In-Vitro Evaluation of Cytotoxicity and Antiinflammatory Potential of Hypnea Musciform (Rhodophyta) Collected from the Coastal Area of Karachi, Pakistan

Abstract

Background: The extensive littoral zones of Karachi, Pakistan, are home to a diverse marine phytoplankton population, including the underutilized Hypnea musciformis. This red algae is an unexplored potential source of bioactive compounds for pharmacological use. Objective: This study aimed to evaluate the cytotoxicity and anti-inflammatory potential of the aqueous extract of Hypnea musciformis collected from the coastal areas of Karachi. Methods: Hypnea musciformis was collected from November 2019 to February 2021, with environmental parameters such as seawater temperature, pH, and salinity recorded. The collected samples were dried, powdered, and extracted in water. The cytotoxicity was assessed using a brine shrimp lethality test across a range of concentrations (1, 10, 100, and 1000 µg/ml), determining the LC50 value. Anti-inflammatory activity was evaluated via a membrane stabilization method on human red blood cells (HRBC), comparing the effects with the standard drug Diclofenac at concentrations ranging from 100 to 200 µg/ml. Results: The brine shrimp lethality assay showed a dose-dependent increase in mortality with the highest concentration exhibiting 83% mortality, yielding an LC50 value of 550 µg/ml. The anti-inflammatory assay demonstrated that the aqueous extract of Hypnea musciformis provided a concentration-dependent protection with a maximum protection percentage of 78.64% at 200 µg/ml, while the control substance, Diclofenac, displayed higher efficacy. Conclusion: The aqueous extract of Hypnea musciformis possesses significant cytotoxic and anti-inflammatory activities, suggesting its potential as a source of natural compounds for therapeutic purposes. However, further studies are required to elucidate the mechanisms of action and to confirm the safety and effectiveness of the extract in clinical applications.