In vitro Evaluation of Botanical Extract, Bioagents and Fungicides against Purple Blotch Diseases of Bunch Onion in Bangladesh

Rahman, Syed Ajijur; Sikder, Md. Maniruzzaman; Nusrat, Shohana; Khair, Abul

doi:10.13189/azb.2015.030403

Cited by 13 publications

(11 citation statements)

References 8 publications

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“…While, the minimum mycelial inhibition (66.66%) was noticed in T. koningii (Table 2 and Plate 1). Similar findings were observed by (Kumari et al, 2006) [6] (Pramodkumar, 2007) [10] , (Kareem, 2008) [5] , (Mishra and Gupta; Wanggikar, 2012) [8] and Rahman et al (2015) [11] who reported that T. viride as effective under in vitro in inhibiting the maximum mycelial growth of the fungus. Among the bacterial bioagents, Pseudomonas fluorescens-4 i.e.…”

Section: Resultssupporting

confidence: 82%

Efficacy of bio control agents under in vitro against Alternaria porri (Ellis) Cifferi. Causing purple blotch in onion

Hariprasad¹,

Palakshappa²,

Dinesh³

et al. 2021

Pharma Innovation

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Section: Resultssupporting

confidence: 82%

Efficacy of bio control agents under in vitro against Alternaria porri (Ellis) Cifferi. Causing purple blotch in onion

Hariprasad¹,

Palakshappa²,

Dinesh³

et al. 2021

Pharma Innovation

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“…The bio-control agents-Bacillus subtilis, Pseudomonas fluorescens, Trichoderma harzianum and two isolates of Trichoderma reesei were evaluated against mycelial growth of the fungus using dual culture technique (Bhadra et al, 2014). The effect of fungicides, namely Tilt 250 EC (Propiconazole) and Amistar Top 325 SC (Azoxystrobin + Difenoconazole) on the radial growth of the fungus was determined on PDA medium using the food poison technique (Rahman et al, 2015). A requisite quantity of fungicides was added to the medium with a concentration of 250 ppm, 500 ppm, and 750 ppm.…”

Section: Efficacy Of Bio-control Agents and Fungicides Against F Solanimentioning

confidence: 99%

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Mallik

Sikder

Hossain

et al. 2022

Int. J. Agril. Res. Innov. & Tech.

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Fruit rot disease of sweet pepper is one of the main fungal diseases causing huge economic losses to the grower. An experiment was conducted to find out the fungal pathogen associated with fruit rot disease of sweet pepper, obtained from experimental fields of Jahangirnagar University, Bangladesh. Fruit rot disease-causing fungus was isolated from infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well as molecular characterization based on internal transcribe spacer (ITS) region of the fungus. ITS sequence of our studied fungus MH368146.1 was genetically 99-100% similar to sequences of Fusarium solani in NCBI database. Typical fruit rot symptoms were reproduced by artificial inoculations of the isolated fungus. The mycelial growth of this fungus was evaluated on ten different solid culture media i.e., Potato Dextrose Agar, Yeast Extract Agar, Honey Peptone Agar, Hansen’s Medium, Sabouraud’s Glucose Agar, Kauffman’s Agar, Potato Sucrose Agar, Richard’s Agar and Carrot Agar. Fungus grew well on all tested solid culture media. Several bio-control agents and two commercial fungicides were evaluated against isolated fungus under in vitro condition, in which the highest percent inhibition of radial growth of the fungus was determined as 64.75% due to Trichoderma reesei isolate 2, and 60.63% by Tilt 250 EC (500 ppm) at 7 days post-incubation. Therefore, T. reesei was found as the most suitable to control the growth of F. solani under laboratory conditions. However, further pot and field trials needed to be confirmed the bio-control potential of it. Int. J. Agril. Res. Innov. Tech. 11(2): 108-116, Dec 2021

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“…The ethanolic plant extracts were prepared from leaves of Tagetes erecta L., Azardirachta indica L., and Lowsonia innermis L. and each plant extract of different concentrations (10%, 20% and 30%) were mixed with PDA as different treatment combinations (Rahman et al, 2015). Inoculated petri-plates were kept in an incubation chamber at 25±2°C.…”

Section: Efficacy Of Bio-control Agents Plant Extracts and Fungicides Against Curvularia Lunatamentioning

confidence: 99%

Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures

Billah

Sikder

Mallik

et al. 2022

Int. J. Agril. Res. Innov. & Tech.

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Present studies were conducted to isolate and identify the seed-borne pathogenic fungus from the selected tomato variety through morphological and molecular techniques based on the sequencing of internal transcribed spacer (ITS) region of 18S rDNA. According to the colony and conidial features, the fungus was identified as Curvularia sp. The obtained ITS sequencing showed above 99% similarity with Curvularia lunata in the NCBI database. The sequence of the fungus was deposited in NCBI GenBank under the accession number: ITS, MH382879.1. Besides, the phylogenetic tree further confirmed the taxonomic position of the studied fungus. Growth characteristics of the fungus on nine different fungal culture media were evaluated, in which Honey peptone agar, Carrot agar, Potato sucrose agar, and Kauffman’s agar were found the most suitable. The maximum vegetative growth of the fungus was recorded at 30°C temperature and pH conditions. The bio-control potential of five different antagonists against the studied fungus was assessed, in which Trichoderma harzianum showed the better performance to restrict mycelial growth. Three ethanolic plant extracts were also evaluated, in which Lowsonia inermis L. exhibited above 60% mycelial growth inhibition of the fungus. Among three tested fungicides, Tilt 250 EC was found as an excellent fungicide to inhibit mycelial growth of C. lunata under in vitro conditions. Int. J. Agril. Res. Innov. Tech. 11(2): 124-132, Dec 2021

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In vitro Evaluation of Botanical Extract, Bioagents and Fungicides against Purple Blotch Diseases of Bunch Onion in Bangladesh

Cited by 13 publications

References 8 publications

Efficacy of bio control agents under in vitro against Alternaria porri (Ellis) Cifferi. Causing purple blotch in onion

Efficacy of bio control agents under in vitro against Alternaria porri (Ellis) Cifferi. Causing purple blotch in onion

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures

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