In vitro evaluation of antimicrobial and cytotoxic potential of Epimedium grandiflorum hydroethanolic extract as natural medicine

Abstract

Medicinal plants are used as fundamental and low-cost source for remedy of numbers of infectious and metabolic diseases in developing and developed countries. Current research work was planned to evaluate the antibacterial, antifungal, and cytotoxic potential of hydroethanolic extract of E. grandiflorum. It was found that selected natural medicinal herb have significant (p<0.05) antibacterial activities tested against Bacillus subtilis, Staphylococcus aureus, Pasteurella multocida, Escherichia coli, Klebsiella pneumoniae, Acinetobacter species, Pseudomonas Species and Salmonella Species. The results of bacterial biofilm inhibition also explored that selected natural herb has significant (p<0.05) capacity to prevent the microbial biofilm particularly at higher dose. The results of antifungal activities showed that selected medicinal plant has significant (p<0.05) antifungal potential evaluated against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Fusarium solani, Alternata alternaria, and Schizophyllum species. Moreover, the results of mutagenicity test and DNA damage preventive test explored that selective medicinal plant has significant (p<0.05) DNA protective capacity or in other words it is non-mutagenic or cytotoxic in nature. It could be concluded that E. grandiflorum could be a potential candidate as therapeutic agent to manage infectious diseases especial bacterial and fungal infections with non-toxic nature.

Study of the Biological Activity of Microwave Synthesized of some new Pyridine derivatives fused with Sulfonamide Moiety

“…Amoxycillin is used as standard to detect antibacterial activity of Compounds 2a, 3a, 4b, 15a and 18b by well diffusion method [21,22] . The tested compounds 2a, and 15a showed the highest activity while Compounds 3a, 18b and 4b showed medium activity than Amoxycillin against Gram positive and Gram negative.…”