In-Vitro Evaluation of Antifungal Activity of Terbinafine and Terbinafine Nano-Drug Against Clinical Isolates of Dermatophytes

Motedayen, Nazila; Hashemi, Seyed Jamal; Rezaei, Shahla; Bayat, Mansour

doi:10.5812/jjm.62351

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…Similarly, Motedayen et al (2018) evaluated the antifungal activity of terbinafine and terbinafine nano-drug against clinical isolates of dermatophytes and demonstrated that the MIC values of nano-liposomal terbinafine against T. rubrum and M. canis were 0.0156 to 0.25 μ/mL and 0.0078 to 0.125 μ/mL, respectively. In addition, MIC values of terbinafine against T. rubrum and M. canis strains were 0.0625 to 1 μ/mL and 0.0313 to 0.5 μ/mL, respectively (29). That study showed that the nanoparticles of terbinafine had a greater antifungal activity against the T. rubrum and M. canis isolates in comparison with free terbinafine, which is in line with our findings.…”

Section: Discussionsupporting

confidence: 90%

Effect of fluconazole and terbinafine nanoparticles on the treatment of dermatophytosis induced by Trichophyton mentagrophytes in guinea pig

Lalvand

Hashemi

Bayat

2021

IJM

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Background and Objectives: Dermatophytosis induced by Trichophyton mentagrophytes is a major human and animal fun- gal contamination. Antifungals like terbinafine and fluconazole are widely used to treat dermatophytosis; nevertheless, the prevalence of drug resistance has increased. Hence, novel curative strategies are needed. In the present study, we compared the efficacies of conventional and nanoform of antifungals agents in guinea pig model of dermatophytosis. Materials and Methods: Guinea pigs (n=36) were injected (the posterior dorsal portion) with Trichophyton mentagrophytes conidia. The guinea pigs were divided into 6 groups (positive control, negative control, fluconazole 0.5% treated group, na- no-fluconazole treated group, terbinafine 1% treated group, and nano-terbinafine treated group), then were scored both clin- ically (redness and lesion intensity) and mycologically (microscopy and culture) until day 40 of inoculation. The treatment started 5 days after the inoculation and continued until day 40 of inoculation. Results: Assessment of the mean score of clinical lesions in groups treated with nano-drug forms of fluconazole and terbin- afine on the first day of treatment showed a score of 3 (significant redness with large scaling) and for the conventional form of terbinafine and fluconazole had a score of 4 (ulcer and scar). The decrease in lesion score in nano-drug treated groups was observed between days 15 and 20 and continued until day 40. On day 40, all groups had zero scores except the positive control group. Conclusion: This study indicated that nano-drugs are more suitable for the treatment of dermatophytosis and could be con- sidered as future alternatives for the treatment of dermatophytosis.

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Section: Discussionsupporting

confidence: 90%

Effect of fluconazole and terbinafine nanoparticles on the treatment of dermatophytosis induced by Trichophyton mentagrophytes in guinea pig

Lalvand

Hashemi

Bayat

2021

IJM

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“…Multi-drug resistance results primarily from increased expression of genes belonging to the superfamily of ATP-binding cassettes, and primarily ABC transporters, which occur in both prokaryotes and eukaryotes (El-Awady et al 2016; Monod et al 2019). The ABC transporters actively transfer many structurally and chemically diverse compounds across cell membranes through ATP binding and hydrolysis, thereby reducing their accumulation in the cell (Sharma and Prasad 2011;Motedayen et al 2018).…”

Section: Mechanisms Of Resistance To Antifungal Drugsmentioning

confidence: 99%

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

2020

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Dermatophytes are the aetiological factors of a majority of superficial fungal infections. What distinguishes them from other pathogenic filamentous fungi is their unique ability to degrade keratin. The remarkable ability of this group of fungi to survive in different ecosystems results from their morphological and ecological diversity as well as high adaptability to changing environmental conditions. Paradoxically, despite the progress in medicine, the prevalence of dermatophyte infections is increasing from year to year. At the beginning of the third millennium, practical diagnostic and therapeutic options are still very limited. This review focuses on understanding the major problems in this aspect of dermatophyte infections and indicates future strategies and perspectives for novel approaches to identification and drugs for elimination of dermatophytes. Particular importance is placed on development of a strategy for a diagnostic pathway and implementation of rapid and reliable diagnostics methods designed by international teams. Furthermore, among compounds that currently arouse great interest, representatives of terpenoids, alkaloids, saponins, flavonoids and essential oils deserve attention. Many of these compounds are undergoing clinical trials as potential antifungal agents, and future research should focus on attempts at determination of the applicability of tested substances. Finally, the advantages and disadvantages in implementation of new diagnostic paths and medicinal substances for routine use are indicated. Journal of Applied Microbiology

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“…The fungal colonies were covered with 2 mL of Sabouraud’s dextrose broth (SDB) followed by a gentle scratching of the agar’s surface with a sterile swab, resulting in a suspension of conidial and hyphal fragments that was transferred to a sterile tube and kept for 5 to 10 minutes at room temperature for sedimentation of heavy particles keeping micro-conidia suspended. Filtration was performed to retain hyphal fragments but permit the passage of dermatophyte micro-conidia to be used later (Motedayen et al., 2018 ). The turbidity of the fungal suspension was measured spectrophotometrically (ultraviolet/visible [UV/VIS] spectrophotometer; JASCO, Tokyo, Japan) at 600 nm and adjusted with SDB to match the 0.5 McFarland standard which is equivalent to 10 5 -10 6 CFU/mL (Marcato et al., 2012 ).…”

Section: Methodsmentioning

confidence: 99%

“…Plates were incubated at 28 °C for 4 days. Results were evaluated visually (Motedayen et al., 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Hydroxypropyl chitosan nail lacquer of ciclopirox-PLGA nanocapsules for augmented in vitro nail plate absorption and onychomycosis treatment

2022

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Onychomycosis accounts for 90% of nail infections worldwide. Topical therapy provides localized effects with minimal adverse systemic actions, yet its effectiveness is limited by minimal drug permeation through the keratinized nail plate. Ciclopirox (CIX) is a FDA-approved broad-spectrum antimycotic agent. However, the complete cure with its nail lacquer (8% w/v) may continue for one year with a high cost. Therefore, poly lactide-co-glycolide (PLGA) nanocapsules (NCs) of CIX were prepared by nanoprecipitation and optimized through a 2 3 factorial design to be incorporated into hydroxypropyl chitosan (HPCH) based nail lacquer. Nail hydration, in vitro nail absorption, minimum inhibitory concentration (MIC), inhibition zones and ex vivo fungal growth on nail fragments were evaluated. The optimized NCs of CIX based on 100 mg PLGA 2 A and lipoid S75 showed a mean diameter of 174.77 ± 7.90 nm, entrapment efficiency (EE%) of 90.57 ± 0.98%, zeta potential (ZP) of −52.27 ± 0.40 mV and a prolonged drug release. Nail lacquer of the optimized NCs exhibited a higher stability than NCs dispersion. Compared to CIX solution (1% w/v), the respective decrease in MIC for NCs and their lacquer was four- and eight-fold. The lacquer superiority was confirmed by the enhancement in the nail hydration and absorption by 4 and 2.60 times, respectively, relative to CIX solution and the minimal ex vivo fungal growth. Therefore, HPCH nail lacquer of (1% w/v) CIX-PLGA-NCs can be represented as a potential topical delivery system for enhanced in vitro nail absorption and therapeutic efficacy against onychomycosis at a low dose.

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In-Vitro Evaluation of Antifungal Activity of Terbinafine and Terbinafine Nano-Drug Against Clinical Isolates of Dermatophytes

Cited by 9 publications

References 19 publications

Effect of fluconazole and terbinafine nanoparticles on the treatment of dermatophytosis induced by Trichophyton mentagrophytes in guinea pig

Effect of fluconazole and terbinafine nanoparticles on the treatment of dermatophytosis induced by Trichophyton mentagrophytes in guinea pig

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

Hydroxypropyl chitosan nail lacquer of ciclopirox-PLGA nanocapsules for augmented in vitro nail plate absorption and onychomycosis treatment

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