In Vitro evaluation of immediate cytotoxicity of resterilised orthodontic bands on HGF-1 cell line

Ajami, Shabnam; Dadras, Saeideh; Faghih, Zahra; Shobeiri, Saeideh Sadat; Mahdian, Arezoo

doi:10.1016/j.ortho.2021.04.006

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…The cell viability was conducted using MTT (Sigma–Aldrich, St. Louis, MO, USA), and the absorbance (OD value) was measured at 492 nm. Then, the cell viability value was calculated on the basis of the following formula, as previously used [ 30 ].

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Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Immortal Human Keratinocyte HaCaT Cell Line Damaged by Tritiated Water

Zhang

Zhou

et al. 2023

Biology

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Radioactive elements, such as tritium, have been released into the ocean in large quantities as a result of the reactor leakage accident. In this study, an MTT assay demonstrated that the viability of HacaT cells decreased after tritiated water treatment. Bioinformatics analysis was used to analyze gene changes in the HacaT cells. The sequencing results showed 267 significantly differentially expressed genes (DEGs), and GO enrichment analysis showed that the DEGs were mainly divided into three parts. The KEGG pathway analysis showed that the up-regulated DEGs were involved in Wnt and other pathways, while the down-regulated DEGs were involved in Jak–STAT and others. A Western blot assay was used to verify the parts of the sequencing results. This study was the first to explore the mechanism of tritiated water on HacaT cells using Transcriptome analysis. The results will provide a theoretical basis for the study of tritiated water hazard mechanisms.

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…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Immortal Human Keratinocyte HaCaT Cell Line Damaged by Tritiated Water

Zhang

Zhou

et al. 2023

Biology

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“…[ 3 ] Previous studies have demonstrated negligible levels of cytotoxicity for various orthodontic appliances. [ 4 5 ] However, metal ions in direct contact with the oral mucosa, while simultaneously being mechanically irritated by orthodontic appliances, have the potential to induce cellular damage in oral mucosa cells, even if they do not reach hazardous levels in susceptible individuals. [ 6 ] Nickel is a strong immunologic sensitizer, while chromium ions leached from metal alloys negatively affect some enzyme activity, oxygen consumption, and intracellular adenosine triphosphate levels and are considered mutagenic.…”

Section: Introductionmentioning

confidence: 99%

Metal release and cytotoxicity of different orthodontic bracket-wire combinations: An in vitro study

Aiswareya,

Verma,

Khan

et al. 2023

JISPCD

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Aim: To quantify and compare the metal ions released from different bracket-wire combinations and to assess their cytotoxicity. Materials and Methods: A total of 360 fabricated sectional fixed orthodontic appliances were divided into 6 groups. The first three groups consisted of stainless-steel brackets with stainless-steel, snickel-titanium (NiTi), and titanium-molybdenum alloy (TMA) archwires, and the other three groups were fabricated using ceramic brackets (polycrystalline alumina) with stainless-steel, NiTi, and TMA archwires. These appliances were immersed in artificial saliva (pH 6.5 ± 0.5, 37°C), for 1 week, 2 weeks, and 1 month. The nickel and chromium ions released in the artificial saliva were quantified using a flame atomic absorption spectrometer, and cytotoxicity assessment was performed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on human cervical cancer cell line. Results: The stainless-steel bracket groups displayed a significantly greater release of nickel and chromium ions compared to the ceramic bracket groups (P < 0.05). However, no significant differences were identified when comparing the three archwire types within the stainless-steel/ceramic bracket groups. At the end of 1 month, the % cell viability demonstrated by the appliances was in the decreasing order of stainless-steel-TMA > ceramic-stainless steel > stainless-steel-NiTi > ceramic-NiTi > stainless-steel-stainless steel > ceramic-TMA. Conclusion: Considerably greater release of nickel and chromium ions was observed from the appliances utilizing stainless-steel brackets compared to those employing ceramic brackets. However, no remarkable difference in the levels of nickel and chromium ions was observed when comparing the three archwires: stainless steel, NiTi, and TMA. In the cytotoxicity assessment, the ceramic-TMA combination displayed the highest level of cytotoxicity, while the stainless-steel-TMA combination displayed the least cytotoxicity.

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“…Even if the device itself does not cause serious damage to the oral cavity, it may make it more susceptible to damage and toxic substances later in life [ 1 , 7 , 8 ]. Although studies on the cytotoxicity of these metals and orthodontic appliances have been conducted frequently and most show that cytotoxicity is negligible, it cannot be ruled out that low concentrations of metals are sufficient to cause biological changes in the oral mucosa, especially because they are in the form of mixtures [ 1 , 9 , 10 ]. The mucosal tissue of the oral cavity can absorb these low levels of metal ions over a long period of time, leading to changes in the genome of the cells of the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, fibroblast cell lines (periodontal ligament, gingival, or dermal [ 9 , 10 , 11 , 12 , 13 , 14 ]), gastrointestinal tract cell lines [ 15 , 16 ], and sometimes yeast [ 17 , 18 ] are most commonly used for testing. Research has focused mainly on NiTi or stainless (arch)wires [ 2 , 13 , 19 , 20 ], brackets [ 2 , 12 , 14 ], and bands [ 2 , 9 , 10 , 16 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Metal Ions Released from a Fixed Orthodontic Appliance to Gastrointestinal Tract Cell Lines

Durgo

Orešić

Mlinarić³

et al. 2023

IJMS

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The mechanism of toxicity and cellular response to metal ions present in the environment is still a very current area of research. In this work, which is a continuation of the study of the toxicity of metal ions released by fixed orthodontic appliances, eluates of archwires, brackets, ligatures, and bands are used to test the prooxidant effect, cytotoxicity, and genotoxicity on cell lines of the gastrointestinal tract. Eluates obtained after three immersion periods (3, 7, and 14 days) and with known amounts and types of metal ions were used. Four cell lines—CAL 27 (human tongue), Hep-G2 (liver), AGS (stomach) and CaCo-2 (colon)—were treated with each type of eluate at four concentrations (0.1×, 0.5×, 1.0×, and 2.0×) for 24 h. Most eluates had toxic effects on CAL 27 cells over the entire concentration range regardless of exposure time, while CaCo-2 proved to be the most resistant. In AGS and Hep-G2 cells, all samples tested induced free radical formation, with the highest concentration (2×) causing a decrease in free radicals formed compared to the lowest concentrations. Eluates containing Cr, Mn, and Al showed a slight pro-oxidant effect on DNA (on plasmid φX-174 RF I) and slight genotoxicity (comet assay), but these effects are not so great that the human body could not “resist” them. Statistical analysis of data on chemical composition, cytotoxicity, ROS, genotoxicity, and prooxidative DNA damage shows the influence of metal ions present in some eluates on the toxicity obtained. Fe and Ni are responsible for the production of ROS, while Mn and Cr have a great influence on hydroxyl radicals, which cause single-strand breaks in supercoiled plasmid DNA in addition to the production of ROS. On the other hand, Fe, Cr, Mn, and Al are responsible for the cytotoxic effect of the studied eluates. The obtained results confirm that this type of research is useful and brings us closer to more accurate in vivo conditions.

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In Vitro evaluation of immediate cytotoxicity of resterilised orthodontic bands on HGF-1 cell line

Cited by 3 publications

References 22 publications

Transcriptome Analysis of the Immortal Human Keratinocyte HaCaT Cell Line Damaged by Tritiated Water

Transcriptome Analysis of the Immortal Human Keratinocyte HaCaT Cell Line Damaged by Tritiated Water

Metal release and cytotoxicity of different orthodontic bracket-wire combinations: An in vitro study

Toxicity of Metal Ions Released from a Fixed Orthodontic Appliance to Gastrointestinal Tract Cell Lines

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