In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells

Wu, Di; Zhang, Miao; Xu, Jiao; Song, Erwei; Lv, Yinjun; Tang, Shu; Zhang, Xiaohui; Kemper, Nicole; Hartung, J.; Bao, Endong

doi:10.1007/s12192-016-0666-8

Cited by 31 publications

(19 citation statements)

References 49 publications

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“…According to a series of markers for injury evaluation, aspirininduced HS resistance, which has been reported before (Wu et al 2015(Wu et al , 2016, was also observed in this study. Similar to the former studies, aspirin pretreatment inhibited the increased levels of the myocardial cell damage-related enzymes CK-MB and LDH and the pathological changes.…”

Section: Discussionsupporting

confidence: 76%

“…Cell culture and stress model Primary cultures of chicken myocardial cells (provided by Shanghai Fu Meng Biological Technology Ltd, Shanghai, China) were cultured as we described before (Wu et al 2016). Cells were cultured (37°C and 5 % CO 2 ) on the Petri dishes for 72 h until the fusion rate was higher than 90 %.…”

Section: Methodsmentioning

confidence: 99%

“…The damages caused by heat stress to breeding industries have been explained in our previous works, especially the considerable economic loss on poultry breeding (Wu et al 2015(Wu et al , 2016. Previous research also has revealed that acute HS leads to cardiac dysfunction and other forms of myocardial cell damage (Tang et al 2013), and these effects are responsible for sudden infant death in animals and even in humans after HS exposure (Gathiram et al 1987(Gathiram et al , 1988Rai and Ambwany 1980).…”

Section: Introductionmentioning

confidence: 92%

“…The protective function of aspirin against heat stress has been better presented in our former research (Wu et al 2016). In this study, we focused on the differences between group HS (HS challenge group) and group A (with aspirin pretreatment before HS) in short-term heat stress response.…”

Section: Differences Caused By Involving Aspirin Into Heat Stress Resmentioning

confidence: 99%

“…Based on the above factors, in our recent studies, we inferred that the heat stress resistance function of aspirin in the myocardial cells of chickens is related to the expression of some HSPs, particularly HspB1 (also called Hsp27) (Wu et al 2015(Wu et al , 2016. However, more evidence is required to support the association between aspirin-induced heat stress resistance and the expression of HSPs in myocardial cells.…”

Section: Introductionmentioning

confidence: 98%

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Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro

Zhang

et al. 2016

Cell Stress and Chaperones

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Our recent studies have displayed the protective functions of aspirin against heat stress (HS) in chicken myocardial cells, and it may be associated with heat shock proteins (HSPs). In this study, we further investigated the potential role of HSPs in the aspirin-induced heat stress resistance. Four of the most important HSPs including HspB1 (Hsp27), Hsp60, Hsp70, and Hsp90 were induced by aspirin pretreatment and were suppressed by BAPTA-AM. When HSPs were induced by aspirin, much slighter HS injury was detected. But more serious damages were observed when HSPs were suppressed by BAPTA-AM than those cells exposed to HS without BAPTA-AM, even the myocardial cells have been treated with aspirin in prior. Comparing to other HSPs, HspB1 presented the largest increase after aspirin treatments, 86-fold higher than the baseline (the level before HS). These findings suggested that multiple HSPs participated in aspirin's anti-heat stress function but HspB1 may contribute the most. Interestingly, during the experiments, we also found that apoptosis rate as well as the oxidative stress indicators (T-SOD and MDA) was not consistently responding to heat stress injury as expected. By selecting from a series of candidates, myocardial cell damage-related enzymes (CK-MB and LDH), cytopathological tests, and necrosis rate (measured by flow cytometry assays) are believed to be reliable indicators to evaluate heat stress injury in chicken's myocardial cells and they will be used in our further investigations.

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Section: Discussionsupporting

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Differences Caused By Involving Aspirin Into Heat Stress Resmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro

Zhang

et al. 2016

Cell Stress and Chaperones

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HSP47 in human diseases: Navigating pathophysiology, diagnosis and therapy

Khan,

Däinghaus

2024

Clinical & Translational Med

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Heat shock protein 47 (HSP47) is a chaperone protein responsible for regulating collagen maturation and transport, directly impacting collagen synthesis levels. Aberrant HSP47 expression or malfunction has been associated with collagen‐related disorders, most notably fibrosis. Recent reports have uncovered new functions of HSP47 in various cellular processes. Hsp47 dysregulation in these alternative roles has been linked to various diseases, such as cancer, autoimmune and neurodegenerative disorders, thereby highlighting its potential as both a diagnostic biomarker and a therapeutic target. In this review, we discuss the pathophysiological roles of HSP47 in human diseases, its potential as a diagnostic tool, clinical screening techniques and its role as a target for therapeutic interventions.

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TMT‐labeled quantitative proteomic analysis to identify proteins associated with the stability of peanut milk

et al. 2021

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BACKGROUND: Peanut milk benefits human health mainly due to its high protein content and suitable amino acid composition. To reveal the molecular mechanism affecting the quality of peanut milk, tandem mass tag (TMT)-labeled proteomic analysis was applied to identify the proteome variation between two peanut cultivars that produced peanut milk with the best and worst stability.RESULTS: A total of 478 differentially abundant proteins (fold change >1.2 or <0.83, P < 0.05) were identified. Most of these proteins were located in the cytoplasm and chloroplasts. Correlation analysis showed that RNA recognition motif (RRM) domain-containing protein (17.1 kDa) had a negative relationship with the sedimentation rate of peanut milk and that 22.0 kDa class IV heat shock protein was negatively correlated with the creaming index (P < 0.05). Bioinformatic analysis showed that the molecular function of RRM domain-containing protein (17.1 kDa) was associated with RNA binding and nucleotide binding, and 22.0 kDa class IV heat shock protein was involved in the pathway of protein processing in the endoplasmic reticulum.CONCLUSION: Overall, the differentially abundant proteins in the biological metabolic pathway might offer some potential markers to guide future peanut breeding, especially for the production of peanut milk.

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In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells

Cited by 31 publications

References 49 publications

Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro

Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro

HSP47 in human diseases: Navigating pathophysiology, diagnosis and therapy

TMT‐labeled quantitative proteomic analysis to identify proteins associated with the stability of peanut milk

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