In vitro evaluation of osteoblastic cells on bacterial cellulose modified with multi-walled carbon nanotubes as scaffold for bone regeneration

Gutiérrez-Hernández, José Manuel; Escobar‐García, Diana María; Escalante, Alfredo; Flores, Héctor; González, Francisco; Gatenholm, Paul; Toríz, Guillermo

doi:10.1016/j.msec.2017.02.074

Cited by 88 publications

(43 citation statements)

References 47 publications

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“…Surface topography by (AfM) analysis. Surface roughness is a crucial feature for validating BNC in many electronic devices 49,50 and biomedical implementations 51 . The (AFM) in tapping mode was employed to study the topography of the surface area of 30 ×30 µm for the four BNC products (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

171

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Bacterial nanocellulose (Bnc) has been drawing enormous attention because of its versatile properties. Herein, we shed light on the BNC production by a novel bacterial isolate (MD1) utilizing various agroindustrial wastes. Using 16S rRNA nucleotide sequences, the isolate was identified as Komagataeibacter saccharivorans MD1. For the first time, BNC synthesis by K. saccharivorans MD1 was investigated utilizing wastes of palm date, fig, and sugarcane molasses along with glucose on the Hestrin-Schramm (HS) medium as a control. After incubation for 168 h, the highest BNC yield was perceived on the molasses medium recording 3.9 g/L with an initial concentration of (v/v) 10%. The physicochemical characteristics of the BNC sheets were inspected adopting field-emission scanning electron microscope (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) analysis. The FESEM characterization revealed no impact of the wastes on either fiber diameter or the branching scheme, whereas the AFM depicted a BNC film with minimal roughness was generated using date wastes. Furthermore, a high crystallinity index was estimated by XRD up to 94% for the date wastes-derived BNC, while the FTIR analyses exhibited very similar profiles for all BNC films. Additionally, mechanical characteristics and water holding capacity of the produced Bncs were studied. Our findings substantiated that expensive substrates could be exchanged by agro-industrial wastes for Bnc production conserving its remarkable physical and microstructural properties. In the last decades, bacterial nanocellulose (BNC) has earned increasing global interest because of its remarkable physical and chemical properties, including green processing, low production costs, elevated mechanical properties, hydrophilicity, excellent biocompatibility, and biodegradability 1,2. Certain gram-negative non-pathogenic bacterial genera like Rhizobium, Xanthococcus, Pseudomonas, Azotobacter, Aerobacter, and Alcaligenes were reported to produce nanocellulose extracellularly, but the most common BNC-producing strains belong to the genus Komagataeibacter (formerly Acetobacter or commonly acetic acid bacteria) 1. Bacteria produce the BNC through a process of dual coupled steps: polymerization and crystallization. In the bacterial cytoplasm, glucose residues polymerize to β-1,4 glucan linear chains where they are extracellularly secreted. The developed chains are crystallized to microfibrils, then certain numbers of microfibrils consolidate to materialize highly pure 3D porous network of entangled nanoribbons of 20-60 nm in width 3 .

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Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

171

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“…Besides all aforementioned usages, BNC is used (experimentally) as a scaffold in bone tissue engineering [121][122][123], cartilage regeneration [124], laryngeal medialization [125], as a patch for esophageal defect repair [126], and for closure of ventricular septal defects [127], among many others.…”

Section: In Vivo Scaffoldsmentioning

confidence: 99%

“…Biomedical sector -Further current developments in the BNC field mainly concern novel carrier materials for cell cultivation [122,137,138], encapsulation of cells/immobilization of enzymes [139], coating of medical devices [140], and the production of BNC materials using the well-known oxygen-transparent silicone supports [141,142]. In case of medical implants a lot of results is reported not only in the field of soft tissue implants (see Section In vivo scaffolds) but also regarding materials for bone regeneration [121,143,144].…”

Section: Recent Developments In Bnc Materialsmentioning

confidence: 99%

Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state

et al. 2018

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Nanocelluloses are natural materials with at least one dimension in the nano-scale. They combine important cellulose properties with the features of nanomaterials and open new horizons for materials science and its applications. The field of nanocellulose materials is subdivided into three domains: biotechnologically produced bacterial nanocellulose hydrogels, mechanically delaminated cellulose nanofibers, and hydrolytically extracted cellulose nanocrystals. This review article describes today's state regarding the production, structural details, physicochemical properties, and innovative applications of these nanocelluloses. Promising technical applications including gels/foams, thickeners/stabilizers as well as reinforcing agents have been proposed and research from last five years indicates new potential for groundbreaking innovations in the areas of cosmetic products, wound dressings, drug carriers, medical implants, tissue engineering, food and composites. The current state of worldwide commercialization and the challenge of reducing nanocellulose production costs are also discussed.

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“…Osteoblast adhesion and proliferation has also been enhanced by scaffolds containing bacterial cellulose isolated from Gluconacetobacter xylinus and MWCNTs suggesting their role in bone regeneration. Bacterial cellulose was used in bone TE due to its water retaining capacity, stability, and porosity, and it was modified with MWCNTs to increase the mechanical strength of the scaffolds for bone TE …”

Section: Bone Tementioning

confidence: 99%

“…Bacterial cellulose was used in bone TE due to its water retaining capacity, stability, and porosity, and it was modified with MWCNTs to increase the mechanical strength of the scaffolds for bone TE. 79,80…”

Section: Bone Tementioning

confidence: 99%

Nanomaterials as potential and versatile platform for next generation tissue engineering applications

et al. 2019

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Tissue engineering (TE) is an emerging field where alternate/artificial tissues or organ substitutes are implanted to mimic the functionality of damaged or injured tissues. Earlier efforts were made to develop natural, synthetic, or semisynthetic materials for skin equivalents to treat burns or skin wounds. Nowadays, many more tissues like bone, cardiac, cartilage, heart, liver, cornea, blood vessels, and so forth are being engineered using 3‐D biomaterial constructs or scaffolds that could deliver active molecules such as peptides or growth factors. Nanomaterials (NMs) due to their unique mechanical, electrical, and optical properties possess significant opportunities in TE applications. Traditional TE scaffolds were based on hydrolytically degradable macroporous materials, whereas current approaches emphasize on controlling cell behaviors and tissue formation by nano‐scale topography that closely mimics the natural extracellular matrix. This review article gives a comprehensive outlook of different organ specific NMs which are being used for diversified TE applications. Varieties of NMs are known to serve as biological alternatives to repair or replace a portion or whole of the nonfunctional or damaged tissue. NMs may promote greater amounts of specific interactions stimulated at the cellular level, ultimately leading to more efficient new tissue formation. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2433–2449, 2019.

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In vitro evaluation of osteoblastic cells on bacterial cellulose modified with multi-walled carbon nanotubes as scaffold for bone regeneration

Cited by 88 publications

References 47 publications

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state

Nanomaterials as potential and versatile platform for next generation tissue engineering applications

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