In vitro embryo production in the cow: An effective alternative to the conventional embryo production approach

Bousquet, D.; Twagiramungu, H.; Morin, Nathalie; Brisson, Chantal; Carboneau, G; Durocher, J.

doi:10.1016/s0093-691x(98)00231-3

Cited by 90 publications

(44 citation statements)

References 25 publications

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“…Gonadotrophins such as FSH either alone (Bousquet et al 1999, Goodhand et al 1999, Sirard et al 1999 or in combination with LH (Blondin et al 2002, see a review by Merton et al 2003) and pregnant mare serum gonadotrophin (Pieterse et al 1991) have been used to improve the outcome of OPU in adult donor cows. Oocytes from superovulated cows are developmentally more competent than their non-stimulated counterparts (Faber et al 2003, Merton et al 2003, and specific changes in superovulatory treatment can greatly increase developmental competence of the oocyte (Blondin et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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Section: Discussionmentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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“…The ovarian stimulation protocol, artificial stimulation, and uterine flushing were conducted as described by Bousquet et al (1999).…”

Section: Production Of In Vivo Blastocystsmentioning

confidence: 99%

Comprehensive cross production system assessment of the impact of in vitro microenvironment on the expression of messengers and long non-coding RNAs in the bovine blastocyst

Côté

Vigneault²,

Laflamme³

et al. 2011

Reproduction

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In vitro production (IVP) of cattle embryos over the past two decades has revealed several negative impacts that have been attributed to the artificial microenvironment. Studies on embryos produced in vitro clearly point to aberrant gene expression levels. So far, the causal association between phenotype and measured gene expression has not led to substantial improvement of IVP systems. The aim of this study was to generate a unique dataset composed of microarray-derived relative transcript abundance values for blastocysts produced in ten in vitro systems differing primarily in culture medium formulation. Between-group comparisons determine the level of overall similarity among systems relative to in vivo reference embryos. The use of the dataset to contrast all in vitro treatments with the in vivo blastocysts pointed to a single common gene network. The 'boutique' array contained a panel of novel uncharacterized transcripts that were variably expressed depending on the medium in which the blastocysts were produced. These novel transcripts were differentially expressed in blastocysts even as carryover from conditions encountered 7 days earlier during oocyte maturation. All of the selected novel candidates thus expressed were from intergenic regions. The function of this long non-coding RNA remains unknown but clearly points to an additional level of complexity in early embryo development.

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“…Con el fin de superar estas limitantes se desarrolló la aspiración folicular guiada por ultrasonido (AFGU), una técnica reportada por Pieterse et al (1988), que ha demostrado ser exitosa y reproducible para la obtención de oocitos a partir de vacas y novillas, y que acoplada a la producción in vitro de embriones (PIVE), es más eficiente que la superovulación y la transferencia de embriones convencional (Bousquet et al, 1999;Hashimoto,1999).…”

Section: ■ Introducciónunclassified

Evaluación de la estimulación ovárica y la calidad de oocitos bovinos obtenidos por aspiración folicular

Giraldo¹,

Ramírez²,

Oquendo³

et al. 2017

J. agric. anim. sci.

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Introducción. La aspiración folicular guiada por ultrasonido (AFGU) acoplada a la producción in vitro de embriones (PIVE), es una alternativa importante para el aprovechamiento de los recursos genéticos de hembras bovinas de alto potencial productivo. Sin embargo, la calidad reducida de los oocitos recuperados por esta técnica, es uno de los aspectos que más afecta la eficiencia de obtención de embriones. Materiales y Métodos. Esta investigación comparó el desarrollo folicular, y la calidad de los oocitos recuperados por AFGU, en hembras bovinas superestimuladas convencionalmente con FSH, o con FSH y hCG. Resultados. Se evidenció una mayor calidad en los oocitos recuperados de animales estimulados con FSH y hCG, respecto a aquellos estimulados solo con FSH. Conclusiones. La inclusión de hCG en un esquema para la superestimulación ovárica con FSH de hembras bovinas posteriormente sometidas a aspiración folicular, ejerce un efecto favorable sobre la calidad de oocitos recuperados.Palabras claves: estimulación ovárica, folículos ováricos, gonadotropinas. Introduction. Ultrasound-guided follicular aspiration (UGFA) coupled with the in-vitro production of embryos is an important alternative for the use of genetic resources of bovine females of high productive potential. However, the reduced quality of oocytes recovered with this technique is one of the aspects with the highest impact on the efficiency to obtain embryos. Materials and methods. This research compared follicular development with the quality of oocytes recovered by UGFA, in bovine females conventionally over-stimulated with FSH or FSH and hCG. Results. A higher quality of oocytes recovered from animals stimulated with FSH and hCG was seen compared to the ones stimulated with FSH only. Conclusions. Inclusion of Hcg in a scheme for the ovarian over-stimulation with FSH of bovine females subject to follicular aspiration becomes a favorable effect on the quality of recovered oocytes.

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In vitro embryo production in the cow: An effective alternative to the conventional embryo production approach

Cited by 90 publications

References 25 publications

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Comprehensive cross production system assessment of the impact of in vitro microenvironment on the expression of messengers and long non-coding RNAs in the bovine blastocyst

Evaluación de la estimulación ovárica y la calidad de oocitos bovinos obtenidos por aspiración folicular

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