In vitro Efficacy Testing of Some Commercial Disinfectants against Pathogenic Bacteria Isolated from Different Poultry Farms

Abstract

Proper management and hygiene are the keys for the poultry industry, profit mainly depends on efficient cleaning and disinfection. Disinfectants play an essential role in controlling pathogens in health care, animal production, and food-related industries. The effectiveness of a disinfectant is mainly dependent on the active compound chosen, its concentration, and the cleanliness of the surfaces to which it is applied. The purpose of this study was to evaluate the effectiveness of some disinfectants (Prophyl 2000®, G7®, Pron-Tech®, Alkadox®, and Biodine®) at different concentrations and contact times at 20, 40, 60, and 90 minutes against field isolated serotypes of E. coli, Pasteurella multocida, Campylobacter jejuni, and Staphylococcus aureus from chicken and duck farms production at a titer of 3×10 6 CFU (Colony Forming Unit) /cm 2 in the absence and presence of organic matter (O.M). The results showed that the efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000® was the most powerful disinfectant against field pathogens, followed by G7®, Pron-Tech®, and Alkadox®, while Bio-dine® was the weakest disinfectant at the same conditions. Moreover, the organic matter hindered bactericidal power of many commercial disinfectants such as quaternary ammonium compounds (QUATS), halogen releasing agents including iodine, chlorine and its compounds, but some were not affected, such as glutaraldehyde. Finally, the success of the disinfection process in different poultry farms mainly depends on the selection of suitable disinfectants.

The prevalence of Salmonella species as a biosecurity indicator in poultry farms in Sharkia governorate, Egypt

“…The efficacy of Virocid (Glutaraldehyde, 0.5%), Virokin S (Potassium peroxymonosulfate and Sodium chloride 0.5 %) and Cid 2000 (Hydrogen peroxide and acetic acid, 2%) was assessed by applying each of them singly on S. Typhimurium isolates in the absence and presence of organic matter as previously described [19,20]. To test the disinfectant's effectiveness in the presence of organic matter, 9.5 mL of brain heart infusion broth supplemented with 2% yeast extract was inoculated with 0.5 mL of S. Typhimurium strain.…”