In vitro evaluation of indole-3-carboxaldehyde on Vibrio parahaemolyticus biofilms

Rajalaxmi, Murugan; Devi, Vivekanandham Amsa; Pandian, Shunmugiah Karutha

doi:10.1515/biolog-2016-0043

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…The effect of indole and its derivatives on the biofilm formation by V. parahaemolyticus and V. harveyi were examined using an assay in 96-well polystyrene plates (SPL life science, Pocheon, South Korea), as previously reported ( Rajalaxmi et al, 2016 ). The bacterial cells were grown in mLB and treated with or without indole and its halogenated derivatives for 24 h at 30°C without shaking.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Chloroindoles Against Vibrio parahaemolyticus

et al. 2021

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Vibrio parahaemolyticus is a food-borne pathogen recognized as the prominent cause of seafood-borne gastroenteritis globally, necessitating novel therapeutic strategies. This study examined the antimicrobial and antivirulence properties of indole and 16 halogenated indoles on V. parahaemolyticus. Among them, 4-chloroindole, 7-chloroindole, 4-iodoindole, and 7-iodoindole effectively inhibited planktonic cell growth, biofilm formation, bacterial motility, fimbrial activity, hydrophobicity, protease activity, and indole production. Specifically, 4-chloroindole at 20 μg/mL inhibited more than 80% of biofilm formation with a minimum inhibitory concentration (MIC) of 50 μg/mL against V. parahaemolyticus and Vibrio harveyi. In contrast, 7-chloroindole inhibited biofilm formation without affecting planktonic cell growth with a MIC of 200 μg/mL. Both chlorinated indoles caused visible damage to the cell membrane, and 4-chloroindole at 100 μg/mL had a bactericidal effect on V. parahaemolyticus within 30 min treatment, which is superior to the effect of tetracycline at the same dose. The quantitative structure-activity relationship (QSAR) analyses revealed that chloro and bromo at positions 4 or 5 of the indole are essential for eradicating the growth of V. parahaemolyticus. These results suggest that halogenated indoles have potential use in antimicrobial and antivirulence strategies against Vibrio species.

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Section: Methodsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Chloroindoles Against Vibrio parahaemolyticus

et al. 2021

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“…we speculated that the reduction of swarming motility, which is Rajalaxmi et al, 2016). On the other hand, it has been reported that the presence of higher concentrations of indole increases the biofilm formation of V. cholerae (Nuidate et al, 2016).…”

Section: Discussionmentioning

confidence: 82%

“…Moreover, indole‐3‐carboxaldehyde (ICA) has also been shown to decrease the swarming but promoted the swimming motility of V . parahaemolyticus (Rajalaxmi et al, 2016). In the present study, we demonstrate that 0.25–1 mM indole dose‐dependently decreased swimming (27.55%–58.88%) and also influenced the swarming of VP AHPND in the plate assay.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, it has been understood that motility, biofilm formation and capsule production of V . parahaemolyticus are directly controlled by the QS regulator OpaR (Rajalaxmi et al, 2016). On the other hand, it has been reported that the presence of higher concentrations of indole increases the biofilm formation of V .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that virulence-associated phenotypes of V. parahaemolyticus such as biofilm formation, iron utilization and swarming motility are controlled by QS system (León-Sicairos et al, 2015;Rajalaxmi et al, 2016). Among the signalling molecules, indole is proposed as one of the putative signalling molecules involved in the QS system and has received more attention due to its effects on various physiologies and virulence of many bacteria such as V. cholerae and V. anguillarum (Li et al, 2014;Nuidate et al, 2016).…”

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confidence: 99%

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Indole inhibits quorum sensing‐dependent phenotypes and virulence of acute hepatopancreatic necrosis disease‐causing Vibrio parahaemolyticus

Paopradit

Aksonkird

Mittraparp‐arthorn

2022

Aquaculture Research

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Acute hepatopancreatic necrosis syndrome (AHPND) caused by a unique Vibrio parahaemolyticus strain (VPAHPND) has emerged as a serious disease of cultured shrimps and is causing great economic losses worldwide. In this work, the effects of indole on quorum sensing‐dependent (QS) phenotypes of VPAHPND were assessed. In QS‐inhibitory bioassay using biomonitor strains, indole could interfere with N‐acyl homoserine lactones (AHL)‐mediated violacein pigment production of Chromobacterium violaceum, and autoinducer‐1 (AI‐1) and autoinducer‐2 (AI‐2)‐mediated QS response (bioluminescence) in V. campbellii. Further testing of indole with VPAHPND demonstrated significant decrease in the swimming and swarming motilities. In addition, VPAHPND grown in the presence of indole exhibited a reduction in biofilm formation, extracellular polymeric substances (EPSs) production and autoaggregation index. However, indole did not affect the ability of VPAHPND to use ferrous sulphate (FeSO4) as an iron source. Interestingly, indole reduced the expression of pirA (~2 fold), pirB (~2 fold), cpsA (~1.5 fold) and scrA (~1.7 fold) resulting in the reduction of toxin and capsule production. Lastly, indole‐treated VPAHPND exhibited lower virulence towards an infected model and its specific host, Galleria mellonella larvae and Litopenaeus vannamei respectively. These demonstrated the effects of indole in reducing multiple QS‐dependent phenotypes and virulence of VPAHPND. Thus, using indole, indole‐containing compounds, or indole‐producing organisms might be an attractive anti‐virulence strategy to control infections caused by VPAHPND.

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Motility of Vibrio spp.: regulation and controlling strategies

Khan

Tabassum

Anand

et al. 2020

Appl Microbiol Biotechnol

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In vitro evaluation of indole-3-carboxaldehyde on Vibrio parahaemolyticus biofilms

Abstract: This study was aimed to explore antibiofilm agents from the hitherto underexplored Palk Bay seawater bacteria. The cell free culture supernatant of the isolate

Cited by 9 publications

References 36 publications

Antibacterial and Antibiofilm Activities of Chloroindoles Against Vibrio parahaemolyticus

Antibacterial and Antibiofilm Activities of Chloroindoles Against Vibrio parahaemolyticus

Indole inhibits quorum sensing‐dependent phenotypes and virulence of acute hepatopancreatic necrosis disease‐causing Vibrio parahaemolyticus

Motility of Vibrio spp.: regulation and controlling strategies

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