In vitro evaluation of anticancer effect and neurotoxicity of Styrylpyrone derivative (SPD)

Yip, Chee Wai; Nagaoka, Yasuo; Nor, Norefrina Shafinaz Md; Ibrahim, Nazlina

doi:10.1063/1.4966723

Cited by 4 publications

(4 citation statements)

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“…Salah satu spesies yang mengandungi goniothalamin ialah Goniothalamus umbrosus J. Sinclair yang juga digelar sebagai kenerak. Goniothalamin yang terbukti mempunyai aktiviti antikanser (Inayat-Hussain et al, 2010;Seyed et al, 2014;Yip et al, 2016), aktiviti antivirus (Abdul-Wahab et al, 2011;Md Nor & Ibrahim, 2012;Abdul Wahab & Ibrahim, 2020;Ibrahim et al, 2022) dan sebagainya. Konfigurasi yang sering dipencilkan secara semulajadi adalah dalam bentuk trans (Ahmad et al, 2010).…”

Section: Sebatianunclassified

Goniothalamin Berkesan Secara In Vitro Terhadap Virus Herpes Manusia Jenis 1 Rintang-Acyclovir

Hussin

Nor

Bahtiar

et al. 2022

MAB

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Permasalahan besar yang dihadapi dalam rawatan jangkitan virus herpes manusia jenis I (Human Herpes virus type 1, HHV-1) adalah kehadiran virus mutan yang rintang ubat antivirus iaitu acyclovir. Pencarian ubatan dengan kebolehan mengatasi HHV-1 rintang-acyclovir diperlukan bagi mengatasi masalah ini. Maka, kajian ini bertujuan menentukan konfigurasi Goniothalamin (GTN) yang dipencilkan daripada Goniothalamus umbrosus seterusnya mengenalpasti kebolehannya secara in vitro merencat pencilan HHV-1 mutan yang rintang-acyclovir. Kristal yang terhasil selepas pengekstrakan bahagian akar dan batang G. umbrosus disahkan melalui GC-MS, FTIR dan NMR sebagai GTN. Kiraan puncak gandingan J (J coupling peak calculation) menunjukkan bahawa sebatian aktif ini dalam konfigurasi-cis. Sebelum ujian antivirus dilakukan, nilai kepekatan sitotoksik GTN yang membunuh 50% populasi sel Vero (CC50) ditentukan iaitu 8.747 µg/mL. Kepekatan GTN lebih rendah daripada nilai CC50 digunakan untuk penentuan kesan aktiviti antivirus. Sebanyak sebelas pencilan mutan digunakan bagi menentukan kesan aktiviti antivirus GTN dalam asai pasca-rawatan. Nilai kepekatan berkesan menurunkan 50% pembentukan plak (EC50) adalah antara 0.47 µg/mL (2.35 µM) hingga 1.42 µg/mL (6.42 µM). Nilai indeks pemilihan (SI) iaitu nisbah keberkesanan aktiviti antivirus berbanding kesitotoksikan adalah antara 6 hingga 17 yang menunjukkan bahawa GTN mempunyai potensi baik untuk bertindak sebagai agen antivirus. Kesimpulannya, GTN dalam konfigurasi cis berkesan merencat pencilan virus mutan rintang-acyclovir. Potensi tindakan tersebut penting dalam menangani kehadiran virus rintang-acyclovir terutama yang mempunyai mutasi pada timidin kinase atau polimerase DNA.

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Section: Sebatianunclassified

Goniothalamin Berkesan Secara In Vitro Terhadap Virus Herpes Manusia Jenis 1 Rintang-Acyclovir

Hussin

Nor

Bahtiar

et al. 2022

MAB

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“…This structure becomes the core skeleton for other types of styryl-lactones such as furano-pyrones and pyrano-pyrones [ 30 ]. This phytochemical compound has been reported to have potent anti-viral activity against herpes simplex virus type 1 [ 31 , 32 ], an anti-cancer effect, and neurotoxicity [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…has been reported to have potent anti-viral activity against herpes simplex virus type 1 [31,32], an anti-cancer effect, and neurotoxicity [33]. The identification of a pure compound can be determined using gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR).…”

Section: Introductionmentioning

confidence: 99%

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Wahab

Ibrahim

2022

Molecules

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A study was conducted to investigate the anti-viral effect of a styrylpyrone derivative (SPD) called goniothalamin and the effects on the dengue virus serotype 2 (DENV-2) replication cycle. The SPD was prepared from the root of Goniothalamus umbrosus after purification with petroleum ether. The isolated SPD was then subjected to gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses for structure validation. The cytotoxicity of the SPD was evaluated using a cell viability assay, while the anti-viral activity of the SPD towards DENV-2 was confirmed by conducting a foci reduction assay which involved virus yield reduction, time-of-addition, and time removal assays. Transcriptomic analysis via quantitative real-time polymerase chain reaction (qRT-PCR) using the DENV-2 E gene was conducted to investigate the level of gene transcript. Immunocytochemistry analysis was used to investigate the effects of SPD treatment on protein E expression. Finally, software molecular docking of the SPD and E protein was also performed. The cytotoxicity assay confirmed that the SPD was not toxic to Vero cells, even at the highest concentration tested. In the time-of-addition assay, more than 80% foci reduction was observed when SPDs were administered at 2 h post-infection (hpi), and the reduction percentage then dropped with the delay of the treatment time, suggesting the inhibition of the early replication cycle. However, the time removal assay showed that more than 80% reduction could only be observed after 96 h post-treatment with the SPD. Treatment with the SPD reduced the progeny infectivity when treated for 24 h and was dose-dependent. The result showed that transcript level of the E gene in infected cells treated with the SPD was reduced compared to infected cells without treatment. In immunocytochemistry analysis, the DENV-2 E protein exhibited similar expression trends, shown by the gene transcription level. Molecular docking showed that the SPD can interact with E protein through hydrogen bonds and other interactions. Overall, this study showed that SPDs have the potential to be anti-DENV-2 via a reduction in viral progeny infectivity and a reduction in the expression of the DENV-2 E gene and protein at different phases of viral replication. SPDs should be further researched to be developed into an effective anti-viral treatment, particularly for early-phase dengue viral infection.

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“…This structure becomes the core skeleton for other types of styryl-lactones such as furanowww.videleaf.com pyrones and pyrano-pyrones [30]. This phytochemical compound has been reported to have potent anti-viral activity against herpes simplex virus type 1 [31,32], an anti-cancer effect, and neurotoxicity [33]. The identification of a pure compound can be determined using gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR).…”

Section: Introductionmentioning

confidence: 99%

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Abd Wahab,

Ibrahim

2022

Prime Archives in Molecular Sciences

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In vitro evaluation of anticancer effect and neurotoxicity of Styrylpyrone derivative (SPD)

Cited by 4 publications

References 12 publications

Goniothalamin Berkesan Secara In Vitro Terhadap Virus Herpes Manusia Jenis 1 Rintang-Acyclovir

Goniothalamin Berkesan Secara In Vitro Terhadap Virus Herpes Manusia Jenis 1 Rintang-Acyclovir

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

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