In-vitro evaluation of effects of intracanal medicaments on viability, proliferation, and differentiation of stem cells from apical papilla - A systematic review

Rahul, Morankar

doi:10.14744/eej.2022.63835

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…A review of in-vitro studies concluded that CH is preferred over antibiotic pastes prior to REP in regards of viability, proliferation, and differentiation of MSCs. 17 Thus, we investigated the effects of CH and NCH used as ICM during REP on the viability, and hard tissue differentiation potential of hPDLSCs. Exploring such effects is important because they are assumed to be involved in periodontal regeneration and healing of apical periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Saber

2024

Ain Shams Dental Journal

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To evaluate the effect of CH and NCH on the viability, attachment, osteogenic, cementogenic and dentinogenic differentiation potential of hPDLSCs in-vitro. Materials and Methods: Calcium Hydroxide (CH) and nano calcium hydroxide (NCH) powders were initially characterized using transmission electron microscope (TEM), X-ray Diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and a zeta sizer instrument. Standard dentin discs were prepared, irrigated according to the Regenerative Endodontic Procedures (REP) protocol, then treated with 1 mg/ml of CH or NCH pastes, and incubated for 1 week. Untreated discs served as control. Then, they were washed off, and all discs were treated with 17% EDTA before seeding of human periodontal ligament stem cells (hPDLSCs) . Cell viability was assessed using MTT assay. Mineralized nodules and alkaline phosphatase (ALP) activity were determined to assess cell differentiation. Cell dentino/cementogenic and osteogenic potential were assessed through monitoring gene expression levels Dentin sialophosphoprotein (DSSP), Cementum protein 1 (CEMP1), Runt-related transcription factor 2 (RUNX2), Osteoprotegrin (OG), and Osteocalcin (OC) using RT-qPCR. Results: TEM examination of NCH powder particles revealed a regular pattern and hexagonal shape with side dimension up to 60 ±10 nm. XRD analysis of NCH powder demonstrated its highly crystalline nature. FTIR spectroscopy confirmed that CH and NCH consisted mainly of pure calcium hydroxide. The zeta sizer instrument showed that NCH is positively charged with an average zeta potential of +27.8 mV. hPDLSCs seeded on NCH-treated discs showed significantly higher cell survival , more mineralization, higher alkaline phosphatase activity and increased expression of DSPP, CEMP1, RUNX2, OPG and OC markers. Conclusion: NCH can be an alternative ICM during REP.

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Section: Discussionmentioning

confidence: 99%

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Saber

2024

Ain Shams Dental Journal

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“…However, their potential side effects and damage to the resident stem cells remain of concern. A recent systematic review evaluating the in vitro cytotoxicity of TAP, DAP, and calcium hydroxide on SCAP concluded that the use of antibiotic pastes lead to a reduction in SCAP survival compared to calcium hydroxide, although distinctions could not be made between the antibiotic pastes [20]. Other potential complications of antibiotic pastes are the development of resistant organisms, interference with the release of dentinal growth factors, inhibition of vascularization, and high toxicity levels [12].…”

Section: Discussionmentioning

confidence: 99%

“…Given the heterogeneity in the reported properties of common intracanal medicaments [20], the search for a biocompatible intracanal medicament with good antimicrobial properties is ongoing. 2-Hydroxyisocaproic acid (HICA) is a natural metabolite in the leucine degradation pathway in humans and certain Lactobaccilus species.…”

Section: Introductionmentioning

confidence: 99%

A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP Differentiation

Smoczer,

Park,

Herrington

et al. 2023

Dentistry Journal

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Intracanal medicaments with maximal antimicrobial efficacy and minimal damage to resident stem cells are essential for successful regenerative endodontic procedures. 2-Hydroxyisocaproic acid (HICA) could have the attributes of a potential intracanal medicament. This study evaluates its cytotoxicity, genotoxicity, and effects on the odontogenic and osteogenic differentiation of the stem cells of the apical papilla (SCAP). Cytotoxicity and cell viability assays were performed on cells treated for 24, 48, and 72 h with varying concentrations of HICA and compared to the standard intracanal medicament, calcium hydroxide. The genotoxicity was assessed via immunofluorescence for two markers of DNA double-strand breaks: phosphorylated γH2AX and 53BP1. The SCAP differentiation was evaluated based on the alkaline phosphatase activity, Alizarin Red staining, and expression of odontogenic and osteogenic genes (DSPP1, BSP1, OCN, RUNX2) in the presence of selected HICA concentrations. HICA was not cytotoxic at concentrations up to 10 mg/mL, regardless of the exposure time, although it was cytostatic at all tested concentrations. HICA was not genotoxic at concentrations below 5 mg/mL. No difference in cytotoxicity or genotoxicity was found between HICA and calcium hydroxide at 1 mg/mL. HICA retained about 70% of the osteogenic differentiation potential at 1 mg/mL. Within the limitations of this in vitro study, we show that HICA at 1 mg/mL could be a potential intracanal medicament for REPs.

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In-vitro evaluation of effects of intracanal medicaments on viability, proliferation, and differentiation of stem cells from apical papilla - A systematic review

Cited by 2 publications

References 42 publications

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP Differentiation

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