In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism Ovatisporangium (Phytopythium) Isolate Recovered from Tomato Fields in Iran

Abstract

Crop diseases and agricultural pests and pathogens are causing huge economic losses. The actual means for dealing with them involve the use of damaging chemical pesticides that harm the environment, threaten biodiversity, and undermine human health. This research was aimed at developing an environmentally friendly means to cope with emerging oomycete disease from tomato fields in the province of East-Azerbaijan. The oomycete disease causal agent was isolated and identified as Ovatisporangium sp. using a combination of morphological features and molecular methods. Six wood vinegars (pyroligneous acid) belonging to pine, pomegranate, pistachio, almond, walnut, and cypress were produced during this study and examined against Ovatisporangium sp. Their inhibition of volatile metabolites (VOCs) using different dilutions (1, 1/2, 1/4, 1/8, and 1/10) was assessed against the mycelial growth of Ovatisporangium sp. In vitro analysis demonstrated that pistachio, cypress, and almond dilution 1 (D 1) wood vinegar VOCs had the ability to stop the mycelial growth of Ovatisporangium sp. All other treatments including pine, walnut, and pomegranate with relevant dilutions significantly reduced the mycelial growth of Ovatisporangium sp. compared with the control (p ≤ 0.05). Wood vinegar is therefore a potent means to cope with pathogenic infections and allows plant protection against oomycete diseases.

Optimizing Cucumis sativus seedling vigor: the role of pistachio wood vinegar and date palm compost in nutrient mobilization

“…The tree pistachio was selected in this study. Pyroligneous acid was extracted following the procedure described by Bouket et al [ 37 ]…”

Perspective Chapter: Bioconversion of Agricultural and Food Wastes to Vinegar

“…Pyrolyzed apple tree branch wood vinegar -Broadleaf weed was controlled [154] -Controlled annual bluegrass [74] Wood vinegar Ulmus spp. tree pruning waste -Broadleaf weed was controlled [75] Wood vinegar (pistachio, cypress, and almond) -reduced the mycelial growth of the funguslike organism Ovatisporangium sp [155] Wood vinegar (Toona sinensis)…”

Physiological and Biochemical Mechanisms of Wood Vinegar-Induced Stress Response against Tomato Fusarium Wilt Disease

“…Wood vinegar has been used extensively in agriculture, mainly for soil improvement, promotion of plant growth, such as vegetables and fruit trees, improvement of fruit taste and quality, and replacement of synthetic pesticides. As a new type of green natural product, wood vinegar not only degrades easily, but also contains acids and active phenolic substances with insecticidal and antibacterial effects [11]. Tomato (Solanum lycopersicum) is one of the most important and widely cultivated vegetable crops worldwide but it is threatened by wilt disease during growth [12].…”