In Vitro Evaluation of Acellular Dermal Matrix as a Three‐Dimensional Scaffold for Gingival Fibroblasts Seeding

Maia, Luciana Prado; Novaes, Arthur B.; Souza, Sérgio L. S. de; Grisi, Márcio F. M.; Taba, Mário; Palioto, Daniela Bazan

doi:10.1902/jop.2010.100121

Cited by 14 publications

(17 citation statements)

References 45 publications

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“…On the other hand, significantly higher values of total cell number, cell viability and total protein content were noted for HGF. These results corroborate with those of a recent study (13), in which HGF showed higher cell viability than CGF when seeded on a three-dimensional scaffold.…”

Section: Discussionsupporting

confidence: 92%

“…Some authors have been evaluating non-enzymatic methods of gingival fibroblasts isolation in vitro for the use in gingival augmentation (6,(10)(11)(12)(13)(14). Gingival fibroblast culture in different matrices has been studied and has shown promising results in soft tissues regeneration (12)(13)(14). Fibroblasts are responsible for the normal growth of the organism and are essential in cases where tissue repairs are necessary, being one of the first cells to appear at injured sites.…”

Section: Discussionmentioning

confidence: 99%

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Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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“…As fibroblasts have stem‐cell‐like properties and can differentiate to osteoblast‐like cells and have the ability to secret calcified matrix, fibroblasts recently have been utilized as a seeding cell for tissue regeneration for bone and periodontal reconstruction (e.g. ridge augmentation and in tissue‐guided regeneration) . In our present study, our results indicated that HGF cell line produced ALP enzyme with a lower activity after exposure to certain concentrations of caffeine (Figure ).…”

Section: Discussionsupporting

confidence: 59%

“…In addition, fibroblasts are capable of osteoblastic differentiation and possess unique mesenchymal stem‐cell‐like properties with osteogenic and fibrogenic capacity . As gingival fibroblasts share many osteoblast‐like properties, for example, high alkaline phosphatase (ALP) activity, expression of bone associated proteins, and formation of mineralized nodules in in vitro studies, they have been used extensively in the past few years in therapeutic procedures for periodontal tissue regeneration therapy and preprosthetic, pre‐implantology surgeries …”

Section: Introductionmentioning

confidence: 97%

Caffeine alters mitochondrial dehydrogenase and alkaline phosphatase activity of human gingival fibroblasts in vitro

Bozchaloei

Gong

Dehpour

et al. 2012

J of Invest & Clin Dent

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“…Los tejidos del donante se preparan mediante la eliminación de la epidermis y los componentes celulares de la piel reduciendo así la posibilidad de rechazo sobre el injerto. La MDA es entonces crioprotegida y rápi-damente liofilizada, esto para preservar su integridad estructural y bioquímica (13)(14)(15), finalmente aumenta el espesor del tejido y a partir de un punto de vista histológico se adhiere a los tejidos periodontales de forma similar al tejido conectivo y sus cualidades no inmunogénicas hacen que sea viable el trasplante dermal, manteniendo la integridad estructural y la revascularización (3,13).…”

Section: Introductionunclassified

Comparación de dos técnicas en el manejo quirúrgico de la recesión radicular utilizando matriz dérmica acelular: reporte de un caso

Membreño

2014

Av Odontoestomatol

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Juárez Membreño I. Comparación de dos técnicas en el manejo quirúrgico de la recesión radicular utilizando matriz dérmica acelular. Reporte de un casoAVANCES EN ODONTOESTOMATOLOGÍA/187 RESUMENSe han descrito, en cirugía plástica periodontal, diferentes procedimientos y materiales quirúrgicos con el propósito del tratamiento de recesión gingival (RG), el incremento del espesor y ancho de encía adherida. Uno de los materiales empleados es la matriz dérmica acelular (MDA), aloinjerto de base biológica que de forma similar al tejido conectivo se incorpora a los tejidos periodontales, manteniendo la integridad estructural y la revascularización. Las técnicas para MDA puede ser un colgajo desplazado coronal (CDC) con incisiones verticales o la técnica quirúrgica en sobre o túnel modificado mediante el levantamiento desde el interior de las papilas permitiendo mayor movilización coronal del tejido y un desplazamiento coronal del colgajo. El objetivo del reporte de caso fue comparar las dos técnicas anteriores con un seguimiento de seis meses. El caso clínico demostró concordancia con la evidencia de los estudios científicos que afirma éxito para tratar cobertura de recesiones con ganancia de tejido queratinizado, aumento de espesor de encía adherida, mejorías clínicas y resultados estéticos independiente de la técnica quirúrgica empleada en el uso de MDA. Palabras clave: Matriz dérmica acelular (MDA), recesión gingival (RG), colgajo desplazado coronal (CDC), cobertura radicular (CR). SUMMARYDifferent surgical procedures and materials have been described with the purpose of carrying out gingival recession treatments (GR), the increase of thickness and width of adhered gum. One of the materials used is the acellular dermal matrix (ADM) biological base allograft which, like a connective tissue, is added to the periodontal tissues, keeping the structural integrity and re vascularization. The ADM techniques can be either a coronal displaced flap (CDF) with vertical incisions or a surgical technique in a kind of pocket or tunnel modified by means of a lifting from inside the papillae allowing a higher tissue coronal movement and a flap coronal displacement. The case report objective was to compare both techniques with a post six-month follow up. The clinical case showed concordance with the evidence of scientific studies which confirms the success for treating recession cover with a gain of keratinized tissue, increase of added gum thickness, better clinical improvements and aesthetic results regardless the surgery technique used when using ADM.

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In Vitro Evaluation of Acellular Dermal Matrix as a Three‐Dimensional Scaffold for Gingival Fibroblasts Seeding

Abstract: ADM is not suitable as a three-dimensional matrix for gingival fibroblasts ingrowth. Gingival fibroblasts and highly proliferative cells as B16F10 can only be superficially located on ADM, and CGF are negatively affected by culture medium conditioned in ADM, reducing its viability.

Cited by 14 publications

References 45 publications

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

Caffeine alters mitochondrial dehydrogenase and alkaline phosphatase activity of human gingival fibroblasts in vitro

Comparación de dos técnicas en el manejo quirúrgico de la recesión radicular utilizando matriz dérmica acelular: reporte de un caso

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