In Vitro Effects of Some Synthesized Aminoacetanilide N’-Substituted on Human Leukocytes Separated From Peripheral Blood

Abstract

Local anaesthetics are frequently used in several medical areas like surgical interventions, stomatology or preparing the patient for imagistic techniques like endoscopies. In most cases, the local anaesthetics are administrated in inflammatory processes and several studies demonstrated that these drugs may influence the response of local immune system cells. The adverse effects reported are correlated with the route of administration and the interaction with cellular components at the site of administration. Some lidocaine analogues with aminoacetanilide N'-substituted structure were previously synthesized, characterized and pharmacologically tested in preclinical studies regarding the local anaesthetic effects and were selected for the present research. The study aims to determine the effects of the synthetized aminoacetanilide N'-substituted on the release of oxygen metabolites by polymorfonuclear cells and on the cellular viability of human leukocytes isolated from the peripheral blood. Rezumat Anestezicele locale sunt utilizate frecvent în multe domenii medicale, cum ar fi intervențiile chirurgicale, stomatologia sau pregătirea pacientului pentru tehnici imagistice, de exemplu endoscopiile. În majoritatea cazurilor, anestezicele locale sunt administrate în procese inflamatorii și mai multe studii au demonstrat că aceste medicamente pot influența răspunsul celulelor sistemului imunitar local. Efectele adverse raportate sunt corelate cu calea de administrare și interacțiunea cu componentele celulare la locul de administrare. Anumiți analogi de lidocaină cu structura aminoacetanilidă N'-substituită au fost anterior sintetizați, caracterizați și testați farmacologic în studii preclinice, privind efectele anestezice locale și au fost selectați pentru prezenta cercetare. Studiul își propune să determine efectele aminoacetanilidei N'-substituite asupra eliberarării metaboliților oxigenului de către celulele polimorfonucleare și asupra viabilității celulare a leucocitelor umane izolate din sângele periferic.

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“…The abundance of essential amino acids, vitamins, and minerals makes mussel meat a valuable functional food for strengthening the immune system. As a result, nutritionists recommend the inclusion of seafood in general in diets to enhance immunity, especially in the current epidemiological context coupled with the increase in global pollution and even many drugs that affect the immune system [ 12 , 13 , 14 ].…”

Food Habits and Lifestyle of Romanians in the Context of the COVID-19 Pandemic

“…Independent variables are: Gender where the reference group is “female”; BMI group where the reference group is “normal weight”; Age group where the reference group is young people in the range “[ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]”. …”

“…Age group where the reference group is young people in the range “[ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]”.…”

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