In vitro evaluation of antioxidative activities of the extracts of petals of Paeonia lactiflora and Calendula officinalis incorporated in the new forms of biobased carriers

Abstract

In this study, the petals collected from peony hybrid Paeonia lactiflora "Bowl of Beauty" and Calendula officinalis L. were extracted using an ethanol-water mixture assisted with microwave and ultrasonic treatment. The isolation of Calendula officinalis L. essential oil was done by hydrodistillation as well. The total phenolic and flavonoids content in the extracts and oil were determined and their antioxidant activity was evaluated. The highest total phenolic content was found for the extracts of hybrid P. lactiflora and C. officinalis L. obtained by ultrasound extraction (83.16 and 114.47 mg GA/g, respectively), while the flavonoid content obtained by microwave-assisted extraction was relatively high (123.48 and 65.29 mg QE/g, respectively). The highest antioxidant activity was obtained in DPPH• and ABTS•+ assay for the microwave-assisted extraction of hybrid P. lactiflora (79% and 83%) and ultrasound-assisted extraction of C. officinalis L. (45% and 49%), respectively. To improve antioxidant activity of both types of examined analytes (extracts and essential oil), the pectin biopolymer film (as a carrier) was prepared in the process of enzymatically assisted catalysis. Optical microscopy and FTIR spectroscopy were used for the characterization of obtained materials. The films, with essential oil of C. officinalis L. and gallic acid, showed significantly increased percentage inhibition in DPPH• and ABTS•+ test (91% and 95%, respectively) after 10 minutes. The results, also, showed that all formulations of pectin biopolymer film, modified with gallic acid, can be successfully applied as a carrier for both types of ingredients.

Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts

“…The analytical protocol described by Prior et al [67], with modifications [68], served as the foundation for the ABTS •+ scavenging assay. Specifically, 200 µL of the petal extract and 2800 µL of the ABTS •+ radical cation solution were combined and incubated at 25 ± 5 • C in the dark for 30 min.…”

Lactate content, total polyphenols and antioxidant activity of selected commercial yogurts from the Serbian market

“…Literature data shows the common use of these tests alone or in combination for the assessment of the antioxidant activity of various plants (Nowicka et al, 2019;Dinkçi, Aktaş, Akdeniz & Sirbu, 2021;Pavlović et al, 2021;Batinić et al, 2022). Results for antioxidative activity determined by the DPPH test and reducing power for methanolic and ethanolic extracts are presented in Table 2.…”