In vitro evaluation of anti-pathogenic surface coating nanofluid, obtained by combining Fe3O4/C12 nanostructures and 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides

Abstract

In this paper, we report the design of a new nanofluid for anti-pathogenic surface coating. For this purpose, new 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides were synthesized and used as an adsorption shell for Fe3O4/C12 core/shell nanosized material. The functionalized specimens were tested by in vitro assays for their anti-biofilm properties and biocompatibility. The optimized catheter sections showed an improved resistance to Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 in vitro biofilm development, as demonstrated by the viable cell counts of biofilm-embedded bacterial cells and by scanning electron microscopy examination of the colonized surfaces. The nanofluid proved to be not cytotoxic and did not influence the eukaryotic cell cycle. These results could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with improved anti-biofilm properties.

Synthesis, cytotoxicity and antimicrobial activity of thiourea derivatives incorporating 3-(trifluoromethyl)phenyl moiety

“…derivatives (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and N-alkyl- [3-(trifluoromethyl)phenyl]thiourea derivatives (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31) M A N U S C R I P T…”

Potential Application of Nanoparticles as Antipathogens

“…Th is was demonstrated by the viable cell counts of biofi lm-embedded bacterial cells and by scanning electron microscopy examination of the colonized surface. Th e nanofl uid proved to be noncytotoxic and did not infl uence the eukaryotic cell cycle [64].…”

Silver nanoparticles embedded into silica functionalized with vitamins as biological active materials

“…The assessment of the complexes influence on the microbial ability to colonize an inert substratum was performed by the microtiter method, following previously described protocols [34][35][36][37] …”