In vitro effects of tyre debris organic extract on the kinetic and morphologic traits of rabbit spermatozoa

Moretti, Elena; Bosco, Alessandro Dal; Mourvaki, Evangelia; Cardinali, R.; Collodel, Giulia; Geminiani, Michela; Cetta, Francesco; Giannerini, V.; Castellini, Cesare

doi:10.4995/wrs.2009.646

Cited by 2 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mean and standard deviation in the percentage of the most frequent alterations (acrosome, chromatin, axoneme, plasma membrane) observed by TEM in human sperm treated with 75 μg/ml and controls. toxicity [17,18]; considering that spermatozoa are highly differentiated and partially inactive cells from the transcriptional point of view, the incubation times are different from those used for other cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Tire Debris Organic Extracts on Human Spermatozoa

et al. 2011

View full text Add to dashboard Cite

Background & objectives:Environmental pollutants have detrimental effects on the reproductive function of human being and of animals. This study was aimed at evaluating the effects of tire debris, a fraction of particulate matter derived from tire wear, on motility and morphology of human spermatozoa.Methods: spermatozoa from ten healthy men were incubated for 4 hours with 10, 50 and 75 μg/ml of tire debris organic extract (TDOE). Sperm motility and morphology were evaluated according to WHO guidelines; the presence of apoptosis and necrosis was assessed by the AnnexinV/Propidium iodide assay. Ultrastructure of sperm organelles was observed by transmission electron microscopy.Results: incubation with 10 g/ml of TDOE did not affect sperm quality, except for the value of apoptosis (median: 8% vs. 6%; P<0.01). Sperm motility and sperm morphology percentages (P<0.01)decreased when sperm were treated with 50 and 75 μg/ml TDOE and the percentage of apoptotic and necrotic sperm (P<0.01) increased concomitantly. At the same concentrations TEM analysis showed an increased percentage of reacted and swollen acrosomes, disrupted chromatin and altered axonemes.Interpretation & conclusions: the organic part of tire debris is potentially harmful to spermatozoa, particularly at a concentration of 50-75 μg/ml, inhibiting motility and compromising morphological integrity. Nevertheless, these results cannot yet be extrapolated to the in vivo outcome due to the lack of studies focused on this issue.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Tire Debris Organic Extracts on Human Spermatozoa

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The range of concentrations was selected considering the data reported by Gualtieri et al [15] while the time of exposure was determined by Moretti et al [16]. Aliquots of samples not incubated with PM10 were used as controls.…”

Section: Sperm Selection and Pm10 Treatmentmentioning

confidence: 99%

“…The detection of apoptosis (phosphatidylserine externalization) and necrosis (disrupted membranes) in treated and untreated semen samples was performed with a Vybrant Apoptosis Assay kit (Invitrogen Ldt, UK) made up of Annexin V-FITC and Propidium iodide as reported in Moretti et al [16]. Observations were made and photographs were taken with a Leitz Aristoplan (Leica, Wetzlar, Germany) light microscope equipped with a fluorescence apparatus.…”

Section: Annexinv/propidium Iodide Assaymentioning

confidence: 99%

Effect of PM10 on Human Spermatozoa: An In Vitro Study

et al. 2011

View full text Add to dashboard Cite

This study was aimed at evaluating the in vitro effects of particulate matter (ranging from 2.5-10 m in an aerodynamic diameter, PM10) from an urban area on the motility of human spermatozoa.Samples of swim up selected human spermatozoa from three healthy men with normal semen parameters, according to WHO guidelines, were incubated for 4 hours with 10, 50 and 75 μg/ml of PM10. Kinetic parameters were evaluated with Computer-Assisted Sperm Analysis (CASA) and the presence of apoptosis and necrosis was assessed by AnnexinV/Propidium iodide assay.Data was statistically elaborated.Incubation of sperm with 10 g/ml PM10 for 4 hours did not affect sperm motility. On the contrary, a decrease in progressive motility was evident at 50 g/ml and 75 g/ml PM10. Among the kinetic parameters assessed with CASA system, the linearity variable was significantly lower in sperm treated with 50 g/ml and 75 g/ml PM10 vs controls (P<0.0001) and the amplitude of the lateral head displacement variable was significantly higher in sperm treated with 50 g/ml vs controls (P=0.0211). A substantial increase in necrosis, in up to 30% of sperm, was evident only in samples treated with 75 g/ml PM10.In conclusion, this preliminary study could indicate that PM10 is not potentially harmful to spermatozoa in vitro, except above a very high threshold (50-75 μg/ml) that is difficult to reach in vivo and that motility and viability are only partially affected.

show abstract

In vitro effects of tyre debris organic extract on the kinetic and morphologic traits of rabbit spermatozoa

Cited by 2 publications

References 17 publications

In Vitro Effects of Tire Debris Organic Extracts on Human Spermatozoa

In Vitro Effects of Tire Debris Organic Extracts on Human Spermatozoa

Effect of PM10 on Human Spermatozoa: An In Vitro Study

Contact Info

Product

Resources

About