In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B

Abstract

Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensitive fluorescent tracer dye, we aim in this study to investigate leakage at implant-abutment connection of three implant systems having the same prosthetic interface. Materials and Methods. Twenty-one implants (seven Astra Tech, seven Euroteknika, and seven Dentium) with the same prosthetic interface were connected to their original abutments, according to the manufacturers' recommendation. After determination of the inner volume of each implant systems, the kinetic quantification of leakage was evaluated for each group using Rhodamine B (10−2 M). For each group, spectrophotometric analysis was performed to detect leakage with a fluorescence spectrophotometer at 1 h (T0) and 48 h (T1) of incubation time at room temperature. Results. Astra Tech had the highest inner volume (6.8 μL), compared to Dentium (4 μL) and Euroteknika (2.9 μL). At T0 and T1, respectively, the leakage volume and percentage of each system were as follows: Astra Tech 0.043 μL or 1.48% (SD 0.0022), 0.08 μL or 5.56% (SD 0.0074), Euroteknika 0.09 μL or 6.93% (SD 0.0913), 0.21 μL or 20.55% (SD 0.0035), and Dentium 0.07 μL or 4.6% (SD 0.0029), 0.12 μL or 10.47% (SD 0.0072). Conclusion. The tested internal conical implant-abutment connections appear to be unable to prevent leakage. In average, Astra Tech implants showed the highest inner volume and the least leakage.

In VitroEvaluation of Bacterial Leakage at Implant-Abutment Connection: An 11-Degree Morse Taper Compared to a Butt Joint Connection

“…In another study by Aloise et al [ 10 ], bacterial leakage was observed in 2 of 10 implants with internal conical connection. Berberi et al [ 11 ] used internal connection implants in their study and bacterial leakage was confirmed in them. Dibart et al [ 20 ], who reported no leakage, assessed the presence of bacterial leakage for only 72 h. An explanation for this diversity in results may be due to differences in Morse design and tapering degrees in the investigated implant samples.…”

“…Berberi et al [ 11 ] studied the three brands of implant systems with the same 11° conical connection and concluded that the tested connections appear to be unable to prevent leakage. Although the accuracy in fabrication and the precision of fit of the components seem to be an important factor in resistance to leakage, nevertheless, we utilized a CE approved 11° Morse taper connection randomly from available brands in the market.…”

Screw stability of CAD‐CAM titanium and zirconia abutments on different implants: An in vitro study

“…One implant brand with two different implant types, diameters, and wall thickness were evaluated. However, commonly used CC implant's connection has been copied by other commonly used manufacturers 57 . A performance similar to CC implant's may be expected from those implants, which copied CC's connection.…”

“…A performance similar to CC implant's may be expected from those implants, which copied CC's connection. However, future studies are required to compare implants with similar connections as different brands may have differences in implant diameter, taper angle, wall thickness, alloy, inner volume, and insertion torque values 57 . Future studies should also be performed to investigate the screw stability of abutments on implants with different connections.…”

Peri-Implant Health and Peri-implant Bone Stability after Immediate Implant Placement

“…Also important in the design of dental implants is the implant/ abutment connection. Multiple in vitro and clinical investigations have shown that a conical connection in combination with internal platform switching reduces micro movements and thus bacterial contamination by propelling sulcus fluid, which results in marginal bone loss [34,35,50]. With its combination of characteristics, the investigated newly designed implant system seems to meet the requirements for a successful dental implant system.…”