In vitro Evaluation of the Antibacterial Potential of Niosome-Encapsulated Olive Leaf Extract

Abstract

Olive leaf extract (OLE) loaded niosomes as an antibacterial agent delivery system against Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Listeria innocua (ATCC 33090) and Methicillin-resistant Staphylococcus aureus (MRSA) were investigated by comparing with the antibacterial activity of free OLE. Niosomes were prepared using a lipid film hydration method followed by ultrasound-assisted size reduction technique and characterized in terms of morphology, entrapment efficiency (41.92±0.81%), average particle size (2.66±1.25 μm) and zeta-potential (-34.16±6.48 mV). The minimum inhibitory concentrations (MIC) of free OLE and OLE loaded niosomes against strains were determined. The most susceptible strain against free OLE was the MRSA with MIC value of 2.34 mg/mL OLE. However, the MIC value of OLE loaded niosomes against MRSA was 100 mg/mL. Unfortunately, OLE loaded niosomes (150 mg) with max capacity did not show any antibacterial effect against other three strains due to entrapment efficiency. In conclusion, OLE loaded niosomes has promising value as a novel antimicrobial delivery system to control MRSA.