In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay

Boeckel, Daniel Gonçalves; Shinkai, Rosemary Sadami Arai; Grossi, Márcio Lima; Teixeira, Eduardo Rolim

doi:10.1016/j.oooo.2012.07.486

Cited by 27 publications

(21 citation statements)

References 30 publications

(46 reference statements)

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“…HA is known as a biocompatible polymer and studies have shown that it is safe for concentrations up to 1-2% (W/V) [17,18]. Comparing the results obtained for the HA and HASSNH 2 polymers, no statistical differences were observed in all four tested situations.…”

Section: Polymer Modification Did Not Affect Cytotoxicitymentioning

confidence: 82%

Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal gene vector

Oliveira

Marcelo

Costa

et al. 2016

Materials Science and Engineering: C

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Purpose: A successful gene therapy approach can prevent or treat congenital and acquired diseases. However, there is still no ideal non-viral vector for gene delivery in a safe and timely manner. In this report the anionic polymer hyaluronic acid (HA) was investigated as a potential vector for gene therapy. Due to its intrinsic characteristics it constitutes an excellent candidate to deliver therapeutic genes, pending the modification of its surface charge. Methods: To modify its charge, HA was modified with cystamine. Several formulations were prepared using modified HA combined with sodium sulfate, sodium triphosphate, K-carrageenan and chitosan. Vectors were characterized with respect to size, charge, DNA load and its protection, and effect on cell viability. The better performing formulations were further evaluated in vitro for their transfection efficiency in HEK293T and ARPE-19 cells. Results: Cell viability assays showed low cytotoxicity for both polymers. Gene transfer efficiency depended on cell line and formulation, but no increased transfection efficiency was observed with the modified polymer. Conclusions: HA has great potential as a gene therapy vector, but further optimization, including incorporation of a higher percentage of positive groups in HA, is needed before its use as a gene delivery vector.

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Section: Polymer Modification Did Not Affect Cytotoxicitymentioning

confidence: 82%

Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal gene vector

Oliveira

Marcelo

Costa

et al. 2016

Materials Science and Engineering: C

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“…Other researchers reported that the proliferation of cells cultured with the addition of high‐MW HA was not affected when compared with that of cells cultured without HA . However, other studies showed that the cell proliferation was inhibited with the presence of high‐MW HA . In one study, the researchers coated the culture plates with HA of varying MW under serum‐free conditions before cell culture, instead of direct addition of HA.…”

Section: Effect Of Ha On Cell Proliferation and Differentiation In Vitromentioning

confidence: 99%

“…Besides these receptors, some other factors have to be considered regarding the effect of MW and concentration of HA on cell proliferation and differentiation. On one hand, the delivering method of HA might play an important role . Different delivering methods of HA can lead to different length of HA–cell interaction time.…”

Section: Mechanism Of Ha On Cell Proliferation and Osteogenic Differementioning

confidence: 99%

“…29,35,38 However, other studies showed that the cell proliferation was inhibited with the presence of high-MW HA. 39,40 In one study, the researchers coated the culture plates with HA of varying MW under serum-free conditions before cell culture, instead of direct addition of HA. The results indicated that HA-coated plates were rougher and more hydrophilic with higher MW HA, leading to the decrease of cell adhesion, cell-matrix interaction, and proliferation of rabbit corneal keratocytes.…”

Section: Effect Of Ha On Cell Proliferation and Differentiation In Vitromentioning

confidence: 99%

“…On one hand, the delivering method of HA might play an important role. 29,30,[36][37][38][39][40] Different delivering methods of HA can lead to different length of HA-cell interaction time. For example, the cells cultured in plates coated by HA have relatively longer HA-cell interaction time than that of the cells cultured with HA added only once during the first plating of cells in the initial medium.…”

Section: Mechanism Of Ha On Cell Proliferation and Osteogenic Differementioning

confidence: 99%

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Effect of hyaluronic acid in bone formation and its applications in dentistry

Zhao

Wang

Qin

et al. 2016

J Biomedical Materials Res

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Hyaluronic acid (HA), the simplest glycosaminoglycan, participates in several important biological procedures, including mediation of cellular signaling, regulation of cell adhesion and proliferation, and manipulation of cell differentiation. The effect of HA on cell proliferation and differentiation depends on its molecular weight (MW) and concentration. Moreover, the properties of high viscosity, elasticity, highly negative charge, biocompatibility, biodegradability, and nonimmunogenicity make HA attractive in tissue engineering and disease treatment. This review comprises an overview of the effect of HA on cell proliferation and differentiation in vitro, the role of HA in bone regeneration in vivo, and the clinical applications of HA in dentistry, focusing on the mechanism underlining the effect of MW and concentration of HA on cell proliferation and osteogenic differentiation. It is expected that practical progress of HA both in laboratory-based experiments and clinical applications will be achieved in the next few years. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1560-1569, 2016.

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Laser‐Assisted Fabrication of Injectable Nanofibrous Cell Carriers

Nakielski

Rinoldi

Pruchniewski

et al. 2021

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The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell‐based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser‐assisted micro‐scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell‐populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in‐depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications.

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In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay

Abstract: Results suggested a decrease in cell viability in the presence of HA.

Cited by 27 publications

References 30 publications

Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal gene vector

Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal gene vector

Effect of hyaluronic acid in bone formation and its applications in dentistry

Laser‐Assisted Fabrication of Injectable Nanofibrous Cell Carriers

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