In vitro evaluation of the action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs

Braga, Fábio Ribeiro; Araújo, Jackson Víctor de; Campos, Artur Kanadani; Carvalho, Rogério Oliva; Silva, A. R.; Tavela, A.O.

doi:10.1007/s11274-007-9643-9

Cited by 45 publications

(25 citation statements)

References 16 publications

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“…chlamydosporia has proven action against eggs of parasitic nematodes of animals and plants (Lopes et al 2007;Araújo et al 2008). Recent studies, under laboratory conditions, have shown that it can also destroy eggs of trematodes, cestodes and nematodes parasitizing domestic animals (Braga et al 2007(Braga et al , 2008aAraújo et al 2008).…”

Section: Discussionmentioning

confidence: 99%

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Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs

Ferreira

Araújo

Braga

et al. 2010

Trop Anim Health Prod

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The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water-agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p>0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.

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Section: Discussionmentioning

confidence: 99%

“…Nematophagous fungi are classified as endoparasites, predators and parasites of nematode eggs (Araújo et al 2004(Araújo et al , 2008. Pochonia chlamydosporia colonizes and destroys helminth eggs using appressoria developed from undifferentiated hyphae (Braga et al 2007(Braga et al , 2008aAraújo et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs

Ferreira

Araújo

Braga

et al. 2010

Trop Anim Health Prod

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“…P. chlamydosporia has previously been shown to be ovicidal against eggs of trematodes, cestodes and nematodes in vitro under laboratories conditions (Araujo et al, 2009;Braga et al, 2007Braga et al, , 2008, however, this is the first report of its ovicidal activity against eggs of D. renale. The production of chlamydospores, resistant structure, is one of the main characteristics of a fungus, which possibly could be used in environmental control of Biocontrol Science and Technology 403 parasitic gastrointestinal helminth eggs (Braga et al, 2007).…”

Section: Discussionmentioning

confidence: 74%

Action of the nematophagous fungusPochonia chlamydosporiaonDioctophyma renaleeggs

Soares

Queiróz

Braga

et al. 2014

Biocontrol Science and Technology

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The ovicidal action of the nematophagous fungus Pochonia chlamydosporia (VC4) was evaluated on Dioctophyma renale eggs under laboratory conditions (Assay A). Next, the enzymatic action of proteases and chitinases produced by P. chlamydosporia (VC4) was evaluated on D. renale eggs, under laboratory conditions (Assay B). At the end of the experiment, there was difference (p < 0.01) in the destruction of eggs in the four concentrations tested in relation to control group at each interval studied. On the other hand, no difference was observed (p > 0.01) among the concentrations in the destruction of eggs. However, there was a trend of increasing mortality with increased concentration. Then (Assay B), it was observed that in the 24-hour interval, the proteases and chitinases of P. chlamydosporia (VC4), either individually or together, caused a significant percentage reduction (p < 0.01) on the number of viable eggs of D. renale, compared to control, with the following reduction values: 27.8% (proteases), 29.4% (chitinases) and 43.4% (proteases + chitinases). Thus, the constant search for alternatives that may help combat the various infectious forms (or eggs and larvae) of potentially zoonotic nematodes is important, as in the use of fungi destroyers of eggs. Therefore, it is suggested that the application of P. chlamydosporia would be an approach in the biological control of nematodes.

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“…This might be due to that the isolates had lytic effect on the eggs wall without morphological damage. In this sense, Braga et al [20] found that the fungi Duddingtonia flagrans and Monacrosporium sinense had shown lytic effect on F. hepatica egg wall without its morphological damage reached 66.5 and 73.4%, respectively. Also, the same author reported the same effect on eggs of Ascris lumbricoides.…”

Section: Discussionmentioning

confidence: 99%

“…At present, effective and commercial vaccines are not yet available; therefore anthelmintic drugs are the main method employed for controlling the fluke infection [19]. However, due to emergence of resistance and the cost of animal treatment, especially for small ruminants, the use of new control alternative measures is important, especially of the present eggs in the pasture, such as biological control [20]. The use of bacteria appears therefore as a viable and promising control alternative for constantly infected animals.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Action of Streptomyces griseolus Proteases as Bio-Control on Fasciola gigantic Eggs

Gammal¹,

Shalaby²

2014

J Bacteriol Parasitol

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This work evaluated the in vitro bio-control action of actinomycetes isolated from Egyptian soil against Fasciola gigantica eggs. In this respect, the identified Streptomyces griseolus was the best parasite-control agent which gave the highest mortality percent applying three methods; the first method was the application of the grown bacterial disks with the eggs at zero time on the medium (2% w/v water-agar) and incubated for 21 days (75.5% mortality), the second method was the incubation of the bacterial disks using the same medium for 5 days before eggs inoculation, after 21 days of incubation the mortality percent was 80.8%. The application of the third method showed a good effect by using all bacterial culture filtrates. S. griseolus filtrate which had the highest lytic enzyme and proteolytic activities reached 2.0 and 660.7 U/ml, respectively, gave the highest mortality (95.5%) of F. gigantica eggs compared to the other bacterial filtrates. The crude culture filtrate and the diluted culture filtrate (1.33 fold) of S. griseolus gave the highest mortality percent against the parasite´s eggs (95.8 and 94.5%, respectively) compared to the higher diluted filtrates. The culture filtrate of S. griseolus grown on two different media and its partially purified enzyme (precipitated by 60% ammonium sulfate) were tested for their proteolytic activity and inhibitory effect against F. gigantica eggs. Medium no. 2 showed the highest proteolytic activity (1030.3 and 1138.1 U/ml) for the crude and the partially purified enzyme, respectively. While the highest inhibitory effect reached 97.6 and 89.9%, respectively. Finally, the application of crude enzyme filtrate was better than the partially purified one. Streptomyces griseolus was proved as a potential biological control agent for this helminth.

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In vitro evaluation of the action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs

Cited by 45 publications

References 16 publications

Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs

Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs

Action of the nematophagous fungusPochonia chlamydosporiaonDioctophyma renaleeggs

In Vitro Action of Streptomyces griseolus Proteases as Bio-Control on Fasciola gigantic Eggs

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