In Vitro Evaluation of Hyperthermia Magnetic Technique Indicating the Best Strategy for Internalization of Magnetic Nanoparticles Applied in Glioblastoma Tumor Cells

Mamani, Javier Bustamante; Souza, Taylla Klei Felix; Nucci, Mariana Penteado; Oliveira, Fernando A.; Nucci, Leopoldo Penteado; Alves, Arielly H.; Rego, Gabriel N. A.; Marti, Luciana Cavalheiro; Gamarra, Lionel Fernel

doi:10.3390/pharmaceutics13081219

Cited by 13 publications

(9 citation statements)

References 79 publications

(94 reference statements)

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“…The characterization of MFNP dispersed in culture media confirmed a good MFNP colloidal stability over 18 h, retaining the hydrodynamic diameter of 58 nm, an important aspect of the cell labeling process [13,53,54]. The imbalance of forces involved in the interaction of nanoparticles with the medium can lead them to agglomerate [35,55] and thus to their non-internalization and toxicity for hBM-MSC.…”

Section: Discussionmentioning

confidence: 75%

“…There are certain actions that can improve nanoparticle internalization in cells, such as increasing the period of incubation, surface modification, transfection agents (Poly-D-Lysine and protamine sulfate), and application of magnetic field already evaluated in previous studies [33][34][35][36][37]. The magnetic field attracts the nanoparticle, which contributes to cellular internalization throughout the labeling process [38].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies

et al. 2022

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Considering there are several difficulties and limitations in labeling stem cells using multifunctional nanoparticles (MFNP), the purpose of this study was to determine the optimal conditions for labeling human bone marrow mesenchymal stem cells (hBM-MSC), aiming to monitor these cells in vivo. Thus, this study provides information on hBM-MSC direct labeling using multimodal nanoparticles in terms of concentration, magnetic field, and period of incubation while maintaining these cells’ viability and the homing ability for in vivo experiments. The cell labeling process was assessed using 10, 30, and 50 µg Fe/mL of MFNP, with periods of incubation ranging from 4 to 24 h, with or without a magnetic field, using optical microscopy, near-infrared fluorescence (NIRF), and inductively coupled plasma mass spectrometry (ICP-MS). After the determination of optimal labeling conditions, these cells were applied in vivo 24 h after stroke induction, intending to evaluate cell homing and improve NIRF signal detection. In the presence of a magnetic field and utilizing the maximal concentration of MFNP during cell labeling, the iron load assessed by NIRF and ICP-MS was four times higher than what was achieved before. In addition, considering cell viability higher than 98%, the recommended incubation time was 9 h, which corresponded to a 25.4 pg Fe/cell iron load (86% of the iron load internalized in 24 h). The optimization of cellular labeling for application in the in vivo study promoted an increase in the NIRF signal by 215% at 1 h and 201% at 7 h due to the use of a magnetized field during the cellular labeling process. In the case of BLI, the signal does not depend on cell labeling showing no significant differences between unlabeled or labeled cells (with or without a magnetic field). Therefore, the in vitro cellular optimized labeling process using magnetic fields resulted in a shorter period of incubation with efficient iron load internalization using higher MFNP concentration (50 μgFe/mL), leading to significant improvement in cell detection by NIRF technique without compromising cellular viability in the stroke model.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies

et al. 2022

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“…However, before evaluating HSPC biodistribution by ICP-MS, the SPION internalization was confirmed by brightfield microscopy image after Prussian blue staining, in which the internalized nanoparticles are highlighted in the blue color, resulting from the formation of the Fe 4 [Fe(CN) 6 ] 3 complex. This method is widely used in a range of cells, from undifferentiated cells, such as stem and progenitor cells [ 38 , 39 ] to tumor cells [ 40 ], and also reported in HSPC [ 37 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation

et al. 2023

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Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 µg Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 ± 0.37 µg Fe/mL in the control and 6.61 ± 0.84 µg Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 ± 0.66 mg Fe/g in the spleen of the control group and 2.17 ± 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness.

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“…Nanoparticle cellular uptake and the mechanism of cell death due to hyperthermia are of paramount importance in explaining their eventual clinical applications [ 18 , 19 ]. Mamani et al reported an in vitro study that evaluated the magnetic hyperthermia technique for the internalization of amino saline functionalized ferrite nanoparticles to target glioblastoma tumor cells in static and dynamic magnetic fields [ 20 ]. They were able to achieve up to 70% cell death when exposed for 40 min to an AMF (with a frequency of 557 kHz and an intensity of 300 Gauss).…”

Section: Introductionmentioning

confidence: 99%

Hyperthermia of Magnetically Soft-Soft Core-Shell Ferrite Nanoparticles

Narayanaswamy

Jagal

Khurshid

et al. 2022

IJMS

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Magnetically soft-soft MnFe2O4-Fe3O4 core-shell nanoparticles were synthesized through a seed-mediated method using the organometallic decomposition of metal acetyl acetonates. Two sets of core-shell nanoparticles (S1 and S2) of similar core sizes of 5.0 nm and different shell thicknesses (4.1 nm for S1 and 5.7 nm for S2) were obtained by changing the number of nucleating sites. Magnetic measurements were conducted on the nanoparticles at low and room temperatures to study the shell thickness and temperature dependence of the magnetic properties. Interestingly, both core-shell nanoparticles showed similar saturation magnetization, revealing the ineffective role of the shell thickness. In addition, the coercivity in both samples displayed similar temperature dependencies and magnitudes. Signatures of spin glass (SG) like behavior were observed from the field-cooled temperature-dependent magnetization measurements. It was suggested to be due to interface spin freezing. We observed a slight and non-monotonic temperature-dependent exchange bias in both samples with slightly higher values for S2. The effective magnetic anisotropy constant was calculated to be slightly larger in S2 than that in S1. The magnetothermal efficiency of the chitosan-coated nanoparticles was determined by measuring the specific absorption rate (SAR) under an alternating magnetic field (AMF) at 200–350 G field strengths and frequencies (495.25–167.30 kHz). The S2 nanoparticles displayed larger SAR values than the S1 nanoparticles at all field parameters. A maximum SAR value of 356.5 W/g was obtained for S2 at 495.25 kHz and 350 G for the 1 mg/mL nanoparticle concentration of ferrogel. We attributed this behavior to the larger interface SG regions in S2, which mediated the interaction between the core and shell and thus provided indirect exchange coupling between the core and shell phases. The SAR values of the core-shell nanoparticles roughly agreed with the predictions of the linear response theory. The concentration of the nanoparticles was found to affect heat conversion to a great extent. The in vitro treatment of the MDA-MB-231 human breast cancer cell line and HT-29 human colorectal cancer cell was conducted at selected frequencies and field strengths to evaluate the efficiency of the nanoparticles in killing cancer cells. The cellular cytotoxicity was estimated using flow cytometry and an MTT assay at 0 and 24 h after treatment with the AMF. The cells subjected to a 45 min treatment of the AMF (384.50 kHz and 350 G) showed a remarkable decrease in cell viability. The enhanced SAR values of the core-shell nanoparticles compared to the seeds with the most enhancement in S2 is an indication of the potential for tailoring nanoparticle structures and hence their magnetic properties for effective heat generation.

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In Vitro Evaluation of Hyperthermia Magnetic Technique Indicating the Best Strategy for Internalization of Magnetic Nanoparticles Applied in Glioblastoma Tumor Cells

Cited by 13 publications

References 79 publications

Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies

Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies

Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation

Hyperthermia of Magnetically Soft-Soft Core-Shell Ferrite Nanoparticles

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