In vitro evaluation of novel polymer-coated magnetic nanoparticles for controlled drug delivery

Abstract

Previously uncharacterized poly(N-isopropylacrylamide-acrylamide-allylamine)-coated magnetic nanoparticles (MNPs) were synthesized using silane-coated MNPs as a template for radical polymerization of N-isopropylacrylamide, acrylamide, and allylamine. Properties of these nanoparticles such as size, biocompatibility, drug loading efficiency, and drug release kinetics were evaluated in vitro for targeted and controlled drug delivery. Spherical core-shell nanoparticles with a diameter of 100 nm showed significantly lower systemic toxicity than did bare MNPs, as well as doxorubicin encapsulation efficiency of 72%, and significantly higher doxorubicin release at 41°C compared with 37°C, demonstrating their temperature sensitivity. Released drugs were also active in destroying prostate cancer cells (JHU31). Furthermore, the nanoparticle uptake by JHU31 cells was dependent on dose and incubation time, reaching saturation at 500 μg/mL and 4 hours, respectively. In addition, magnetic resonance imaging capabilities of the particles were observed using agarose platforms containing cells incubated with nanoparticles. Future work includes investigation of targeting capability and effectiveness of these nanoparticles in vivo using animal models. KeywordsMagnetic nanoparticles; Temperature-responsive polymers; Prostate cancer; Doxorubicin Magnetic nanoparticles (MNPs) coated with temperature-sensitive polymers have been attracting great attention because of their various applications in the fields of biotechnology and medicine. In particular, temperature-sensitive coated MNPs have been used extensively in controlled and targeted drug release systems.1 -4 These nanocomposites are superior to the traditional stimuli-responsive systems such as pH and temperature-sensitive polymers, because

PLGA‐PNIPAM Microspheres Loaded with the Gastrointestinal Nutrient NaB Ameliorate Cardiac Dysfunction by Activating Sirt3 in Acute Myocardial Infarction

“…Additionally, a category of microspheres acts as the scaffolds of the cell, carrying the functional cell to the infarct site for the myocardial infarction treatment,33, 34 such as superparamagnetic microspheres, and the characteristics of these microspheres are porosity and high adsorption capacity, resulting in stable conjugation with the target cell. In addition, a category of microspheres based on a temperature sensitive hydrogel are used in myocardial infarction treatment,35, 36, 37 such as PNIPAM and gelatin, and the characteristic of temperature sensitivity guarantees that these microspheres can avoid the problems of a difficult injection and nonlocation, moreover, the degradation rate of these microspheres is slow, which can contribute to the goal of long‐term and slow drug release. The PLGA‐PNIPAM hydrogel microspheres designed in the present study have the advantages of PLGA and PNIPAM, and which can avoid the disadvantages of low loading efficiency, rapid degradation, and easy movement.…”

“…Briefly, NaB (0.5 mg in 10 mL PLGA solution) was added to a PLGA solution (100 mg in 10 mL dichloromethane), and then this complex solution was emulsified in poly (vinyl alcohol) (2% w/v) to form the PLGA‐NaB microspheres. The PLGA‐NaB microspheres were incorporated with PNIPAM as previously reported 39. Briefly, using N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimidehydrochloride and N ‐hydroxysuccinimide (1:1) to activate the carboxyl functional groups of the PLGA‐NaB microspheres, the NIPAM, and N ′‐methylene double acrylamide were copolymerized on the surface of these microspheres by sodium dodecyl sulfate and N , N ′‐methylenebisacrylamide (BIS) and ammonium persulfate polymerization, forming the PP‐N microspheres.…”

Synthesis, Magneto-structural Properties and Colloidal Stability Studies of Ni0.3Zn0.7Fe2O4 Nanoparticles Coated with Pluronic P123 Block Copolymer for Potential Biomedical Applications

“…Among nanomaterials used in biomedical applications, MNPs are particularly attractive because they have inherent magnetic properties. Thus, they can be remotely manipulated by an external magnetic field thereby serving as vectors for drug targeting (Banerjee and Chen 2007;Rahimi et al 2010) or local heat sources in hyperthermiabased therapy (Jordan et al 1999;Baldi et al 2009). Furthermore, MNPs can be designed to combine therapeutic (hyperthermia and drug delivery) modalities to generate a synergistic effect for cancer treatment-the so-called multifunctional nanoparticles (Kulshrestha et al 2012;Shah et al 2012).…”

<i>In Vitro</i> Studies of NIPAAM-MAA-VP Copolymer-Coated Magnetic Nanoparticles for Controlled Anticancer Drug Release*

“…One of these temperature-sensitive polymer-coated MNPs, poly-(N-isopropylacrylamide) (PNIPAAm)-coated MNPs are given particular attention because of their stimuli (temperature) responsiveness and better drug-loading ability [10,11]. These characteristics are due to amphiphilicity, their large inner volume, capacity for manipulation of permeability, and response to an external temperature stimulus with an on-off mechanism [12].…”