In vitro evaluation of the role of platelet-activating factor and interleukin-8 in Mannheimia haemolytica-induced bovine pulmonary endothelial cell injury

McClenahan, David; Evanson, Oral A.; Weiss, Douglas J.

doi:10.2460/ajvr.2002.63.394

Cited by 8 publications

(5 citation statements)

References 56 publications

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“…Higher concentrations of IL-8 were found in lesional lung tissue of BHS than in nonlesional lung tissue of BHS and DS by 18 hpi. The presence of higher IL-8 concentrations in lesional lung tissue is consistent with the results from similar Given the ability of IL-8 to induce PMN activation, degranulation, and endothelial cell damage (19,21,23), a large population of PMNs producing high levels of IL-8 at the site of inflammation is likely responsible for the severe lung damage observed in M. haemolytica-infected BHS in comparison to that observed in DS.…”

Section: Discussionsupporting

confidence: 78%

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Differential Expression of Interleukin-8 by Polymorphonuclear Leukocytes of Two Closely Related Species, Ovis canadensis and Ovis aries , in Response to Mannheimia haemolytica Infection

2010

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The pneumonic lesions and mortality caused by Mannheimia haemolytica in bighorn sheep (BHS; Ovis canadensis) are more severe than those in the related species, domestic sheep (DS; Ovis aries), under both natural and experimental conditions. Leukotoxin ( Pneumonia caused by Mannheimia haemolytica is an important factor in the drastic decline of bighorn sheep (BHS; Ovis canadensis) populations in North America (3, 31). The pneumonic lesions and mortality caused by this organism in BHS are much more severe than those in the related species, domestic sheep (DS; Ovis aries), under both natural and experimental conditions (11,12,13,24). The cellular and molecular basis underlying the differential pathogenicity exhibited by M. haemolytica in BHS and DS has not been elucidated yet.

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Section: Discussionsupporting

confidence: 78%

“…Neutralization of IL-8 also reduced the degrees of both pulmonary arterial endothelial cell (PAEC) damage and PMN degranulation in a coculture system containing bovine PAEC and PMNs (21). Taken together, these results clearly implicate IL-8 as an important mediator of both PMN influx and host cell damage during M. haemolytica infection.…”

Section: Discussionsupporting

confidence: 55%

Differential Expression of Interleukin-8 by Polymorphonuclear Leukocytes of Two Closely Related Species, Ovis canadensis and Ovis aries , in Response to Mannheimia haemolytica Infection

2010

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“…101 LPS can cause pulmonary damage directly through toxic effects on pulmonary endothelium and indirectly through neutrophil recruitment. 55,85,97 The toxicity of LPS can be enhanced by complexing it with the phospholipids in the pulmonary surfactant, which allow LPS to persist in the lung and initiate inflammation. 12,90 Other systemic effects induced by LPS include fever and production of acute-phase proteins by the liver.…”

mentioning

confidence: 99%

“…Current evidence indicates that LPS contributes to the pulmonary lesions through a variety of complex mechanisms, including the stimulation of leukocytes to produce proinflammatory cytokines, the activation of complement and coagulation cascade, and direct cell cytolysis. 69,83,85 The LPS molecule consists of polysaccharide side chain (O antigen), lipid A, and inner and outer cores of oligosaccharides, with the size of the immunogenic side chain determining whether the LPS type has a rough or smooth material. 8,67 The lipid A moiety of LPS is responsible for eliciting endotoxic effects, such as pyrexia and hypotensive shock.…”

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confidence: 99%

Mannheimia haemolytica

2010

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Mannheimia haemolytica serotype S1 is considered the predominant cause of bovine pneumonic pasteurellosis, or shipping fever. Various virulence factors allow M haemolytica to colonize the lungs and establish infection. These virulence factors include leukotoxin (LKT), lipopolysaccharide, adhesins, capsule, outer membrane proteins, and various proteases. The effects of LKT are species specific for ruminants, which stem from its unique interaction with the bovine b2 integrin receptor present on leukocytes. At low concentration, LKT can activate bovine leukocytes to undergo respiratory burst and degranulation and stimulate cytokine release from macrophages and histamine release from mast cells. At higher concentration, LKT induces formation of transmembrane pores and subsequent oncotic cell necrosis. The interaction of LKT with leukocytes is followed by activation of these leukocytes to undergo oxidative burst and release proinflammatory cytokines such as interleukins 1, 6, and 8 and tumor necrosis factor a. Tumor necrosis factor a and other proinflammatory cytokines contribute to the accumulation of leukocytes in the lung. Formation of transmembrane pores and subsequent cytolysis of activated leukocytes possibly cause leakage of products of respiratory burst and other inflammatory mediators into the surrounding pulmonary parenchyma and so give rise to fibrinous and necrotizing lobar pneumonia. The effects of LKT are enhanced by lipopolysaccharide, which is associated with the release of proinflammatory cytokines from the leukocytes, activation of complement and coagulation cascade, and cell cytolysis. Similarly, adhesins, capsule, outer membrane proteins, and proteases assist in pulmonary colonization, evasion of immune response, and establishment of the infection. This review focuses on the roles of these virulence factors in the pathogenesis of shipping fever.

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“…In neutrophils, PAF promotes [Ca 2+ ] i release, reactive oxygen species, granule enzymes [3], as well as extracellular acidification and intracellular alkalinization [4]. Since PAF has been involved in many inflammatory processes [5], [6], several PAF receptor antagonists have been identified, either synthetic (i. e., WEB2086, CV-3988) or natural (ginkgolides, kadsurenone) [7].…”

Section: Introductionmentioning

confidence: 99%

14-Deoxyandrographolide as a Platelet Activating Factor Antagonist in Bovine Neutrophils

Burgos

Hidalgo²,

Monsalve³

et al. 2005

Planta med

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14-Deoxyandrographolide (14-DAP) is a labdane diterpene isolated from Andrographis paniculata with previously reported calcium channel blocking activity. Its potential platelet activating factor (PAF) antagonistic activity in bovine neutrophils was assessed. 14-DAP, in concentrations between 10-100 microM, reduced the extracellular acidification rate and the intracellular alkalinization in a dose-dependent manner. In addition, 14-DAP reduced PAF-induced calcium flux in the presence of extracellular calcium, and tyrosine phosphorylation of a 44 kDa protein corresponding to the MAPK(ERK1). However, 14-DAP reduced the 3H-PAF binding with a Ki of 7.8 x 10 (- 9)M, and a Hill slope of 0.63, suggesting that there is more than one binding site for 14-DAP. We concluded that 14-DAP is an effective antagonist of PAF-mediated processes in bovine neutrophils, probably by virtue of its calcium channel blocking property.

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In vitro evaluation of the role of platelet-activating factor and interleukin-8 in Mannheimia haemolytica-induced bovine pulmonary endothelial cell injury

Cited by 8 publications

References 56 publications

Differential Expression of Interleukin-8 by Polymorphonuclear Leukocytes of Two Closely Related Species, Ovis canadensis and Ovis aries , in Response to Mannheimia haemolytica Infection

Differential Expression of Interleukin-8 by Polymorphonuclear Leukocytes of Two Closely Related Species, Ovis canadensis and Ovis aries , in Response to Mannheimia haemolytica Infection

Mannheimia haemolytica

14-Deoxyandrographolide as a Platelet Activating Factor Antagonist in Bovine Neutrophils

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