In vitro evaluation of prebiotic activity, pathogen inhibition and enzymatic metabolism of intestinal bacteria in the presence of fructans extracted from agave: A comparison based on polymerization degree

García‐Gamboa, Ricardo; Ortíz-Basurto, Rosa Isela; Calderón‐Santoyo, Montserrat; Madrigal, Jorge; Álvarez, Blanca Elizabeth Ruiz; Avila, Marisela González

doi:10.1016/j.lwt.2018.02.051

Cited by 35 publications

(21 citation statements)

References 30 publications

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“…was the most suitable carbon source showing higher bacterial growth compared with CPPF (DPn=2-17) and Orafti ® HP (DPav≥23), while the bacteria in the medium containing Orafti ® HP did not increase comparing to the basal medium (p>0.05) (Table 3). It was shown that the bacterial growth increased with decreasing of the DP, similar with previous reports (Caleffi et al, 2015;Garcí a Gamboa et al, 2018;Lopes et al, 2017;Li et al, 2015;Moreno-Vilet, 2014). In addition, the medium containing CPPF presented lower pH after 24 h incubation in anaerobic chamber compared to the basal medium, except Hjg8 (Table 4), which was consistent with the Δ600 in Table 4.…”

Section: Prebiotic Effectsupporting

confidence: 91%

Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Zhang

et al. 2018

Carbohydrate Polymers

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The inulin-type fructan was obtained by DEAE anion exchange chromatography from C. pilosula Nannf. var. modesta (Nannf.) L. T. Shen, after optimized extract condition, which was established by response surface methodology, designed using Box-Behnken factorial design and the optimum condition were: extracting 2.5 h with ratio of solvent to material 40 mL/g at 100 °C, twice. The maximum extraction yield was 20.6 ± 0.2%. It was confirmed as β-(2-1) linkage fructan, with terminal glucose, and with a degree of polymerization of 2-17 (DP = 6), shown by the results of methanolysis, methylation, nuclear magnetic resonance and molecular weight determination. The prebiotic activity was proven on account of stimulation effect on Lactobacillus and pH reduction of medium in vitro. The results indicated that the inulin from C. pilosula could be used as a potential natural source of prebiotics.

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Section: Prebiotic Effectsupporting

confidence: 91%

Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Zhang

et al. 2018

Carbohydrate Polymers

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“…However, it has been reported that fructans with different DP differ in their prebiotic effectiveness and techno-functional properties, as well as in the removal of low-molecular weight sugars such as glucose, fructose and sucrose, increasing the purity and functionality of fructans. For example, the fraction of agave fructans with DP ˃10 improves the biological effect of triglyceride reduction in relation to native fructans, while the enriched fraction of fructooligosaccharides (FOS) enhances the decrease in body weight, body fat, hyperglycemia and hepatic steatosis [ 11 , 12 , 13 ]. For these reasons, the membrane process has been proposed to obtain agave fructans with a higher purity [ 14 , 15 , 16 ] and to obtain different DP fractions [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Performance Evaluation of Tight Ultrafiltration Membrane Systems at Pilot Scale for Agave Fructans Fractionation and Purification

et al. 2020

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Ceramic and polymeric membrane systems were compared at the pilot scale for separating agave fructans into different molecular weight fractions that help to diversify them into more specific industrial applications. The effect of the transmembrane pressure of ultrafiltration performance was evaluated through hydraulic permeability, permeate flux and rejection coefficients, using the same operating conditions such as temperature, feed concentration and the molecular weight cut-off (MWCO) of membranes. The fouling phenomenon and the global yield of the process were evaluated in concentration mode. A size distribution analysis of agave fructans is presented and grouped by molecular weight in different fractions. Great differences were found between both systems, since rejection coefficients of 68.6% and 100% for fructans with degrees of polymerization (DP) > 10, 36.3% and 99.3% for fructooligosaccharides (FOS) and 21.4% and 34.2% for mono-disaccharides were obtained for ceramic and polymeric membrane systems, respectively. Thus, ceramic membranes are better for use in the fractionation process since they reached a purity of 42.2% of FOS with a yield of 40.1% in the permeate and 78.23% for fructans with DP > 10 and a yield of 70% in the retentate. Polymeric membranes make for an efficient fructan purification process, eliminating only mono-disaccharides, and reaching a 97.7% purity (considering both fructan fractions) with a yield of 64.3% in the retentate.

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“…Five types of fructans have been identified in nature so far, which are classified into inulins, neo-inulins, levans, neo-levans and mixed fructans, depending on the type of bond [3,4]. Agave fructans are characterized by the presence of fructose units with a terminal glucose connected by bonds β (2-1) and β (2)(3)(4)(5)(6) and can present different degrees of polymerization, which are determined by the species [5][6][7] as well as the environmental conditions in which the agave is produced, stored and processed [8,9]. Toriz et al (2007) [10] propose a chemical structure for the fructans of Agave tequilana Weber var.…”

Section: Introductionmentioning

confidence: 99%

Micro- and Nanostructures of Agave Fructans to Stabilize Compounds of High Biological Value via Electrohydrodynamic Processing

et al. 2019

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This study focuses on the use of high degree of polymerization agave fructans (HDPAF) as a polymer matrix to encapsulate compounds of high biological value within micro- and nanostructures by electrohydrodynamic processing. In this work, β-carotene was selected as a model compound, due to its high sensitivity to temperature, light and oxygen. Ultrafine fibers from HDPAF were obtained via this technology. These fibers showed an increase in fiber diameter when containing β-carotene, an encapsulation efficiency (EE) of 95% and a loading efficiency (LE) of 85%. The thermogravimetric analysis (TGA) showed a 90 °C shift in the β-carotene decomposition temperature with respect to its independent analysis, evidencing the HDPAF thermoprotective effect. Concerning the HDPAF photoprotector effect, only 21% of encapsulated β-carotene was lost after 48 h, while non-encapsulated β-carotene oxidized completely after 24 h. Consequently, fructans could be a feasible alternative to replace synthetic polymers in the encapsulation of compounds of high biological value.

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In vitro evaluation of prebiotic activity, pathogen inhibition and enzymatic metabolism of intestinal bacteria in the presence of fructans extracted from agave: A comparison based on polymerization degree

Cited by 35 publications

References 30 publications

Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Performance Evaluation of Tight Ultrafiltration Membrane Systems at Pilot Scale for Agave Fructans Fractionation and Purification

Micro- and Nanostructures of Agave Fructans to Stabilize Compounds of High Biological Value via Electrohydrodynamic Processing

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