In Vitro Embryo Rescue of Interspecific Crosses for Transferring Virus Resistance in Okra (Abelmoschus Esculentus (L.) Moench)

Rajamony, L.; Chandran, Manoj; Rajmohan, K.

doi:10.17660/actahortic.2006.725.30

Cited by 14 publications

(7 citation statements)

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“…In the present investigation, interspecific crosses involving A. esculentus and wild relatives, followed by polyploidization, generated considerable variability in the amphidiploid derivatives. These results reaffirmed the earlier reports on the successful interspecific hybridization to generate considerable variability (Reddy, 2010;Arunkumar, 2015;Patel et al, 2021;Singh et al, 2023b) and the existence of barriers to the transmission of gene flow from wild species to the cultivated ones (Rajamony et al, 2006;Jatkar et al, 2007;. Widely variable amphidiploids have also been generated from the hybrids of wheat Frontiers in Plant Science frontiersin.org and related species of the genera Aegilops, Secale, and Thinopyrum by Nemeth et al (2015).…”

Section: Discussionsupporting

confidence: 85%

Trends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Discussionsupporting

confidence: 85%

Trends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding

2024

Frontiers Research Topics

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“…Successful interspecific hybridization in the species A. esculentus has helped to generate considerable variability [27]. However, barriers in the interspecific hybrids of okra have impeded the transmission of desired genes from wild species to the cultivated ones [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…The genetic distance and polyploidy nature of A. esculentus and other wild relatives are barriers to success in a hybridization programme to transfer desirable resistant genes [11]. However, the desired gene transfer from the wild to cultivated species has been hampered by barriers in the interspecific hybrids of okra [28,29]. Resistance to YVMV is not stable in developed varieties, and a frequent breakdown of resistance has been observed in cultivated species.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Okra Species, Their Hybrids and Crossability Relationships among Abelmoschus Species of the Western Ghats Region

et al. 2022

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Wild relatives of okra represent a good source of variation for breeding programs, particularly for traits related to biotic and abiotic stresses and fruit quality. However, wild species remain largely unexploited for okra breeding. The cultivated okra accession Abelmoschus esculentus cv. ‘Arka Anamika’ was crossed with three wild accessions: Abelmoschus manihot, Abelmoschus ficulens and Abelmoschus moschatus. The crossability was estimated based on fruit set, seed set and germination rate. The results of cross compatibility studies revealed that the crosses are compatible only when cultivated A. esculentus is used as a female, and reciprocal crosses were not successful in any of the three wild species. Higher fruit set (87.2%) and seed germination (92%) were obtained from the crosses ‘Arka Anamika’ × Abelmoschus manihot. The interspecific F1s exhibited normal growth, flowering and fruit formation, but the hybrids were completely sterile on selfing due to abnormal meiosis. Characterization of the parents and interspecific hybrids revealed that the interspecific hybrids were generally more vigorous than the parents and displayed greater alliance towards their wild parent. The sterile F1s were further treated with 0.1% colchicine to restore fertility. The three interspecific crosses showed considerable differences in mean performance and heterosis over mid parent and better parent. The cross ‘A. Anamika’ × A. manihot displayed positive heterosis over mid parent and better parent for the yield traits, viz., fruit yield per plant, number of fruits per plant, number of primary branches per plant and plant height. The information obtained in the study on crossability relationship, phenotypic characterization and heterosis in interspecific hybrids will assist breeders in the development of prebreeding material.

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“…Classical and mutation breeding programs aimed at resistance to diseases have rarely succeeded in okra (Rajamony et al 2006) [16] . Even though relative intervarietal variation in resistance has been observed in okra against shoot and fruit borer, none of the varieties showed complete resistance in these studies (Memon et al 2004) [12] .…”

Section: Introductionmentioning

confidence: 99%

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2019

J Pharmacogn Phytochem

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In planta transformation is a novel approach to generate transgenic with a short period of time. Somaclonal variation is a major constraint in tissue cultured dependent genetic transformation in Bhendi (Abelmoschus esculentus (L.). Therefore, agrobacterium tumifaciens mediated in planta transformation protocols was standardized using sprouted seed explants of bhendi cv. MDU-1, an important vegetable. agrobacterium -mediated transformation was performed using LBA4404 strain harboring the binary vector pBinAR carrying cry3a gene under the control of CaMV35s promoter and npt II gene as a selectable marker. The transformation event consisted of sonicating the explants for 3 minutes, vacuum infiltration (750 mm of Hg) for 2 minutes in Agrobacterium (pBinAR-cry3a) and co-cultivation for 3 days in MS medium with acetosyringone (100µM) showed transformation efficiency of 12.5%. The presence and integration of npt II and cry3a transgenes into the bhendi genome was confirmed by polymerase chain reaction (PCR). Work is in progress to grow the T0 seeds for molecular characterization of the inserted transgene using southern and western blot methods.

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In Vitro Embryo Rescue of Interspecific Crosses for Transferring Virus Resistance in Okra (Abelmoschus Esculentus (L.) Moench)

Cited by 14 publications

References 0 publications

Trends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding

Trends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding

Characterization of Okra Species, Their Hybrids and Crossability Relationships among Abelmoschus Species of the Western Ghats Region

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