In Vitro Effects of Various Probiotic Products on Growth and Biofilm Formation of Clinical UPEC Strains

Abstract

<jats:title>Abstract</jats:title><jats:p>Uropathogenic <jats:italic>Escherichia coli</jats:italic> (UPEC) is the most prevalent pathogen causing urinary tract infections (UTIs). UPECs have various virulence factors such as adhesins, biofilm forming and toxin producing etc., to survive in urinary tract. Under certain circumstances probiotics are preferred for prevention and treatment of UTIs. In this study, we aimed to investigate the <jats:italic>in-vitro</jats:italic> effects of four different <jats:italic>Lactobacillus</jats:italic> spp. cell-free supernatants on growth and biofilm formation inhibition in clinically isolated UPEC strains. Growths of 50 UPEC strains were determined in 96-well microplate and measured in a spectrophotometer after four hours incubation at 37°C. Biofilm formation was detected by crystal violet staining method on three UPEC strains. Statistical analysis of growth and biofilm formation experiments were performed by Kruskal-Wallis and one-way ANOVA Tukey’s multiple-comparison tests, respectively. All tested cell-free supernatans of lactobacilli inhibited growths (<jats:italic>p</jats:italic>&lt;0. 0001) and biofilm formation (<jats:italic>p</jats:italic>&lt;0.05) of UPECs. All results were found to be statistically significant. As a conclusion, our findings supported previous studies which reported the high efficiency of these four <jats:italic>Lactobacillus</jats:italic> spp. in the prevention of UTIs.</jats:p>

Laktobasillerden elde edilen hücresiz süzüntülerin metisiline dirençli Staphylococcus aureus, vankomisine dirençli Enterococcus ve karbapeneme dirençli Klebsiella suşlarını inhibisyonu

“…Consistent with other studies, our results have shown that the inhibitory effects of CFSs of lactobacilli were found to vary depending on lactobacilli species, pathogens and concentration (11,42).…”

“…Inconsistent with Naderi, Saud et al (2020) detected that CFSs of lactobacilli strains isolated from milk, were shown to have no inhibitory effects on multi-drug resistant Klebsiella and Shigella species; however it was shown that S. aureus, E. coli, Salmonella Paratyphi, Salmonella Typhi, Pseudomonas, Proteus, Acinetobacter strains were inhibited in the presence of CFSs (45). Gumus et al (2020) have shown that the growth and biofilm formation of clinically isolated Uropathogenic E.coli (UPECs) were inhibited in the presence of L. rhamnosus, L. fermentum, L. acidophilus and L. plantarum CFSs (42). In the present study, we found that all CFSs and all concentrations have inhibitory effect on CRK.…”

The Influences of Lactobacillus Cell-Free Supernatants on Growth and Virulence Properties of Campylobacter Jejuni in Human Adenocarcinoma (Ht-29) Cell Culture

“…De-Man Rogosa-Sharpe (MRS) broth (Conda, Spain) was used on growth of Lactobacilli under anaerobic conditions at 37°C for 24 hours. Following overnight cultivation of Lactobacillus strains, supernatants were collected via centrifugation at 4000 rpm for 30 minutes at 4°C, then filtered with 0.2 μm pore size filters (12,23).…”

“…Therefore, alternative and supportive options need to be considered. There are studies that present promising results to cope with stages of infection using the antagonist relationship between probiotic microorganisms and pathogens (12)(13)(14)(15)(16)(17)(18)(19)(20).…”

The Power of Probiotics to Combat Urinary Tract Infections: A Comprehensive Review

“…Several studies have indicated that probiotics can interfere with the adhesion of uropathogenic E. coli (UPEC) to uroepithelial cells, which is a critical step in the development of UTIs 22 . Probiotics can also prevent the formation of biofilms by UPEC, which can increase their resistance to antibiotics and immune defenses 23 . By inhibiting the adhesion and colonization of uropathogens, probiotics can reduce the risk of UTIs 22,23 .…”