In vitro evaluation of antioxidant and cytotoxic activity of folate-methotrexate conjugated to bovine serum albumin nanoparticles against MCF-7, HepG2, and PC3 cell lines

Al-Rahim, Aya M.; Mahmood, Rana I.; Mohammed, Mais M.; Omer, Dania

doi:10.1016/j.genrep.2022.101666

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…The cytotoxicity of these substrates was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [ 31 , 32 ]. MCF-7 cells were seeded in 96-well plates at 5000 cells per well using Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum.…”

Section: Methodsmentioning

confidence: 99%

Mg-Doped ZnO Nanoparticles with Tunable Band Gaps for Surface-Enhanced Raman Scattering (SERS)-Based Sensing

et al. 2022

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Semiconductors have great potential as surface-enhanced Raman scattering (SERS) substrates due to their excellent physiochemical properties. However, they provide low signal enhancements relative to their plasmonic counterparts, which necessitates innovation in their synthesis and application. Substitutional atomic doping is proposed to improve SERS enhancement by controlling electronic properties, such as the band gap. In this work, zinc oxide (ZnO) nanoparticles were synthesized by co-precipitation and doped with magnesium (Mg) at concentrations ranging from 2–10%. Nanoparticle morphology and size were obtained by scanning electron microscopy (SEM). Elemental composition and chemical states were determined using X-ray photoelectron spectroscopy (XPS). Optical properties were obtained with a UV-vis spectrophotometer, while a Raman spectrometer was used to acquire Raman signal enhancements. Stability was assessed by UV-vis spectroscopy, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that the absorption edge of Mg-doped ZnO nanoparticles was red-shifted compared to pure ZnO nanoparticles. The band gap decreased (3.3–3.01 eV) with increasing Mg doping, while the highest Raman enhancement was observed at 2% doping. No significant cytotoxic effects were observed at low concentrations (3–12 μg/mL). Overall, this study provides evidence for the tunability of ZnO substrates and may serve as a platform for applications in molecular biosensing.

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Section: Methodsmentioning

confidence: 99%

Mg-Doped ZnO Nanoparticles with Tunable Band Gaps for Surface-Enhanced Raman Scattering (SERS)-Based Sensing

et al. 2022

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“…Nanotechnology pertains to the use of material at the nanoscale level. Present studies in the domain of nanotechnology are drawing the attention of large firms and nations for investing in this swiftly growing field (10,11) . Material at nanometre scale make the most of novel physical, biological, and chemical attributes, which could alter or improve drug properties.…”

Section: Introductionmentioning

confidence: 99%

Efficient Delivery of Recombinant Human Bone Morphogenetic Protein (Rhbmp-2) With Cockle Shell Derived Calcium Carbonate Nanoparticles (CaCO3NPs)

T. Ghazi,

F. Saloom,

I. Mahmood

2024

TJDS

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Bone morphogenetic protein-2 (BMP-2) has a significant function in the formation of cartilage and bones. Notably, dosing of only BMP-2 protein intravenously is ineffective. Persistent transportation of the stabilized BMP-2 through a carrier has been seen to be essential for enhancing the osteogenesis im pact of BMP-2. The current research built a new system of drug delivery by utilising cockle shell derived calcium carbonate nanoparticles (CaCO3NPs) and studied the efficacy of the delivery system on the recombinant human bone morphogenetic protein (rhBMP-2). rhBMP-2-CaCO3NPs nanoparticles were synthesised by means of a modest precipitation procedure along with mechanical grinding. Fourier-tran sform infrared spectroscopy, UV–Vis spectrophotometer, scanning electron microscope, X-ray powder diffraction, transmission electron microscope, and zeta potential were u tilised for characterising the conjugated rhBMP-2-CaCO3NPs . Cytotoxicity of rhBMP-2, CaCO3NPs and rhBMP-2-CaCO 3NPs was studied by utilising methylthiazol tetrazolium assay against fibroblast (Rat-1) cells in comparison to rhBMP-2 and CaCO3NPs. The outcomes signified bio-stability of CaCO3NPs and lower toxicity for Rat-1 cells. In summary, CaCO3NPs were prepared by a simp le precipitation process. The ensuing nanoparticles could competently entrap rhBMP-2 and generated stable rhBMP-2-CaCO3NPs. A sustained discharge of rhBMP-2 from t he CaCO3NPs was seen. CaCO3NPs loaded with r hBMP-2 demonstrated reasonable bio-compatibility. The outcomes indicated that CaCO3NPs may have significant ability as carrier of therapeutic proteins within bone tissue en gineering.

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Nanocarriers for methotrexate delivery/codelivery in the frame of cancer diagnostics and treatment: a review

Mukhtar,

Ezra Manicum,

Shojaei Barjouei

et al. 2023

Front. Biomater. Sci.

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Cancer is one of the most life-threatening family of diseases that cause death worldwide. As a highly researched and successful therapeutic agent, methotrexate (MTX) treats many solid tumours, hematologic malignancies, and autoimmune illnesses. Despite many benefits, methotrexate induces drug resistance and limits plasma half-life due to its poor pharmacokinetics. The variable biological availability have prompted researchers to investigate innovative delivery strategies for enhancing its therapeutic qualities. To develop more suitable methotrexate formulations, nanoparticles (NPs) have recently gained a significant interest. A wide range of nanoparticles, including polymer-based nanoparticles, carbon-based nanoparticles, lipid-based nanoparticles, as well as inorganic nanoparticles, can be deliver cancer chemotherapeutics such as methotrexate. Loading methotrexate into NPs can provide a delivery system that has shown great promise to carcinoma therapy. In this review, we will describe the feasibility of NP-based strategies to deliver methotrexate in cancer therapy, outlining the current state of the art and the challenges/promises for the future.

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In vitro evaluation of antioxidant and cytotoxic activity of folate-methotrexate conjugated to bovine serum albumin nanoparticles against MCF-7, HepG2, and PC3 cell lines

Cited by 3 publications

References 21 publications

Mg-Doped ZnO Nanoparticles with Tunable Band Gaps for Surface-Enhanced Raman Scattering (SERS)-Based Sensing

Mg-Doped ZnO Nanoparticles with Tunable Band Gaps for Surface-Enhanced Raman Scattering (SERS)-Based Sensing

Efficient Delivery of Recombinant Human Bone Morphogenetic Protein (Rhbmp-2) With Cockle Shell Derived Calcium Carbonate Nanoparticles (CaCO3NPs)

Nanocarriers for methotrexate delivery/codelivery in the frame of cancer diagnostics and treatment: a review

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