In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp

Abstract

Interest in periocular (eyelid and eyelashes margins) hygiene has attracted attention recently and a growing number of commercials eye cleanser and shampoos have been marketed. In the present study, a particular eye cleanser foam, Belcils® has been tested against trophozoites and cysts on the facultative pathogen Acanthamoeba . Viability was tested by the alamarBlue™ method and the foam was tested for the induction of programmed cell death in order to explore its mode of action. We found that a 1% solution of the foam eliminated both trophozoite and cyst stage of Acanthamoeba spp. After 90 min of incubation, Belcils® induced, DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba . We conclude that the foam destroys the cells by the induction of an apoptosis-like process. The current eye cleanser could be used as part of AK therapy protocol and as prevention from AK infections for contact lens users and post-ocular trauma patients.

Antiamoeboid activity of squamins C–F, cyclooctapeptides from Annona globifora

“…The experiment was carried out by following the manufacturer's recommendations, and 10 5 cells/mL well were incubated for 24 h with the previously calculated IC 90 of squamins C–F ( 1 – 4 ). As previously reported ( Sifaoui et al, 2020 ), the double-staining pattern allows the identification of three groups in a cellular population: live cells will show only a low level of blue fluorescence, cells undergoing PCD will show a higher level of blue fluorescence (as chromatin condenses), and dead cells will show low-blue and high-red fluorescence (as the Propidium Iodide stain (Life Technologies, Madrid, Spain) enters the nucleus).…”

In vitro amoebicidal effects of arabinogalactan-based ophthalmic solution

“…Moreover, other corneal disorders such as the dry eye syndrome, is other disease derived from the use of contact lenses. Lately, diverse research studies have reported the efficacy of commercialized eye drops and ophthalmic solutions used for these other epithelial disorders, against the two life cycle stages of Acanthamoeba strains ( Sifaoui et al, 2017 , 2018 , 2020a , b ; Reyes-Batlle et al, 2019 ). Several commercialized ophthalmic solutions present preservatives such as Propylene Glycol, Benzalkonium chloride (BAK) or its derivatives such as POLYQUAD®, which have demonstrated antimicrobial ( Kinnunen et al, 1991 ; Nalawade et al, 2015 ) or anti- Acanthamoeba activity ( Sunada et al, 2014 ).…”