In vitro evaluation of hydroxycinnamoyl CoA:quinate hydroxycinnamoyl transferase expression and regulation in Taraxacum antungense in relation to 5-caffeoylquinic acid production

Li, Qun; Yao, Lixiang; Xu, Yachen; Cheng, Huang; Wang, Wei‐Ting; Liu, Zijia; Liu, Jia; Cui, Xili; Zhou, Yu‐Fu; Wei, Ning

doi:10.1016/j.phytochem.2019.02.014

Cited by 17 publications

(21 citation statements)

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“…The CGA and CA levels in T. mongolicum were generally lower than those in T. antungense , while luteolin levels were significantly higher 4 . The possible reasons are various factors, including growth environments, sample extraction, and HPLC methods, which together cause significant differences in CGA, CA, rutin, and luteolin concentrations in T. antungense and closely related species 4 , 21 , 37 . The relationship among the four compounds and total polyphenol concentrations should be further studied.…”

Section: Discussionmentioning

confidence: 99%

“…First, the HQT2 gene promoter was used as bait to screen functional TFs. CGA is widely distributed in many plants, such as T. antungense , L. japonic a, Solanum lycopersicum , and Solanum tuberosum 20 , 21 , 38 . In the CGA biosynthesis pathway, key enzyme genes, such as PAL, C4H, 4CL, and HCT/HQT, are widely reported 14 , 20 .…”

Section: Discussionmentioning

confidence: 99%

“…CA is directly catalytically synthesized by coumarate 3-hydroxylase from p-coumaroyl-CoA, whereas CGA is catalytically synthesized by hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase (HQT) and hydroxycinnamoyl-CoA shiki-mate/quinate hydroxycinnamoyl transferase (HCT) 20 . However, in T. antungense , only HQTs ( TaHQT1 / 2 ) have been isolated, identified, and assessed, and TaHQT2 is the last rate-limiting enzyme in chlorogenic acid biosynthesis 21 . For flavonoids, p-coumaroyl-CoA is a branch and precursor compound.…”

Section: Introductionmentioning

confidence: 99%

“…Plant polyphenolic compounds are important components acting against biotic and abiotic stresses 21 , 25 . Plant transcription factor (TF) family members, such as MYB11/12/111 and WRKY18/40/60, respond to biotic and abiotic stress to regulate the expression of downstream structural genes and ultimately promote the biosynthesis of polyphenols 18 , 19 , 27 .…”

Section: Introductionmentioning

confidence: 99%

“…Coexpression analysis of TFs and biosynthesis pathway gene expression levels often showed a highly linear relationship 27 , 28 . Thus, TFs may be used as a tool not only to improve a plant’s ability to adapt to the environment but also to increase polyphenolic compound production in plants 21 .…”

Section: Introductionmentioning

confidence: 99%

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The basic helix-loop-helix transcription factor TabHLH1 increases chlorogenic acid and luteolin biosynthesis in Taraxacum antungense Kitag

Liu

Cheng

et al. 2021

Hortic Res

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Polyphenols are the main active components of the anti-inflammatory compounds in dandelion, and chlorogenic acid (CGA) is one of the primary polyphenols. However, the molecular mechanism underlying the transcriptional regulation of CGA biosynthesis remains unclear. Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase (HQT2) is the last rate-limiting enzyme in chlorogenic acid biosynthesis in Taraxacum antungense. Therefore, using the TaHQT2 gene promoter as a probe, a yeast one-hybrid library was performed, and a basic helix-loop-helix (bHLH) transcription factor, TabHLH1, was identified that shared substantial homology with Gynura bicolor DC bHLH1. The TabHLH1 transcript was highly induced by salt stress, and the TabHLH1 protein was localized in the nucleus. CGA and luteolin concentrations in TabHLH1-overexpression transgenic lines were significantly higher than those in the wild type, while CGA and luteolin concentrations in TabHLH1-RNA interference (RNAi) transgenic lines were significantly lower. Quantitative real-time polymerase chain reaction demonstrated that overexpression and RNAi of TabHLH1 in T. antungense significantly affected CGA and luteolin concentrations by upregulating or downregulating CGA and luteolin biosynthesis pathway genes, especially TaHQT2, 4-coumarate-CoA ligase (Ta4CL), chalcone isomerase (TaCHI), and flavonoid-3′-hydroxylase (TaF3′H). Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays indicated that TabHLH1 directly bound to the bHLH-binding motifs of proTaHQT2 and proTa4CL. This study suggests that TabHLH1 participates in the regulatory network of CGA and luteolin biosynthesis in T. antungense and might be useful for metabolic engineering to promote plant polyphenol biosynthesis.

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Section: Discussionmentioning

confidence: 99%