In vitro Etest synergy of doripenem with amikacin, colistin, and levofloxacin against Pseudomonas aeruginosa with defined carbapenem resistance mechanisms as determined by the Etest method

He, Wendy; Kaniga, Koné; Lynch, A. Simon; Flamm, Robert K.; Davies, Todd A.

doi:10.1016/j.diagmicrobio.2012.08.014

Cited by 18 publications

(13 citation statements)

References 11 publications

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“…Then, this first Etest strip was removed and an Etest strip of the second antimicrobial agent was placed at the same location as the first strip. All tests were performed on Mueller–Hinton agar and incubated at 37 °C in an aerobic atmosphere for 16–20 h .…”

Section: Methodsmentioning

confidence: 99%

“…The fractional inhibitory index concentration (FIC) index was used to determine whether each specific combination of antibiotics resulted in a synergistic, additive, indifferent, or antagonistic effect. The FIC index values were interpreted as follows: FIC index ≤0.5 indicated a synergistic effect, FIC index >0.5 and ≤1.0 an additive effect, FIC index >1.0 and ≤4.0 an indifferent effect, and FIC index >4.0 an antagonistic effect .…”

Section: Methodsmentioning

confidence: 99%

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Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections, with focus on doxycycline

Hamad

Hellmark

Nilsdotter-Augustinsson

et al. 2015

APMIS

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In recent years, coagulase-negative staphylococci such as Staphylococcus epidermidis have gained importance as nosocomial pathogens, especially in immunocompromised patients and prosthetic joint infections (PJIs). These infections are often long lasting and difficult to treat due to the production of bacterial biofilm and the transformation of the bacteria into a stationary growth phase. Rifampicin is able to penetrate the biofilm, but to reduce the risk of development of rifampicin resistance it should be used in combination with an additional antibiotic. In this study we used Etest to investigate the antimicrobial susceptibility of 134 clinical isolates of S. epidermidis obtained from PJIs to six oral antibiotics: doxycycline, rifampicin, linezolid, fusidic acid, clindamycin, and ciprofloxacin. We also performed synergy testing on doxycycline in combination with each of the remaining antibiotics. Ninety-three (69%) of the 134 isolates were susceptible to doxycycline, 94/134 (70%) to rifampicin, 56/134 (42%) to clindamycin, 25/134 (19%) to ciprofloxacin, 81/134 (60%) to fusidic acid, and 100% to linezolid. Thirty-two (80%) of the 40 isolates not fully susceptible to rifampicin were susceptible to doxycycline. Doxycycline in combination with each of the other investigated antibiotics exerted an additive effect on nearly half of the isolates, with the exception of clindamycin, which displayed an even higher percentage of additive effect (69%). To conclude, as the majority of the S. epidermidis isolates were susceptible to doxycycline, this antimicrobial agent may provide a potential alternative for combination therapy together with rifampicin.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections, with focus on doxycycline

Hamad

Hellmark

Nilsdotter-Augustinsson

et al. 2015

APMIS

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“…Thus, in vitro synergy studies are important to define combination therapy that can be useful to treat these infections. To date, few studies evaluated antimicrobial synergy against CRPA (3 5) and the role of virulence on the high mortality associated with CRPA-BSI is controversial as well (2, 3).…”

Section: Introductionmentioning

confidence: 99%

Carbapenem-resistant Pseudomonas aeruginosa bloodstream infection: in vitro synergy, virulence and clinical outcome

Rizek

et al. 2018

Preprint

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34 Página2In the current study carbapanem-resistant P. aeruginosa bloodstream 35 infection (CRPA-BSI) was associated with high 14-day mortality. Although, 36 highly resistant to meropenem, half of the isolates achieved in vitro synergy 37 with the combination of meropenem with colistin, and the patients that 38 received this combination therapy showed a tendency towards lower mortality. 39Our strains did not carry exoU gene, on the other hand, patients with CRPA-40 BSI caused by strains harboring gene lasB evolved more frequent to death. 41 42

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“…The results of Ahmad et al (2014) study show that Staphylococcus aureus is resistant to cafepime antibiotics (93%) and ciprofloxacin (50%), and intermediates to neomycin (28.6%), streptomycin (85.7%) and amikacin ( 50%). Meanwhile, based on the results of the research He et al, (2012) Pseudomonas aerugynosa resistant to carbapenem class with 67% (doripenem-amikacin), 31% (doripenem-colistin), 23% (doripenem-levofloxacin).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of dialkyl-aginate biosurfactant cream againts Staphylococcus aureus an Pseudomonas aerugynosa

2018

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Dialkyl-alginate biosurfactant is an amphifilic rhamnolipid biosurfactant which has the potential to be developed into an antibacterial agent. The purpose of this study was to prove the biosurfactant of dialkyl alginate and it's cream formula as antibacterial activity especially against Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial activity assay of biosurfactant dialkyl alginate at concentrations of 5%, 10% and 20% againts Staphylococcus aureus and Pseudomonas aeruginosa using modified quantitative method. Enbatic® 1% is used as a positive control and sterile aquadest as a negative control. Determination of antibacterial activity of dialkyl alginate biosurfactant followed by analysis of leakage of protein and nucleic acids using UV-Vis Spectrophotometry and leakage of Ca 2+ and K + metal ions using Atomic Absorption Spetrophotometry (AAS). The most active concentration was formulated into cream and tested antibacterial activity using the well method. The test results showed that the biosurfactants of dialkyl-alginate and it's cream formula has antibacterial activity. The concentration of 10% was the most active concentration having activity which did not differ significantly to positive control with p value of 0,05.

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In vitro Etest synergy of doripenem with amikacin, colistin, and levofloxacin against Pseudomonas aeruginosa with defined carbapenem resistance mechanisms as determined by the Etest method

Cited by 18 publications

References 11 publications

Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections, with focus on doxycycline

Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections, with focus on doxycycline

Carbapenem-resistant Pseudomonas aeruginosa bloodstream infection: in vitro synergy, virulence and clinical outcome

Antibacterial activity of dialkyl-aginate biosurfactant cream againts Staphylococcus aureus an Pseudomonas aerugynosa

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