In Vitro Evaluation of Antibacterial Chemicals and Bioagents against Ralstonia solanacearum Infecting Bacterial Wilt in Ginger

Singh, Roop; Jagtap, G. P.

doi:10.20546/ijcmas.2017.605.227

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Whereas, bleaching powder was less effective even at higher concentrations. These results are in conformity with the findings of those reported earlier by several workers (Sunder et al, 2011;Gupta and Razdan, 2013;Owoseni and Sangoyomi, 2014, Raghu et al, 2011and Roop Singh, 2017.…”

Section: Resultssupporting

confidence: 93%

“…Literature supported for the in vitro evaluation, under biological control of plant diseases, various antagonistic organisms have been identified, which fight against the pathogens by different mechanisms viz., competition, lysis, antibiosis, siderophore production and hyper parasitism (Vidyasekaran, 1999). Bioagents viz., P. fluorescens and B. subtilis were reported efficient antagonists against R. solanacearum earlier by many workers (Gupta and Razdan, 2013;Raghu et al, 2011 andRoop Singh, 2017 Data from the table revealed that, the efficacy of different bactericides; concentrations and their interaction on formation of inhibition zone differed significantly tabulate in Table 3 and Plate 3. Among different chemicals and their combinations, streptocycline + copper oxychloride at 500 ppm + 3000 ppm showed the highest inhibition of 29.38 mm followed by Streptocycline 500 ppm (27.07 mm).…”

Section: Resultsmentioning

confidence: 87%

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In vitro Evaluation of Botanicals, Bioagents and Antibacterial Chemicals against Rhizome Rot of Ginger Caused by Ralstonia solanacearum

Nagaraja¹,

Murali²,

Narayanaswamy³

2018

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 87%

In vitro Evaluation of Botanicals, Bioagents and Antibacterial Chemicals against Rhizome Rot of Ginger Caused by Ralstonia solanacearum

Nagaraja¹,

Murali²,

Narayanaswamy³

2018

Int.J.Curr.Microbiol.App.Sci

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“…Paul (1998) observed that 250 and 500 ppm concentrations of ambistryn, oxytetracycline, and streptocycline inhibited R. solanacearum in vitro. Singh and Jagtap (2017) also documented the effectiveness of antibacterial chemicals and bioagents against the in vitro growth of R. solanacearum. The average inhibition ranged from 6.2 mm (copper hydroxide) to 20.05 mm (Streptocycline).…”

Section: Discussionmentioning

confidence: 99%

Effect of antibiotics in eliminating bacterial wilt (Ralstonia solanacearum) from in vitro propagated ginger

MARKOS

Feyissa

2020

Cell Biol Dev

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Abstract. Markos T, Feyissa T. 2020. Effect of antibiotics in eliminating bacterial wilt (Ralstonia solanacearum) from in vitro propagated ginger. Cell Biol Dev 4: 46-52. Ginger (Zingiber officinale Roscoe) is a herbaceous perennial. It is cultivated commercially in most tropical regions of the world and is a member of the Zingiberaceae family. In conventional ginger cultivation, underground rhizomes are used as planting material. However, it is afflicted by diseases that result in the senescence and degeneration of tissues. Due to Ralstonia solanacearum infection, substantial rhizome losses have been recorded. Therefore, disease-free planting material is required to cultivate ginger successfully. Plant tissue culture technology has been successfully used to commercialize pathogen-free plants and conserves the germplasm of rare and endangered species. In in-vitro, culture techniques offer an alternative method of plant multiplication and a method for crop enhancement. Frequently, ginger multiplication media are contaminated with R. solanacearum, which can survive endophytically in plantlets, rendering them useless for in vitro propagation. Therefore, an experiment was undertaken to determine the efficiency of antibiotics against the in vitro development of R. solanacearum. Four antibiotics, gentamicin, tetracycline, ampicillin, and streptomycin, at four concentrations, 130 mg/L, 160 mg/L, 200 mg/L, and 250 mg/L, were evaluated for their ability to eradicate bacteria from in-vitro propagated ginger. Gentamicin was proven superior to other therapies, with the maximum inhibition (22 mm) at 250 mg/L, followed by tetracycline at 200 mg/L (18 mm), streptomycin at 130 mg/L (13 mm), and ampicillin at 130 mg/L (12 mm). The R. solanacearum can be eradicated from in vitro propagated ginger by applying antibiotics in the micropropagation media.

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“…Several studies reported that the antagonist bacteria Pseudomonas fluorescens and Bacillus subtilis were effective and efficient in suppressing the growth of R. solanacearum bacteria in vitro (Singh & Jagtap, 2017). Some of the antagonistic bacteria were used to control bacterial wilt disease that caused by the pathogen R. solanacearum (Table 2).…”

Section: Antagonistic Bacteria As Biocontrol Of Ralstonia Solanacearummentioning

confidence: 99%

Acreditation Certificate Acreditation No. 21/E/KPT/2018 Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Naziha Diyanatur Rosiyah Brawijaya University Indonesia Study Program Agroecotechnology Department Plant Pes and Disease Ayu Wike Widiasari Brawijaya University Indonesia Study Program Agroecotechnology Department Plant Pes and Disease Achmad Roekhan Brawijaya University Indonesia Study Program Agroecotechnology Department Pl

Rosiyah

Widiasari

Roekhan

et al. 2020

RJLS

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Ginger (Zingiber officinale Rosc.) is a type of rhizome plant that has high potential to be developed in Indonesia as a medicinal plant or spice. Inhibiting factors in ginger production is Ralstonia solanacearum which causes bacterial wilt disease. Rhizobacteria is a potential biological agent to control the disease. It is necessary to review from various study on rhizobacteria both the use of antagonistic bacteria singly or in a consortia. Aimed to analyze various sources of scientific literature regarding the potential of antagonistic bacteria singly or consortia compared to synthetic bactericides in controlling of R. solanacearum. The results showed that three recommended antagonist bacteria were found, namely Pseudomonas fluorescens, Bacillus subtilis, and Bacillus thuringiensis. which has the potential to control bacterial wilt disease. The rhizobacterial consortia are more effective as a sustainable, safe and environmentally friendly control on bacterial wilt disease with a percentage of disease incidence 10%. In addition, the rhizobacterial consortia are three and six times more effective than the single antagonistic bacteria and bactericides respectively.

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In Vitro Evaluation of Antibacterial Chemicals and Bioagents against Ralstonia solanacearum Infecting Bacterial Wilt in Ginger

Cited by 5 publications

References 12 publications

In vitro Evaluation of Botanicals, Bioagents and Antibacterial Chemicals against Rhizome Rot of Ginger Caused by Ralstonia solanacearum

In vitro Evaluation of Botanicals, Bioagents and Antibacterial Chemicals against Rhizome Rot of Ginger Caused by Ralstonia solanacearum

Effect of antibiotics in eliminating bacterial wilt (Ralstonia solanacearum) from in vitro propagated ginger

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