In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

Rasad, Mohammad Syaiful Bahari Abdull; Halim, Ahmad Sukari; Hashim, Kamaruddin; Rashid, Ahmad Hazri Abdul; Yusof, Norimah; Shamsuddin, Shaharum

doi:10.1016/j.carbpol.2009.10.048

Cited by 44 publications

(20 citation statements)

References 53 publications

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“…These chitosans were produced by the Standard and Industrial Research Institute of Malaysia (SIRIM Berhad). Previously, it has been reported that O-C and NO-CMC chitosan derivatives are acceptable because of their mechanical strength and excellent biocompatibility [15]. Our present results produce several noteworthy and quality contributions to increase our understanding of the characterization of NO-CMC and O-C biomaterials and the underlying mechanical principles.…”

Section: Introductionsupporting

confidence: 61%

N,O-Carboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C) : Scaffold Characterization

Periayah

Halim

Gomathysankar

et al. 2014

IJBAS

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Chitosan is of great interest because it is biocompatible, biodegradable and abundant in nature. Accurate characterization of modified chitosan biopolymers is essential to optimize their usage. In our present work, we have tested the physicochemical characterization of 4 different types of chitosan biomaterials, which are classified into N,Ocarboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C). We have employed Fourier Transform Infrared Spectroscopy (FTIR) to analyze the functional groups and Scanning Electron Microscopy (SEM) to examine the scaffold membrane properties of each biomaterial. The FTIR analysis confirmed that a large number of alterations were made towards the NO-CMC group of chitosan. Meanwhile, most of the bands observed in the O-C group can generally be found in the standard model of chitosan. Shifting of the carbonyl group is only noticed in O-C group, which distinguishes both chitosan groups at 1644.20-1633.69 cm -1 peak. The NO-CMC and O-C groups, which have compressive porous structures, are able to support tissue and cell adherence via mechanical strength. Chitosan biopolymers, which vary from different grades and forms, are performing best when their unique properties are optimized. Hence, the study of these structurally modified chitosans and their characterization is very important to correlate their usage and properties in various fields.

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Section: Introductionsupporting

confidence: 61%

N,O-Carboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C) : Scaffold Characterization

Periayah

Halim

Gomathysankar

et al. 2014

IJBAS

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“…High cytocompatibility on human skin cells and on hypertrophic scar cells (Abdull Rasad et al, 2010) …”

Section: Cmc Pastes and Sheetsmentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

471

215

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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“…The IR spectra of chitosan and HE-chitosan were recorded on a Nicolet Nexus-470 Fourier Transform Infrared Spectrometer (Abdull Rasad et al, 2010). Data analysis was carried out on Jwstda-32 (Sashiwa et al, 1990;Shigemasa et al, 1996).…”

Section: Characterization Of He-chitosanmentioning

confidence: 99%

“…It can be found in crustacean shell, cartilage of mollusk, fungi and cell wall of plants. Chitosan is naturally non-toxic (Carreira et al, 2010), non-antigenic and antibacterial (Liu et al, 2004a), and biocompatible and biodegradable (Abdull Rasad et al, 2010;Baldrick, 2010;Muzzarelli, 2010), and has good performance in hemostasis and wound healing (Ishihara et al, 2002;Jayakumar et al, 2011). These properties make chitosan as an absorbable biomaterial widely be applied in medicine, food and agriculture.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of a hydroxyethyl derivative of chitosan and evaluation of its biosafety

Shao

Han

Gao

et al. 2015

J. Ocean Univ. China

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Hydroxyethyl chitosan (HE-chitosan) is a water-soluble derivative of chitosan with many apparent biological properties. For example, it is non-toxic and rapidly biodegradable. Moreover, HE-chitosan has advantages in water-solubility, moisture retention and gelling property due to its hydroxyethyl group. However, the biocompatibility and biodegradability of this multifunctional derivative have rarely been documented although they are critical for its application in biomedical and clinical treatments. The purpose of this work was to evaluate the biosafety of HE-chitosan, and draw important clues for its diverse applications. HE-chitosan was synthesized and characterized its chemical structure with FTIR. Its molecular weight (M W ) was determined by gel permeation chromatography (GPC), and its deacetylation degree (DD) was investigated through potentiometric analysis. The cytotoxicity of HE-chitosan on mouse fibroblast cell L929 was tested. The biocompatibility and biodegradability of HE-chitosan in rat and rabbit were evaluated. The FTIR results indicated that the hydroxyethyl groups were linked to C 6 of chitosan. The GPC analysis confirmed that its Mw was about 90.01 kDa. It was also demonstrated that HE-chitosan had excellent biocompatibility and biodegradability in vivo and had no cytotoxicity on L929. These findings indicated that HE-chitosan can potentially be applied as a biomaterial in tissue engineering, drug delivery, and other biomedical fields.

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In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

Cited by 44 publications

References 53 publications

N,O-Carboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C) : Scaffold Characterization

N,O-Carboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C) : Scaffold Characterization

Chitosan as a starting material for wound healing applications

Synthesis and characterization of a hydroxyethyl derivative of chitosan and evaluation of its biosafety

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