In vitro effects of Moringa oleifera seed lectins on Haemonchus contortus in larval and adult stages

Medeiros, Mário Luan Silva de; Alves, Robson R.V.; Oliveira, Benny Ferreira de; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes; Bezerra, Ana Carla Diógenes Suassuna; Silva, Michele D.C.

doi:10.1016/j.exppara.2020.108004

Cited by 9 publications

(9 citation statements)

References 46 publications

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“…The differences in concentration used in the two studies and the difference in plant material are thought to be responsible for the variation in motility inhibition rates observed. According to previous studies, Medeiros et al [29] detect in their study a higher proteolytic activity in extracts of adult male and female worms after incubation with lectins. In these male and female worms, changes in cuticle ridges, longitudinal striations, and vulva marked lectin-induced morphological changes Medeiros et al [27].…”

Section: Discussionmentioning

confidence: 72%

“…The inhibition was total (100%) after 6h of exposure to the concentrations of 1200 and 2400 µg/mL of the two extracts of the two plants. Weaker results (63%) were obtained in vitro by [29] with Moringa oleifera proteins on the motility of adult male and female worms of H. contortus. The differences in concentration used in the two studies and the difference in plant material are thought to be responsible for the variation in motility inhibition rates observed.…”

Section: Discussionmentioning

confidence: 85%

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In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants

Kuiseu¹,

Olounladé²,

Houssoukpe³

et al. 2022

AVS

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This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p < 0.001) at the highest concentrations (1200 and 2400 µg/mL) than at the lowest. The hydro ethanolic extract of A. leiocarpus showed the highest inhibition (R 2 = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R 2 = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (p<0.05); and also dependent on incubation time (p<0.01). At 2400 µg/mL, all adult worms were immobile after 6 h of exposure, whereas at the lowest concentration (75 µg/mL), all adult worms were immobile after 36 h of exposure. Extracts from both plants had similar activity. Finally, the results of the present study suggest that the both plants studied have anthelmintic properties in vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 85%

In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants

Kuiseu¹,

Olounladé²,

Houssoukpe³

et al. 2022

AVS

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“…The entire filtrate was evaporated using a vacuum evaporator to obtain a concentrated extract, followed by freeze-drying at -50°C for 30 hours to produce a dry ethanolic extract of C. vieillardii. The dried macroalgae extract samples were dissolved in an in vitro test medium consisting of 0.9% sodium chloride solution (Medeiros et al, 2020) to obtain concentrations of 2 mg/ mL, 4 mg/mL, and 6 mg/mL, following the preparation methods of Alvarez-Mercado et al (2015), Sakti et al (2018), and Bonde et al (2021).…”

Section: Extraction Processmentioning

confidence: 99%

In vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep

Sakti,

Suwignyo,

Sofyan

et al. 2024

VIS

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This study aimed to evaluate the potential of macroalgae Chaetomorpha vieillardii as an in vitro candidate for bio-anthelmintic. Adult Worm Motility Test (AWMT) was conducted at concentration levels of 2 mg/mL, 4 mg/mL, and 6 mg/mL of C. vieillardii ethanol extract on 150 female H. contortus worms, randomly divided into 6 treatments with 5 replications, using a two way completely randomized factorial design. 0.9% sodium chloride was used as a negative control, and albendazole at 0.5 and 2 mg/mL served as positive controls. Worm motility observations were performed at 0, 0.25, 0.5, 1, 2, and subsequent time intervals until 100% of the worms were deceased. The identical treatment was applied to the egg hatching inhibiting test (EHIT) using a completely randomized design in a one-way pattern by observing the number of eggs at 0 and 24 hours. The results showed that the concentration level of C. vieillardii ethanol extract and the observation time significantly influenced (P<0.01) the motility of H. contortus, and there was an interaction between them (P<0.01). All three levels were able to decrease the motility of H. contortus, but they were unable to match albendazole. Despite of this result, the three levels were equally effective (P<0.01) as albendazole in terms of inhibiting the egg-hatching of H. contortus. The 2 mg/mL level was sufficient to inhibit the motility and egg-hatching of H. contortus. These findings indicated that the ethanol extract of C. vieillardii has the potential to be developed as a bio-anthelmintic for ruminants.

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“…One of the coagulant molecules of M. oleifera seeds is a cationic lectin (cMoL), specific for galactose, that has been shown to be resistant to a wide pH range and high temperatures. The partially characterized lectin was revealed to have a molecular profile of 26.5 KDa, an approximate isoelectric point of 11.67, 101 amino acids [22] and the following biological activities: anticoagulant properties [23], insecticidal properties [24], antiparasitic properties [25], cytotoxicity to tumor cells [26] and a clarifying ability of water turbidity similar to aluminum sulfate treatment [22].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization Using Biosensors with the Coagulant Moringa oleifera Seed Lectin (cMoL)

et al. 2023

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Triturated Moringa oleifera seeds have components that adsorb recalcitrant indigo carmine dye. Coagulating proteins known as lectins (carbohydrate-binding proteins) have already been purified from the powder of these seeds, in milligram amounts. The coagulant lectin from M. oleifera seeds (cMoL) was characterized by potentiometry and scanning electron microscopy (SEM) using MOFs, or metal–organic frameworks, of [Cu3(BTC)2(H2O)3]n to immobilize cMoL and construct biosensors. The potentiometric biosensor revealed an increase in the electrochemical potential resulting from the Pt/MOF/cMoL interaction with different concentrations of galactose in the electrolytic medium. The developed aluminum batteries constructed with recycled cans degraded an indigo carmine dye solution; the oxide reduction reactions of the batteries generated Al(OH)3, promoting dye electrocoagulation. Biosensors were used to investigate cMoL interactions with a specific galactose concentration and monitored residual dye. SEM revealed the components of the electrode assembly steps. Cyclic voltammetry showed differentiated redox peaks related to dye residue quantification by cMoL. Electrochemical systems were used to evaluate cMoL interactions with galactose ligands and efficiently degraded dye. Biosensors could be used for lectin characterization and monitoring dye residues in environmental effluents of the textile industry.

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In vitro effects of Moringa oleifera seed lectins on Haemonchus contortus in larval and adult stages

Cited by 9 publications

References 46 publications

<i>In vitro</i> Effects of <i>Anogeissus leiocarpus</i> and <i>Adansonia digitata</i> on Two Life-cycle Stages of <i>Haemonchus contortus</i>, a Gastrointestinal Parasite of Small Ruminants

<i>In vitro</i> Effects of <i>Anogeissus leiocarpus</i> and <i>Adansonia digitata</i> on Two Life-cycle Stages of <i>Haemonchus contortus</i>, a Gastrointestinal Parasite of Small Ruminants

In vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep

Electrochemical Characterization Using Biosensors with the Coagulant Moringa oleifera Seed Lectin (cMoL)

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In vitro effects of Moringa oleifera seed lectins on Haemonchus contortus in larval and adult stages

Cited by 9 publications

References 46 publications

&lt;i&gt;In vitro&lt;/i&gt; Effects of &lt;i&gt;Anogeissus leiocarpus&lt;/i&gt; and &lt;i&gt;Adansonia digitata&lt;/i&gt; on Two Life-cycle Stages of &lt;i&gt;Haemonchus contortus&lt;/i&gt;, a Gastrointestinal Parasite of Small Ruminants

&lt;i&gt;In vitro&lt;/i&gt; Effects of &lt;i&gt;Anogeissus leiocarpus&lt;/i&gt; and &lt;i&gt;Adansonia digitata&lt;/i&gt; on Two Life-cycle Stages of &lt;i&gt;Haemonchus contortus&lt;/i&gt;, a Gastrointestinal Parasite of Small Ruminants

In vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep

Electrochemical Characterization Using Biosensors with the Coagulant Moringa oleifera Seed Lectin (cMoL)

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<i>In vitro</i> Effects of <i>Anogeissus leiocarpus</i> and <i>Adansonia digitata</i> on Two Life-cycle Stages of <i>Haemonchus contortus</i>, a Gastrointestinal Parasite of Small Ruminants

<i>In vitro</i> Effects of <i>Anogeissus leiocarpus</i> and <i>Adansonia digitata</i> on Two Life-cycle Stages of <i>Haemonchus contortus</i>, a Gastrointestinal Parasite of Small Ruminants