In vitro evaluation of contact-active antibacterial efficacy of Ti-Al-V alloys coated with the antimicrobial agent PHMB

Hornschuh, Melanie; Zwicker, Paula; Schmidt, Thomas; Krämer, Axel; Müller, Gerald

doi:10.1016/j.actbio.2020.02.016

Cited by 18 publications

(15 citation statements)

References 44 publications

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“…These results are in accordance with the literature. In addition, the antimicrobial surface is desirable properties of biomaterials for biomedical application [37][38][39][40][41][42][43][44][45][46][47] .…”

Section: Resultsmentioning

confidence: 99%

Coated Surface on Ti-30Ta Alloy for Biomedical Application: Mechanical and in-vitro Characterization

et al. 2020

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Several studies have been carried out to develop new materials for biomedical applications. Material surfaces that present biomimetic morphology like nanotubes or nanofibers that provides nanoscale architectures have been shown to alter cell/biomaterial interactions. The coated surface biomaterial with biocompatible polymers and nanotubes of TiO 2 is an alternative to improve osseointegration. The anodization process was performed to obtain nanotubes of TiO 2 covering the Ti-30Ta alloy surface and the electrospinning process has been used for producing polymer fibers. Characterization techniques such as scanning electron microscopy (SEM-FEG), X-ray diffraction analysis (X-rays), thermogravimetric analysis (TGA), Differential Scanning Calorimetry (DSC) and contact angle were used for samples analyses. Adult human adipose-derived stem cells (ADSCs) were used to investigate the cellular response and S. aureus antimicrobial activity on these coated surfaces. The results indicated that both surface modification treatment showed a favorable micro-environment for cells growth and proliferation such as adhesion, viability and morphology which is a desire property for an implant. In addition, the antimicrobial activity study presented both materials with similar growth of S. aureus. So, it can conclude nanotubes and nanofibers can be used at biomedical field and both present similar cell evaluation and antimicrobial activity results.

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Section: Resultsmentioning

confidence: 99%

Coated Surface on Ti-30Ta Alloy for Biomedical Application: Mechanical and in-vitro Characterization

et al. 2020

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“…Thus, in contrast to unmodified surfaces, oxidation with H 2 O 2 resulted in a 10 times higher PHMB coverage of 1.36 ± 0.12 µg per test specimen [8]. Hornschuh et al demonstrated a binding capacity of H 2 O 2 oxidized surfaces up to 3-4 µg per test specimen [12]. The increased adsorption was ascribed to a removal of impurities on the titanium surface and to the generation of more possible binding sites in the form of OH − -groups, as revealed by Tengvall et al [13].…”

Section: Introductionmentioning

confidence: 99%

“…With this method, the amount of PHMB adsorption is adjustable by incubation time in the coating solution. After 24 h, a PHMB adsorption of up to 20.9 ± 0.6 µg per test specimen was detected, and after 48 h, equilibrium was reached [9,12]. While the increased hydroxylation enhances PHMB adsorption, it also attracts more volatile hydrocarbons, leading to a fast contamination of the surface.…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies by Müller et al and Hornschuh et al, the surface of Ti6Al4V alloy material was modified by oxidation with H 2 O 2 and pre-treatment with NaOH, respectively, following adsorption of PHMB to the surface by incubation in aqueous solution [8,9,12]. Müller et al stated that a hydroxylated surface is necessary for PHMB absorption, as a simple cleaning of the surface without the generation of extra binding sites did not result in stronger PHMB binding [8].…”

Section: Introductionmentioning

confidence: 99%

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Improved Adsorption of the Antimicrobial Agent Poly (Hexamethylene) Biguanide on Ti-Al-V Alloys by NaOH Treatment and Impact of Mass Coverage and Contamination on Cytocompatibility

et al. 2021

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Unlike the native surface of the implant material (Ti6Al4V), oxidation with H2O2 leads to increased binding of the effective antimicrobial agent poly(hexamethylene) biguanide [PHMB]. However, treating with NaOH instead results in an even higher PHMB mass coverage. After oxidation with H2O2, strong differences in the PHMB adsorption capability between polished and corundum-blasted surfaces appear, indicating a roughness dependence. After NaOH treatment, no such effect was observed. The wetting properties of specimens treated with either H2O2 or NaOH prior to PHMB exposure clearly varied. To unravel the nature of this interaction, widespread in silico and in vitro experiments were performed. Methods: By X-ray photoelectron spectroscopy, scanning electron microscopy, water contact angle measurements and MD simulations, we characterized the interplay between the polycationic antimicrobial agent and the implant surface. A theoretical model for PHMB micelles is tested for its wetting properties and compared to carbon contaminated TiO2. In addition, quantitation of anionic functional group equivalents, the binding properties of PHMB with blocked amino end-group, and the ability to bind chlorhexidine digluconate (CHG) were investigated. Ultimately, the capability of osteoblasts to build calcium apatite, and the activity of alkaline phosphatase on PHMB coated specimens, were determined. Results: Simulated water contact angles on carbon contaminated TiO2 surfaces and PHMB micelle models reveal little influence of PHMB on the wetting properties and point out the major influence of remaining and recovering contamination from ambient air. Testing PHMB adsorption beyond the critical micelle concentration and subsequent staining reveals an island-like pattern with H2O2 as compared to an evenly modified surface with NaOH. Both CHG and PHMB, with blocked amino end groups, were adsorbed on the treated surfaces, thus negating the significant influence of PHMB’s terminal groups. The ability of osteoblasts to produce calcium apatite and alkaline phosphatase is not negatively impaired for PHMB mass coverages up to 8g/specimen. Conclusion: Differences in PHMB adsorption are triggered by the number of anionic groups and carbon contaminants, both of which depend on the specimen pre-treatment. With more PHMB covering, the implant surface is protected against the capture of new contamination from the ambient air, thus building a robust antimicrobial and biocompatible surface coating.

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“…Biguanide has a cationic functional group and numerous studies have focused on its bactericidal effect. [13][14][15][16][17] These groups bind to the phospholipid head groups of the anion-charged plasma membrane through ionic interactions. Binding of biguanide fungicides to phospholipids on the outer leaflets of the plasma membrane is believed to impair membrane fluidity.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal effect of cationic hydrogels prepared from hydrophilic polymers

Shibata

Kurokawa

Aizawa

et al. 2020

J of Applied Polymer Sci

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This study investigated whether hydrogels comprising hydrophilic cationic polymers have similar bactericidal effects. Bacteria were seeded on hydrogels and agar and their viability was assessed with time. Cationic hydrogels displayed bactericidal effects upon long‐term bacterial contact. Furthermore, we assessed the areal density of cationic monomer unit of the cationic hydrogels, water content, and the initial elastic modulus. We examined correlations between each factor and bacterial death ratios; consequently, the bacterial death ratios were strongly correlated with the areal density of cationic hydrogel monomers. Elastic energy (Wel) generated at the cytomembrane ion‐binding region and the cationic hydrogel and the cytomembrane interfacial energy (Wf) were estimated; consequently, Wel exceeded Wf at higher contact areas. The cationic hydrogel may extract cytomembranes with a reasonable adsorption area. Therefore, cationic hydrogels may be used as probes for ultrasonic echo to sterilize medical equipment.

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In vitro evaluation of contact-active antibacterial efficacy of Ti-Al-V alloys coated with the antimicrobial agent PHMB

Cited by 18 publications

References 44 publications

Coated Surface on Ti-30Ta Alloy for Biomedical Application: Mechanical and in-vitro Characterization

Coated Surface on Ti-30Ta Alloy for Biomedical Application: Mechanical and in-vitro Characterization

Improved Adsorption of the Antimicrobial Agent Poly (Hexamethylene) Biguanide on Ti-Al-V Alloys by NaOH Treatment and Impact of Mass Coverage and Contamination on Cytocompatibility

Bactericidal effect of cationic hydrogels prepared from hydrophilic polymers

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