In vitro effects of Sho-saiko-to on production of granulocyte colony-stimulating factor by mononuclear cells from patients with chronic hepatitis C

Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

“…Xiao-chai-hu-tang (XCHT) has been used as a major curing prescription for chronic hepatitis in the traditional Chinese herbal medicine (Yamashiki et al, 1997;Yagura et al, 2001). Baicalein (a flavonoid compound) and baicalin (glycoside of baicalein) are reported to be the major bioactive components in the XCHT (Inoue and Jackson, 1999;Ueda et al, 2001).…”

Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine

“…flavonoids, saponins and its mixture (3) 35% MeOH solution of the flavonoids-saponins mixture was applied to the ODS column that had been rinsed with 35% MeOH (4) Gradient RP-HPLC from 35% MeOH to MeOH, to obtain two fractions, flavonoids and saponins (5) Each saponins fraction and flavonoids fraction is collected to dryness (6) M-saponins were extracted with diethyl ether under sonication (7) The extract was filtered and concentrated to afford M-phenylalkanoid fractions Chemicals Co. Ltd., Tokyo, Japan) column chromatography with aqueous-methanol gradient eluting. The eluted fraction were collected, pooled, and then checked by RP HPLC analysis using a PDA detector.…”

“…The flow rate of the mobile phase and the column temperature were 1.0 ml/min or 40 • C, respectively (5) The UV data of the effluent from the column ranging from 200 to 400 nm were collected, and the peak analysis and assignment were performed with the system analysis software (CLASS-LC10, Shimadzu, Kyoto, Japan) This is a partial modification of the method reported by Nakai et al [136]. Table 6.…”

“…Phytochemical analysis of SST and modern scientific studies on its pharmacological actions were commenced about 30 years ago. Several clinical studies have demonstrated the therapeutic efficacy of SST in patients with chronic viral hepatitis [4,5]. Many experimental reports have demonstrated the pharmacological actions of SST.…”

Xiao Chai Hu Tang inhibits CVB1 virus infection of CCFS-1 cells through the induction of Type I interferon expression

“…Studies have shown that XCHT possessed antioxidant [20,21], immunomodulating [15,[22][23][24] and antiproliferative [25,26] activities. It ameliorates chronic viral infected liver diseases [25,26], enhances production of IFN [15,27,28] and antibodies in patients with chronic hepatitis [29].…”