In vitro evaluation of bioactive properties of banana sap

Abstract

Banana sap is currently designated as a waste subsequent to utilization of pseudo stem in pulp and paper industry as well as other applications which is contributing to the environmental pollution. In the present study, banana sap and its crude extracts were evaluated for antimicrobial, antioxidant and anticancer properties. The role of oxidized and un-oxidized banana sap for its antimicrobial potential against a microbial test panel comprising gram positive as well as gram negative bacteria and Candida albicans using in vitro micro broth dilution assay. The un-oxidized banana sap exhibited a significantly higher antibacterial potential as evident by a lower minimal inhibitory concentration (MIC) ranging between 15.625 to 62.5 mg/mL. In vitro radical scavenging activity of dichloromethane (DCM) extract of banana sap by DPPH method exhibited 54.62 ± 1.09 (µg/mL) IC 50 value at the concentration of 1 mg/mL. Dichloromethane extract of banana sap showed maximum cytotoxic effect with human breast cancer (MCF-7) cell proliferation at the concentration of 100 µg/mL which was 78.37 ± 0.05% and the cytotoxic effect significantly increased with increasing concentration of banana sap extract. Furthermore, LCMS studies revealed the presence of bioactive compounds in dichloromethane extract of banana sap, such as rescinnamine derivative, dihydrorescinnamine and epimedin A. The present study suggested that banana sap is a promising source of bioactive compounds with relevant antimicrobial, antioxidant and anticancer properties.

Banana and its by‐products: A comprehensive review on its nutritional composition and pharmacological benefits

“…The banana pseudo stem extract containing flavanol and flavones is cytotoxic against the cancer cells MCF‐7 (breast cancer) (Gupta et al, 2022). Based on the presence of glycosides, alkaloids, phenols, flavonoids, and saponins in banana pseudo stem extract, it showed higher cytotoxicity against HCT‐116 (86.53) and HepG‐2 cell lines (79.06) compared with other banana pseudo stem extracts (Abdel Ghany et al, 2019; Ogbonna et al, 2016).…”

Enhancing corrosion resistance of mild steel in hydrochloric acid with Chiquita banana sap extract

“…Besides, tannins present in banana sap cause the sap to darken when exposed to air, demonstrating appearance of carbohydrate and suggesting its potential for long-term rust prevention with environmentally sustainable properties. 31,32 The main objective of this investigation is to study the electrochemical response of natural plant waste to mitigate the corrosive effects of hydrochloric acid on mild steel. To achieve this, we used a banana sap-water extract (BSWE) to limit the deterioration of mild steel, which is known to be prone to corrosion in acidic environments.…”

“…Besides, tannins present in banana sap cause the sap to darken when exposed to air, demonstrating appearance of carbohydrate and suggesting its potential for long-term rust prevention with environmentally sustainable properties. 31,32…”