In vitro evaluation of different remineralization periods in improving the resistance of previously eroded bovine dentine against tooth-brushing abrasion

Attin, Thomas; Buchalla, Wolfgang; Pütz, Barbara

doi:10.1016/s0003-9969(01)00039-5

Cited by 41 publications

(57 citation statements)

References 15 publications

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“…Various other studies concerning dentine erosion have used bovine dentine 14,21,22 . An advantage of using bovine dentine is that it is easier to obtain a sufficient number of sound bovine teeth instead of human teeth 23 .…”

Section: Discussionmentioning

confidence: 99%

Application of cerium chloride to improve the acid resistance of dentine

Wegehaupt

Sener

Attin

et al. 2010

Archives of Oral Biology

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R (2010). Application of cerium chloride to improve the acid resistance of dentine. Archives of Oral Biology, 55(6): [441][442][443][444][445][446] Application of cerium chloride to improve the acid resistance of dentine Abstract OBJECTIVE: To investigate the effect of cerium chloride, cerium chloride/fluoride and fluoride application on calcium release during erosion of treated dentine. METHODS: Forty dentine samples were prepared from human premolars and randomly assigned to four groups (1-4). Samples were treated twice a day for 5 days, 30s each, with the following solutions: group 1 placebo, group 2 fluoride (Elmex fluid), group 3 cerium chloride and group 4 combined fluoride and cerium chloride. For the determination of acid resistance, the samples were consecutively eroded six times for 5 min with lactic acid (pH 3.0) and the calcium release in the acid was determined. Furthermore, six additional samples per group were prepared and used for EDS analysis. SEM pictures of these samples of each group were also captured. RESULTS: Samples of group 1 presented the highest calcium release when compared with the samples of groups 2-4. The highest acid resistance was observed for group 2. Calcium release in group 3 was similar to that of group 4 for the first two erosive attacks, after which calcium release in group 4 was lower than that of group 3. Generally, the SEM pictures showed a surface coating for groups 2-4. No deposits were observed in group 1. CONCLUSION: Although fluoride showed the best protective effect, cerium chloride was also able to reduce the acid susceptibility of dentine significantly, which merits further investigation. Key words: dentine, fluoride, cerium chloride, acid, erosion Application of cerium chloride to improve the acid resistance of dentine Abstract:Objective: To investigate the effect of cerium chloride, cerium chloride/fluoride and fluoride application on calcium release during erosion of treated dentine.Methods: Forty dentine samples were prepared from human premolars and randomly assigned to four groups (1 -4). Samples were treated twice a day for 5 days, 30s each, with the following solutions: group 1 placebo, group 2 fluoride (Elmex fluid), groups 3 cerium chloride and group 4 combined fluoride and cerium chloride. For the determination of acid resistance, the samples were consecutively eroded six times for 5 min with lactic acid (pH 3.0) and the calcium release in the acid was determined.Furthermore, six additional samples per group were prepared and used for EDS analysis. SEM pictures of these samples of each group were also captured.

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Section: Discussionmentioning

confidence: 99%

Application of cerium chloride to improve the acid resistance of dentine

Wegehaupt

Sener

Attin

et al. 2010

Archives of Oral Biology

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“…The efficacy of waiting periods to increase abrasion resistance is still questioned, since data are conflicting. Some studies show that abrasion resistance is significantly increased by storage in artificial saliva [Attin et al, 2000] or by intra-oral exposure [Jaeggi and Lussi, 1999;Attin et al, 2001bAttin et al, , 2004Rios et al, 2006b], while others found no effect [Attin et al, 2001a;Hara et al, 2003;Ganss et al, 2007b;SalesPeres et al, 2007;Kato et al, 2009]. However, a time delay before brushing might better represent conditions in daily life, as it seems rather unlikely that people brush their teeth immediately after each erosive challenge.…”

Section: Interim Storage (In Vitro) and Intra-oral Exposure (In Situ)mentioning

confidence: 99%

Design of Erosion/Abrasion Studies – Insights and Rational Concepts

Wiegand

Attin²

2011

Caries Res

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In vitro and in situ studies modelling the wear of dental hard tissues due to erosion and abrasion are characterised by a high variation in study designs and experimental parameters. Based on a summary of the existing protocols, the present review aimed to describe and discuss the parameters which must be carefully considered in erosion-abrasion research, especially when it is intended to simulate clinical conditions. Experimental characteristics and parameters were retrieved from a total of 42 in vitro and 20 in situ studies. The key experimental characteristics included parameters of erosion (duration and pH) and abrasion (duration, kinds of toothbrush and toothpaste, brushing force, and time point) as well as cofactors (e.g. dental hard tissue). The majority of studies used models with alternating erosion/abrasion treatments intended to simulate clinical conditions, while other studies exaggerated clinical conditions intentionally, often using only a single erosion/abrasion treatment. Both in vitro and in situ models shared a high level of standardisation, but several studies showed a trend to severe erosion (e.g. >5 min/cycle) or extensive brushing (e.g. >100 brushing strokes/cycle) at a high frequency and repetition rate. Thus, studies often tend to produce a higher amount of wear than in the clinical situation, especially as modifying biological factors (e.g. the dilution of the erosive solution by saliva and the protective effect of the pellicle) cannot be simulated adequately. With respect to the existing models, it seems advisable to diminish duration and frequency of erosion and abrasion to more realistic clinical conditions when the everyday situation is to be simulated. Experimental parameters must be chosen with care to ensure that the problem is investigated in an appropriate mode at standardised conditions and with adequate measuring systems to allow prediction of clinical outcomes. strokes/cycle) at a high frequency and repetition rate. Thus, studies often tend to produce a higher amount of wear than in the clinical situation, especially as modifying biological factors (e.g. the dilution of the erosive solution by saliva and the protective effect of the pellicle) cannot be simulated adequately. With respect to the existing models, it seems advisable to diminish duration and frequency of erosion and abrasion to more realistic clinical conditions when the everyday situation is to be simulated. Experimental parameters must be chosen with care to ensure that the problem is investigated in an appropriate mode at standardised conditions and with adequate measuring systems to allow prediction of clinical outcomes. Copyright © 2011 S. Karger AG, Basel In recent years, extensive research has been undertaken in order to gain a better insight into and understanding of the processes involved in the development of erosive tooth wear. Erosion, abrasion and attrition rarely act alone but interact with each other, and abrasion of erosively altered dental hard tissues is considered as the most import...

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“…[27][28][29] Reasons for using bovine dentine as substrate for these studies might be that it is easier to obtain a sufficient number of sound bovine teeth instead of human teeth. 30 Furthermore, the bovine teeth, in contrast with human teeth, have no caries or fluoride application history that might influence the erosive tooth wear or the interaction of dentine with fluorides or other applied chemical substances.…”

Section: Discussionmentioning

confidence: 99%

Anti-erosive potential of amine fluoride, cerium chloride and laser irradiation application on dentine

Wegehaupt

Sener

Attin

et al. 2011

Archives of Oral Biology

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METHODS: Ninety-six dentine samples were prepared from human premolars and randomly assigned to eight groups (G1-G8). Samples were treated for 30s with the following solutions: placebo (G1/G2), amine fluoride (Elmex fluid; G3/G4), cerium chloride (G5/G6) and combined fluoride/cerium chloride application (G7/G8). Samples of groups G2, G4, G6 and G8 were additionally irradiated with a carbon dioxide laser through the solutions for 30s. Acid resistance was assessed in a six-time 5-min consecutive lactic acid (pH 3.0) erosion model and calcium release was determined by atomic absorption spectroscopy (AAS). Furthermore, six additional samples per group were prepared and subjected to EDS-analysis. RESULTS: In the non-irradiated groups, specimens of G1 (placebo) showed the highest calcium release when compared to the other treatments (G3, G5 and G7). The highest acid resistance was observed for G7. In G3, calcium release was lower than in G5, but higher than in G7. In general (except for the placebo groups), calcium release in the laser-irradiated groups was higher compared with the respective non-irradiated groups. EDS showed a replacement of calcium by cerium and of phosphor by fluoride. CONCLUSION: The highest anti-erosive potential was found after combined cerium chloride and amine fluoride application. Laser irradiation had not adjunctive effect. Abstract:Methods: Ninety-six dentine samples were prepared from human premolars and randomly assigned to eight groups (G1 to G8). Samples were treated for 30 s with the following solutions: placebo (G1/G2), amine fluoride (Elmex fluid; G3/G4), cerium chloride (G5/G6) and combined fluoride/cerium chloride application (G7/G8). Samples of groups G2, G4, G6 and G8 were additionally irradiated with a carbon dioxide laser through the solutions for 30 s. Acid resistance was assessed in a six-time 5-minutes consecutive lactic acid (pH 3.0) erosion model and calcium release was determined by atomic absorption spectroscopy (AAS). Furthermore, six additional samples per group were prepared and subjected to EDS-analysis.Results: In the non-irradiated groups, specimens of G1 (placebo) showed the highest calcium release when compared to the other treatments (G3, G5 and G7). The highest acid resistance was observed for G7. In G3, calcium release was lower than in G5, but higher than in G7. In general (except for the placebo groups), calcium release in the laser-irradiated groups was higher compared with the respective non-irradiated groups.EDS showed a replacement of calcium by cerium and of phosphor by fluoride.Conclusion: The highest anti-erosive potential was found after combined cerium chloride and amine fluoride application. Laser irradiation had not adjunctive effect. Introduction:

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In vitro evaluation of different remineralization periods in improving the resistance of previously eroded bovine dentine against tooth-brushing abrasion

Cited by 41 publications

References 15 publications

Application of cerium chloride to improve the acid resistance of dentine

Application of cerium chloride to improve the acid resistance of dentine

Design of Erosion/Abrasion Studies – Insights and Rational Concepts

Anti-erosive potential of amine fluoride, cerium chloride and laser irradiation application on dentine

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