidari³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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In this study, some important immune parameters in the unfertilized and fertilized eggs, larvae of 10 days post hatching (DPH), juveniles of 1 to 12 month post fertilization (MPF) and the female broodstock were evaluated. In the Persian sturgeon, there was a significant decrement in the lysozyme activity from the unfertilized eggs to the 10 DPH larvae and after that, the remarkable variations were not observed. The C3 concentration did not show any significant variations from the unfertilized eggs to the 1 MPF and after that, it significantly decreased. Apart from the fertilized eggs, the variations of the IgM level were similar to the C3 level in the early life stages of the Persian sturgeon. In the Sterlet, the lysozyme level was decreased significantly from the unfertilized eggs to 10 DPH larvae, then it was increased in 1 MPF and finally, a significant difference was not observed from 2 to 12 MPF. The variations of both the IgM and the C3 levels were similar from the fertilized eggs to 12 MPF. In conclusion, the considerable fluctuations of the immune parameters were observed in early life stages especially under 1 MPF which indicates immunologically sensitivity of the fish in these stages.

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“…Also, the presence of lysozyme activity in plasma, skin mucus, organs and fish egg has been well documented in different fish species (Engstad et al, 1992;Fletcher and White, 1973;Yousif et al, 1994;Paulsen et al, 2003) The increase of lysozyme contents by an elevation in lysozyme mRNA suggests that the stimulated lysozyme secretion is started by enhanced lysozyme gene transcription (Paulsen et al, 2003). Immunstimulant agents such as β-glucan and LPS stimulated increase in lysozyme activity and gene transcript in Atlantic salmon macrophages (Paulsen et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Heidarieh¹,

Soltani²,

Tamimi³

et al. 2011

Turk. J. Fish. Aquat. Sci.

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The imunnocompetent cell population size including lymphocyte, neutrophil and eosinophil populations, lysozyme activity in plasma and the growth performance were assessed in great sturgeon Huso huso after oral administration of Ergosan at 0.5% of the feed and Vitacel at 1.3% of the feed for 90 days. Sampling was performed on 15 and 90 days during feeding trial. Generally, a significant increase was seen in the lymphocyte count of fish received Ergosan compared to both Vitacel and control groups 90 days post-treatment (P<0.05). Interstingly, at the Neutrophil profile of fish fed Vitacel and VitacelErgosan combination was observed significant increase on 90 days after trial (P<0.05). The number of eosinophils tended to be higher in Vitacel group (P<0.05) on day 90 and was significantly higher (P<0.05) than in Ergosan and control. Also, lysozyme activity in plasma of treatment fish by Ergosan and Vitacel was significantly higher than control group on 15 and 90 days post-treatment (P<0.05). In addition, the weight of fish fed diet containing Ergosan and/or Vitacel had a significant increase compared to control group on days 15 and 90 of trial (P<0.05).Keywords: Ergosan, Vitacel, Huso huso, Lysozyme, Growth performance. Gelişkin Mersin Balıklarının (Huso

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In vitro evidence for the antibacterial role of lysozyme in salmonid eggs

Cited by 92 publications

References 21 publications

Phosvitin Plays a Critical Role in the Immunity of Zebrafish Embryos via Acting as a Pattern Recognition Receptor and an Antimicrobial Effector

Phosvitin Plays a Critical Role in the Immunity of Zebrafish Embryos via Acting as a Pattern Recognition Receptor and an Antimicrobial Effector

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