In Vitro Evaluation of the Cytotoxicity of Two Root Canal Sealers on Macrophage Activity

Mendes, Sônia Teresa de Oliveira; Sobrinho, Antônio Paulino Ribeiro; Carvalho, André Teixeira de; Côrtes, Maria Ilma de Souza; Vieira, Leda Quércia

doi:10.1097/00004770-200302000-00002

Cited by 42 publications

(52 citation statements)

References 15 publications

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“…According to Oliveira Mendes et al (21), although zinc oxide-eugenol sealers did not interfere with the macrophages' viability, their adherence and phagocytosis are affected in contact with this sealer, which could explain giant cells mobilization to eliminate the material. Moreover, after 60 days, Endofill showed significant higher scores for macrophages compared to the control group and presented a tendency for higher scores of neutrophils and abscess.…”

Section: Discussionmentioning

confidence: 97%

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

et al. 2016

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Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

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Section: Discussionmentioning

confidence: 97%

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

et al. 2016

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“…The preservation of cytoplasmic extensions is important, because these extensions form a three-dimensional network within hard tissue and materials [16,31,[36][37][38]. The factors that affect cellular response to calcium phosphates include surface topography (roughness), composition and particle size.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

Gala-García

Carneiro

Silva

et al. 2012

J Mater Sci: Mater Med

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This study assess the effects of bioceramic and poly(lactic-co-glycolic acid) composite (BCP/PLGA) on the viability of cultured macrophages and human dental pulp fibroblasts, and we sought to elucidate the temporal profile of the reaction of pulp capping with a composite of bioceramic of calcium phosphate and biodegradable polymer in the progression of delayed dentine bridge after (30 and 60 days) in vivo. Histological evaluation of inflammatory infiltrate and dentin bridge formation were performed after 30 and 60 days. There was similar progressive fibroblast growth in all groups and the macrophages showed viability. The in vivo study showed that of the three experimental groups: BCP/PLGA composite, BCP and calcium hydroxide (Ca(OH)(2)) dentin bridging was the most prevalent (90 %) in the BCP/PLGA composite after 30 days, mild to moderate inflammatory response was present throughout the pulp after 30 days. After 60 days was observed dentine bridging in 60 % and necrosis in 40 %, in both groups. The results indicate that understanding BCP/PLGA composite is biocompatible and by the best tissue response as compared to calcium hydroxide in direct pulp capping may be important in the mechanism of delayed dentine bridge after 30 and 60 days.

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“…It has been demonstrated that lower concentrations of eugenol can easily reach an effective cytotoxic level on USO2 cells during longterm exposure. Eugenol is capable of inhibiting the function of macrophages and thus affects the inflammation of periapical tissues (11).…”

Section: Scientific Journal Of the Faculty Of Medicine In Niš 2015;32mentioning

confidence: 99%

Bone Tissue Response to Endomethasone Implanted into the Rat Mandible / Reakcija koštanog tkiva na endometazon implantiran u mandibulu pacova

Nikolić¹,

Gasic²,

Mitić³

et al. 2015

Acta Facultatis Medicae Naissensis

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The aim of the paper was to investigate the tissue response to the implantation of "Endomethasone" (Septodont) in artificially prepared defect in the rat mandible.Sixteen Wistar male rats were used for the experiment. After anesthesia, a defect between the midline and the mental foramen of the left mandible was made (diameter 1.4 mm, 1.6 mm depth) using sterile steel burs. In the defect of the experimental group (12 animals) "Endomethasone" was implanted while the defect in the control group (4 animals) was left to heal spontaneously. One half of animals of both groups was sacrificed after fifteen days, and the second half after sixty days. The samples consisted of the defect and the surrounding bone. After routine decalcination and processing, the samples were embedded in paraffin, and microscopic preparations were made, on the basis of which a microscopic analysis was performed.Fifteen-day-old specimens showed the early signs of bone resorption, as well as granulated connective tissue, hyperemic blood vessels, fibrin exudate to a lesser extent, with the difference that inflammatory infiltrates were less present in the experimental group. Sixty-day-old specimens demonstrated the degree of bone healing; tissue filling with newly formed bone was significantly more advanced in the control group. Experimental group showed a sign of delay with significant presence of callus tissue that more in the form of focus showed a deposition of the young bone tissue.Endomethasone masks the inflammatory reaction which follows operational procedures. In the long run, according to the results of the present research, Endomethasone can interfere with bone healing by causing delayed inflammatory reaction.

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In Vitro Evaluation of the Cytotoxicity of Two Root Canal Sealers on Macrophage Activity

Cited by 42 publications

References 15 publications

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

Bone Tissue Response to Endomethasone Implanted into the Rat Mandible / Reakcija koštanog tkiva na endometazon implantiran u mandibulu pacova

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