In-vitro evaluation of antioxidant, anti-elastase, anti-collagenase, anti-hyaluronidase activities of safranal and determination of its sun protection factor in skin photoaging

Madan, Kumud; Nanda, Samik

doi:10.1016/j.bioorg.2017.12.030

Cited by 84 publications

(49 citation statements)

References 35 publications

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“…In particular, it is largely known that the modification of shape and number of keratinocytes and fibroblasts is linked to the up- or down-regulation of intra- and extracellular proteins via multifarious cell signaling pathways. Histopathological analysis revealed the massive deterioration of aged skin characterized by a breakdown of collagen type I and elastic fiber as a direct consequence of the activation of MMPs leading to the development of wrinkle formation [ 34 , 35 , 36 , 37 ]. Actually, despite a growing number of children and adults being increasingly exposed to LED-BL, their effects on skin and the rate of damage are poorly known.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Avola

Graziano

Pannuzzo

et al. 2018

Cells

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The development in digital screen technology has exponentially increased in the last decades, and many of today’s electronic devices use light-emitting diode (LED) technology producing very strong blue light (BL) waves. Long-term exposure at LED-BL seems to have an implication in the dehydration of the epidermis, in the alterations of shape and number of the keratinocytes, and in the aging of the skin. Aquaporins (AQPs) are water membrane channels that permeate both water and glycerol and play an important role in the hydration of epidermis, as well as in proliferation and differentiation of keratinocytes. Thus, we have hypothesized that AQPs could be involved in the aging of the skin exposed to LED-BL. Therefore, we have examined the expression of AQPs in human keratinocytes exposed to LED-BL at dose of 45 J/cm2, used as an in vitro model to produce the general features of photo aging of the skin. The aim was to verify if LED-BL induces changes of the basal levels of AQPs. The keratinocytes exposure to LED-BL produced an increase of reactive oxygen species (ROS), an activation of 8-hydroxy-2’-deoxyguanosine (8-OHdG), an alteration of proliferating cell nuclear antigen (PCNA), and a down-regulation of AQP1, 3 and 9. These findings are preliminary evidences that may be used as starting points for further investigations about the mechanistic involvement of AQP1, 3, and 9 in LED-BL-induced skin aging.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Avola

Graziano

Pannuzzo

et al. 2018

Cells

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“…Many researchers have studied natural candidates that can be used as anti-aging agents in the cosmetics industry. They studied the anti-elastase and anti-hyaluronidase activities because these inhibitors are known to play crucial roles in anti-aging of skin by protecting the degradation of elastin and HA, which are ECM components (Kumud and Sanju 2018). Following the degradation of elastin, the skin lost its elasticity and showed wrinkling and sagging (Liyanaarachchi et al 2018).…”

Section: Resultsmentioning

confidence: 99%

“…ROS induce activation of enzymes that are closely related to the degradation of ECM, such as elastase, collagenase, and hyaluronidase (Wittenauer et al 2015). These enzymes are known to be involved in skin aging (Thring et al 2009;Kumud and Sanju 2018).…”

Section: Introductionmentioning

confidence: 99%

Anti-elastase and anti-hyaluronidase activity of phosvitin isolated from hen egg yolk

Lee

Moon

Hong

et al. 2020

British Poultry Science

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1. Phosvitin, a major phosphoprotein found in egg yolk, has strong antioxidant activity. Activation of elastase, collagenase, and hyaluronidase by reactive oxygen species are related to the degradation of ECM and skin aging. The objective of this study was to determine the anti-elastase and antihyaluronidase activity of phosvitin. 2. Elastase from porcine pancreas and hyaluronidase from bovine testes were used to study the inhibitory activity of phosvitin. To elucidate the mechanism of enzyme inhibition, a Lineweaver-Burk plot was constructed. 3. Phosvitin inhibited elastase and hyaluronidase activity in a dose-dependent manner. The IC 50 value of phosvitin was 31.6 μg/ml and 1,270 μg/ml against elastase and hyaluronidase, respectively. The analysis of elastase and hyaluronidase kinetics indicated that the apparent Michaelis constant (app K m) was increased by phosvitin but the V max value was not affected. 4. In conclusion, phosvitin exhibited competitive inhibitory activity against elastase and hyaluronidase. Thus, phosvitin could be used as a natural anti-aging agent in the cosmetics industry.

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“…Common consequences are wrinkle formation and sagging of skin, since these macromolecules are responsible for providing strength, elasticity, and moisture to the skin. [10,11]. Additionally, hyperpigmentation occurs due to excessive accumulation of melanin.…”

Section: Introductionmentioning

confidence: 99%

“…These pollutants include, among others, polycyclic aromatic hydrocarbons, volatile organic compounds, particulate matter, oxides, and heavy metals. They enhance the production of ROS, reducing the content of antioxidants in the skin and causing oxidative stress [11].…”

Section: Introductionmentioning

confidence: 99%

Efficacy, Stability, and Safety Evaluation of New Polyphenolic Xanthones Towards Identification of Bioactive Compounds to Fight Skin Photoaging

et al. 2020

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Antioxidants have long been used in the cosmetic industry to prevent skin photoaging, which is mediated by oxidative stress, making the search for new antioxidant compounds highly desirable in this field. Naturally occurring xanthones are polyphenolic compounds that can be found in microorganisms, fungi, lichens, and some higher plants. This class of polyphenols has a privileged scaffold that grants them several biological activities. We have previously identified simple oxygenated xanthones as promising antioxidants and disclosed as hit, 1,2-dihydroxyxanthone (1). Herein, we synthesized and studied the potential of xanthones with different polyoxygenated patterns as skin antiphotoaging ingredients. In the DPPH antioxidant assay, two newly synthesized derivatives showed IC50 values in the same range as ascorbic acid. The synthesized xanthones were discovered to be excellent tyrosinase inhibitors and weak to moderate collagenase and elastase inhibitors but no activity was revealed against hyaluronidase. Their metal-chelating effect (FeCl3 and CuCl2) as well as their stability at different pH values were characterized to understand their potential to be used as future cosmetic active agents. Among the synthesized polyoxygenated xanthones, 1,2-dihydroxyxanthone (1) was reinforced as the most promising, exhibiting a dual ability to protect the skin against UV damage by combining antioxidant/metal-chelating properties with UV-filter capacity and revealed to be more stable in the pH range that is close to the pH of the skin. Lastly, the phototoxicity of 1,2-dihydroxyxanthone (1) was evaluated in a human keratinocyte cell line and no phototoxicity was observed in the concentration range tested.

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In-vitro evaluation of antioxidant, anti-elastase, anti-collagenase, anti-hyaluronidase activities of safranal and determination of its sun protection factor in skin photoaging

Cited by 84 publications

References 35 publications

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Anti-elastase and anti-hyaluronidase activity of phosvitin isolated from hen egg yolk

Efficacy, Stability, and Safety Evaluation of New Polyphenolic Xanthones Towards Identification of Bioactive Compounds to Fight Skin Photoaging

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