In vitro effects of LPS, IL-2, PDGF and CRF on haemocytes of Mytilus galloprovincialis Lmk.

Specific expression of antimicrobial peptide and HSP70 genes in response to heat-shock and several bacterial challenges in mussels

“…Surface properties are crucial in influencing Escherichia coli persistence and survival within M. galloprovincialis hemolymph [33]. In M. galloprovincialis, in vitro addition of E. coli O26:B6 LPS to hemocytes resulted in over-expression of a membrane molecule related to IL-2Ra and in stimulation of release of dopamine, adrenaline and noradrenaline [34]. In vitro antibacterial activity of M. edulis was provoked by E. coli LPS extract in a dose-dependent manner (0.1 versus 10 mg) and was more powerful at 20 C than at 6 C, suggesting involvement of hemocyte metabolism [35].…”

Molecular cloning and expression of a Toll receptor gene homologue from Zhikong Scallop, Chlamys farreri

“…Here, the mixed primary culture of haemocytes was prepared as described by Barcia [29,30] with some modification. The haemolymph was withdrawn by a sterile syringe from the adductor muscle and simultaneously diluted (1:3) in anticoagulant (modified Alsever's solution, 20.8 g L À1 glucose, 8 g L À1 sodium citrate, 3.36 g L À1 EDTA and 22.5 g L À1 NaCl, pH 7.0 and 1000 mOsm).…”

Molecular cloning and characterization of a putative lipopolysaccharide-induced TNF-α factor (LITAF) gene homologue from Zhikong scallop Chlamys farreri

“…The mixed primary cultured haemocytes were prepared as described by Barcia [27,28] with some modifications [29]. The haemolymph was withdrawn by a sterile syringe from the adductor muscle and simultaneously diluted (1:3) in anticoagulant (modified Alsever's solution, 20.8 g L À1 glucose, 8 g L À1 sodium citrate, 3.36 g L À1 EDTA and 22.5 g L À1 NaCl, pH 7.0 and 1000 mOsm).…”