In vitro effects of suspensions of selected nanoparticles (C60 fullerene, TiO2, SiO2) on Mytilus hemocytes

Canesi, Laura; Ciacci, Caterina; Vallotto, Davide; Gallo, Gabriella; Marcomini, Antonio; Pojana, Giulio

doi:10.1016/j.aquatox.2009.10.017

Cited by 206 publications

(114 citation statements)

References 53 publications

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“…As a consequence they are particularly susceptible to ENP exposure (Canesi et al, 2012). Previous in vivo and in vitro studies have reported adverse effects of TiO2NP on immune system and digestive gland function of M. galloprovincialis (Barmo et al, 2013;Canesi et al, 2010). In the present study we examined oxidative stress responses and genotoxic effects of TiO2NP and B(a)P, a carcinogenic PAH, in single and mixed exposures of these contaminants in the blue mussel M. edulis.…”

Section: Introductionmentioning

confidence: 94%

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Farkas

Bergum

Nilsen

et al. 2015

Science of The Total Environment

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Nanoparticles are emerging contaminants of concern. Knowledge on their environmental impacts is scarce, especially on their interactive effects with other contaminants. In this study we investigated effects of titanium dioxide nanoparticles (TiO2NP) on the blue mussel (Mytilus edulis) and determined their influence on the bioavailability and toxicity of benzo(a)pyrene (B(a)P), a carcinogenic polyaromatic hydrocarbon (PAH). Blue mussels were exposed to either TiO2NP (0.2 and 2.0 mg L -1 ) or B(a)P (20 µg L -1 ) and to the respective combinations of these two compounds. Aqueous contaminant concentrations, the uptake of Ti and B(a)P into mussel soft tissue, effects on oxidative stress and chromosomal damage were analyzed. The uncoated TiO2NP agglomerated rapidly in the seawater. The presence of TiO2NP significantly reduced the bioavailability of B(a)P, shown by lowered B(a)P concentrations in exposure tanks and in mussel tissue. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were impacted by the various exposures regimes, indicating oxidative stress in the contaminant exposure groups. While SOD activity was increased only in the 0.2TiO2NP exposure group, CAT activity was enhanced in both combined exposure groups.The GPx activity was increased only in the groups exposed to the two single compounds. In hemocytes, increased chromosomal damage was detected in mussels exposed to the single compounds, which was further increased after exposure to the combination of compounds. In this study we show that the presence of TiO2NP in the exposure system reduced B(a)P uptake in blue mussels. However, since most biomarker responses did not decrease despite lower B(a)P uptake in combined exposures, the results suggest that TiO2NP can act as additional stressor, or potentially alters B(a)P toxicity by activation.

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Section: Introductionmentioning

confidence: 94%

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Farkas

Bergum

Nilsen

et al. 2015

Science of The Total Environment

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“…In these studies, the particles are typically found to be agglomerated/aggregated, for example, TiO 2 [53,62,65,66], ZnO [43,53,[66][67][68], Ag [23,69], SiO 2 [70], and CeO 2 [53]. Manzo et al [71] analyzed an ZnO NP-contaminated soil by the Brunauer-Emmett-Teller method and found that the particles were not aggregated.…”

Section: Soil Propertiesmentioning

confidence: 99%

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

Tourinho

Gestel²,

Lofts

et al. 2012

Enviro Toxic and Chemistry

396

229

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Abstract-Metal-based nanoparticles (NPs) (e.g., silver, zinc oxide, titanium dioxide, iron oxide) are being widely used in the nanotechnology industry. Because of the release of particles from NP-containing products, it is likely that NPs will enter the soil compartment, especially through land application of sewage sludge derived from wastewater treatment. This review presents an overview of the literature dealing with the fate and effects of metal-based NPs in soil. In the environment, the characteristics of NPs (e.g., size, shape, surface charge) and soil (e.g., pH, ionic strength, organic matter, and clay content) will affect physical and chemical processes, resulting in NP dissolution, agglomeration, and aggregation. The behavior of NPs in soil will control their mobility and their bioavailability to soil organisms. Consequently, exposure characterization in ecotoxicological studies should obtain as much information as possible about dissolution, agglomeration, and aggregation processes. Comparing existing studies is a challenging task, because no standards exist for toxicity tests with NPs. In many cases, the reporting of associated characterization data is sparse, or missing, making it impossible to interpret and explain observed differences in results among studies. Environ. Toxicol. Chem.

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“…C 60 fullerenes administration increased the hepatic 8-oxodG level and high dose generated 8-oxodG in the lung [206]. In oysters they are known for affecting oyster embryonic development, cellular damage in the alimentary canal in Daphnia magna, growth inhibition in freshwater fish Carassius auratus, increase in mortality rates in gestating daphnids, nitric oxide production in Mytilus hemocytes [211][212][213][214][215].…”

Section: Mechanistic Studiesmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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In vitro effects of suspensions of selected nanoparticles (C60 fullerene, TiO2, SiO2) on Mytilus hemocytes

Cited by 206 publications

References 53 publications

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

In vitro and in vivo toxicity assessment of nanoparticles

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