In vitro EVALUATION OF EUCALYPTUS ECTOMYCORRHIZAE ON SUBSTRATE WITH PHOSPHORUS DOSES FOR FUNGAL PRE-SELECTION

Costa, Lidiomar Soares da; Grazziotti, Paulo Henrique; Grazziotti, Danielle Cristina Fonseca Santos; Silva, Alexandre Christófaro; Rossi, Márcio José; Silva, Enilson de Barros; Costa, Victor Hugo Duarte da; Gomes, Ângela Laís Fernandes

doi:10.1590/0100-67622015000100012

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…On the other hand, concentrations above 3% resulted in signi cant viscosity, preventing the solution from forming beads properly. The most commonly used concentration is 2%, as observed in other studies involving the entrapment of ECM mycelia(Rodrigues et al 1999;Oliveira et al 2006;Repáč 2007;Rossi et al 2017;Costa et al 2019). In this study, 2% sodium alginate was the optimal concentration, providing the highest viability of A. odoratus mycelia and proper bead formation.…”

mentioning

confidence: 55%

Optimizing conditions of mycelial inoculum immobilized in Ca-alginate beads: a case study in ectomycorrhizal fungus Astraeus odoratus

Punsung,

Pachit,

Kijpornyongpan

et al. 2024

World J Microbiol Biotechnol

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mentioning

confidence: 55%

Optimizing conditions of mycelial inoculum immobilized in Ca-alginate beads: a case study in ectomycorrhizal fungus Astraeus odoratus

Punsung,

Pachit,

Kijpornyongpan

et al. 2024

World J Microbiol Biotechnol

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“…The above-mentioned results suggested that diverse factors, such as ECM-fungus species or isolate, host species, and growth conditions, could influence plant Al tolerance following ECM inoculation. Bucking et al (40) suggested that mycorrhizae altered the host and fungus function, while Costa et al (41) observed that Pisolithus inoculation enhanced the growth of Eucalyptus urophylla. Overall, among the three tested ECM isolates, L. bicolor S238A was the most promising isolate, considering its capacity to confer Al tolerance in P. massoniana seedlings grown in an acidic yellow soil.…”

Section: Discussionmentioning

confidence: 99%

Laccaria bicolor Mobilizes both Labile Aluminum and Inorganic Phosphate in Rhizosphere Soil of Pinus massoniana Seedlings Field Grown in a Yellow Acidic Soil

Wang

et al. 2020

Appl Environ Microbiol

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Plant growth is often limited by highly activated aluminum (Al) and low available phosphorus (P) in acidic soil. Ectomycorrhizal (ECM) fungi can improve their host plants’ Al tolerance by increasing P availability while decreasing Al activity in vitro or in hydroponic or sand culture systems. However, the effect of ECM fungi on inorganic P (IP) and labile Al in acidic soil in the field, particularly in conjunction with Al treatment, remains poorly understood. The present study aimed to determine the influence of ECM fungal association on the mobilization of IP and labile Al in rhizosphere soil of host plants grown in the field with external Al treatment and the underlying nutritional mechanism in plant Al tolerance. To do so, 4-week-old Pinus massoniana seedlings were inoculated with three ECM isolates (Laccaria bicolor 270, L. bicolor S238A, and L. bicolor S238N) and grown in a Haplic Alisol field with or without Al treatment for 12 weeks. Results showed that L. bicolor association enhanced the available P depletion and facilitated the mobilization of IP and labile Al, in turn improving the capacity of host plant to use Al-bound P, Ca-bound P, and occluded P, particularly when P. massoniana seedlings were inoculated with L. bicolor S238A. Inoculation with L. bicolor isolates also enhanced the solubility of labile Al and facilitated the conversion of acid-soluble Al into exchangeable Al. Our findings suggested that ECM inoculation could enhance plant Al tolerance in the field by mobilizing IP to improve the P bioavailability but not by decreasing Al activity. IMPORTANCE Here, we reveal the underlying nutritional mechanism in plant Al tolerance conferred by ectomycorrhizal (ECM)-fungus inoculation in the field and report the screening of a promising ECM isolate to assist phytoremediation and afforestation using Pinus massoniana in acidic soil in southern China. This study advances our understanding of the contribution of ECM fungi to plant–ECM-fungus symbiosis and highlights the vital role of ECM-fungus inoculation in plant Al tolerance. In addition, the results described in the present study confirm the importance of carrying out studies in the field rather than only in vitro studies. Our findings strengthen our understanding of the role of ECM-fungus association in detecting, utilizing, and transporting unavailable nutrients in the soil to enhance host plant growth and adaptability in response to adverse habitats.

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“…These results suggested that Al resistance in ECM plants was affected by numerous factors, including ECM fungus species or isolates, host plant species, and growth conditions. Bucking et al [25] suggested that ectomycorrhizae could alter the function of both the host and fungus, while Costa et al [26] found that the growth of Eucalyptus urophylla benefited from an association with Pisolithus sp. Our results indicated that the symbiosis benefited the growth and function of both L. bicolor S238A and P. massoniana seedlings, but the underlying mechanisms need further study.…”

Section: Aluminum Resistance and Biomass Production In Ecm P Massonimentioning

confidence: 99%

Accumulation and Translocation of Phosphorus, Calcium, Magnesium, and Aluminum in Pinus massoniana Lamb. Seedlings Inoculated with Laccaria bicolor Growing in an Acidic Yellow Soil

Wang

et al. 2019

Forests

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Research Highlights: We demonstrate that ectomycorrhizal (ECM) fungi improve plant aluminum (Al)-tolerance in the field and Laccaria bicolor S238A is a promising ECM isolate. Furthermore, we interpret the underlying nutritional mechanism that ECM inoculation facilitates aboveground biomass production as well as nutrients accumulation and translocation. Background and Objectives: Al toxicity is a primary limiting factor for plants growing in acidic soils. Hydroponic/sand culture studies have shown that some ECM fungi could enhance plant Al-tolerance. However, the underlying mechanisms of ECM fungi in improving plant Al-tolerance in the field are still unknown. To fill this knowledge gap, the present study aimed to examine roles of ECM inoculation in biomass production, accumulation and translocation of nutrients and Al in the host plant grown in the field under Al treatment. Materials and Methods: 4-week-old Pinus massoniana seedlings were inoculated with three Laccaria bicolor isolates (L. bicolor 270, L. bicolor S238A or L. bicolor S238N) and grown in an acidic yellow soil under 1.0 mM Al treatment for 12 weeks in the field. Biomass production, accumulation and translocation of P, Ca, Mg, and Al were investigated in these 16-week-old P. massoniana seedlings. Results: All three of these L. bicolor isolates improved biomass production as well as P, Ca and Mg accumulation in P. massoniana seedlings. Moreover, the three ECM isolates facilitated the translocation of P, Ca, and Mg to aboveground in response to Al treatment, particularly when seedlings were inoculated with L. bicolor S238A. In addition, both L. bicolor 270 and L. bicolor S238A had no apparent effects on Al accumulation, while enhanced Al translocation to aboveground. In contrast, L. bicolor S238N decreased Al accumulation but had no significant effect on Al translocation. Conclusions: ECM fungi in the field improved plant Al-resistance by increasing nutrient uptake, and this was mostly due to translocation of P, Ca, and Mg to aboveground, not by decreasing the uptake and translocation of Al.

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In vitro EVALUATION OF EUCALYPTUS ECTOMYCORRHIZAE ON SUBSTRATE WITH PHOSPHORUS DOSES FOR FUNGAL PRE-SELECTION

Cited by 6 publications

References 17 publications

Optimizing conditions of mycelial inoculum immobilized in Ca-alginate beads: a case study in ectomycorrhizal fungus Astraeus odoratus

Optimizing conditions of mycelial inoculum immobilized in Ca-alginate beads: a case study in ectomycorrhizal fungus Astraeus odoratus

Laccaria bicolor Mobilizes both Labile Aluminum and Inorganic Phosphate in Rhizosphere Soil of Pinus massoniana Seedlings Field Grown in a Yellow Acidic Soil

Accumulation and Translocation of Phosphorus, Calcium, Magnesium, and Aluminum in Pinus massoniana Lamb. Seedlings Inoculated with Laccaria bicolor Growing in an Acidic Yellow Soil

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