In Vitro Evaluation of the Effects of Intraocular Lens Material on Lens Epithelial Cell Proliferation, Migration, and Transformation

Wang, Guiqin; Dang, Yalong; Huang, Qi; Woo, Victor C.P.; So, Kwok-Fai; Lai, Jimmy Shiu Ming; Cheng, Grand H.‐L.; Chiu, Kin

doi:10.3109/02713683.2016.1156133

Cited by 13 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro TM cell motility was using a scratch assay with a minor modification 27 . In brief, 2×10 5 of primary TM cells were seeded into each well of a six-well plate and cultured in an environmental chamber that was equipped with a digital camera and image acquisition software.…”

Section: Tm Motility Assaymentioning

confidence: 99%

Ocular Hypotension, Actin Stress Fiber Disruption and Phagocytosis Increase by RKI-1447, a Rho-Kinase Inhibitor

Dang¹,

Wang²,

Shah³

et al. 2018

Preprint

View full text Add to dashboard Cite

Objective: The Rho GTPase/Rho kinase pathway is an important target in glaucoma treatment. This study investigated the hypotensive effect of RKI-1447, a Rho kinase inhibitor developed for cancer treatment, in a porcine ex vivo pigmentary glaucoma model. Materials and Methods:Twenty-eight fresh porcine anterior chambers were perfused with pigment medium (1.67×10 7 pigment particles/ml) for 48 hours before being subjected to the RKI-1447 (n=16) or the vehicle control (n=12). Another twelve eyes with normal medium perfusion served as the control. The intraocular pressure (IOP) was recorded at two-minute intervals and the outflow facility was calculated. To investigate the intracellular mechanism of the IOP reduction, primary trabecular meshwork cells were exposed to RKI-1447 or the vehicle control and then analyzed for changes in cytoskeleton, motility, and phagocytosis.

show abstract

Section: Tm Motility Assaymentioning

confidence: 99%

Ocular Hypotension, Actin Stress Fiber Disruption and Phagocytosis Increase by RKI-1447, a Rho-Kinase Inhibitor

Dang¹,

Wang²,

Shah³

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…High glucose-induced oxidative stress is an important cause of diabetic cataract [2]. The human lens epithelial cell line (HLEB3) was widely used to do research [13][14][15]. Here, using the lens epithelial cell line, we found that high glucose can directly induce the oxidative stress and cell apoptosis.…”

Section: Discussionmentioning

confidence: 90%

Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway

Shao

Xie

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

To determine the effect of decorin on oxidative stress and apoptosis of human lens epithelial (HLE) cells under high glucose condition. Methods HLE cell line (HLEB3) was incubated in normal glucose (5.5 mM) or high glucose (60 mM) medium. Decorin (50 nM) was applied 2 hours before high glucose medium was added. Apoptosis detection was executed by flow cytometry and western blotting (analysis of bcl-2 and bax). Oxidative stress level was measured by the generation of reactive oxygen species (ROS), glutathione peroxidase (GSH) and superoxide dismutase (SOD). P38 mitogen-activated protein kinase (MAPK) phosphorylation, the expression of p22 phox of HLE cells and human lens anterior capsules were detected by western blotting. Small interfering RNA transfection to p22 phox and p38 MAPK was also carried out on HLEB3. Results High glucose caused HLE cells oxidative stress and apoptosis exhibiting the increase of apoptotic cells and ROS production and decrease of bcl-2/bax ratio, GSH/GSSG ration and SOD activity. P22 phox and phospho-p38 MAPK were upregulated in high glucose treated HLEB3 cells. Knocking down p22 phox or p38 by siRNAs can reduce high glucose induced cell apoptosis and oxidative stress level. Silencing p22 phox by siRNA can downregulate the phosphorylation of p38 MAPK. Decorin can inhibit the apoptosis, oxidative stress level and the induction of p22 phox and phospho-p38 of HLEB3 induced by high glucose. Furthermore, the expression of p22 phox and p38 were found significantly increased in lens anterior capsules of diabetic cataract patients compared to that of normal age-related cataract patients.

show abstract

“…The IOL material influences the PCO changes after cataract surgery and can affect the behaviour of LECs. 8,14 The exact mechanism is unknown but could be explained by either mechanical or material related effects.…”

Section: Discussionmentioning

confidence: 99%

Posterior Capsular Changes and Its Association With Posterior Chamber Intraocular Lens Surface Changes After Extracapsular Cataract Extraction

A¹,

Achar²

2017

jemds

View full text Add to dashboard Cite

BACKGROUNDPosterior Capsular Opacification is the most frequent complication of cataract surgery. There are few studies that determine the changes in the lens surfaces with the posterior capsular changes. The objectives of the present study were to report intraocular lens surface changes and posterior capsular changes after extracapsular cataract extraction with posterior chamber intra-ocular lens (ECCE-PCIOL) implantation surgeries, and to determine the factors influencing the anterior surface changes.

show abstract

In Vitro Evaluation of the Effects of Intraocular Lens Material on Lens Epithelial Cell Proliferation, Migration, and Transformation

Abstract: Taken together, these data suggest that IOL composition greatly affects lens cell behavior during PCO development, and it appears that hydrophobic IOLs may be the best option to limit the effects of PCO after cataract surgery while also correcting the patient's vision.

Cited by 13 publications

References 27 publications

Ocular Hypotension, Actin Stress Fiber Disruption and Phagocytosis Increase by RKI-1447, a Rho-Kinase Inhibitor

Ocular Hypotension, Actin Stress Fiber Disruption and Phagocytosis Increase by RKI-1447, a Rho-Kinase Inhibitor

Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway

Posterior Capsular Changes and Its Association With Posterior Chamber Intraocular Lens Surface Changes After Extracapsular Cataract Extraction

Contact Info

Product

Resources

About