In Vitro Effects of Human Prolactin and Oxytocin on Sulfatase Activity in Isolated Human Decidual Cells∗

Abstract

Homogeneous preparations of decidual cells were obtained from term decidual tissue adherent to fetal membranes by using a slightly modified version of a technique developed for the isolation of decidual cells from first and second trimester decidua. The effects of human PRL (hPRL) and oxytocin on the kinetics of the hydrolysis of estrone sulfate were determined in decidual cells prepared from tissue obtained before and after the onset of labor. In addition, sulfatase activity in decidual cells isolated from term decidua was compared with those of chorionic cells isolated from chorion leave of the same pregnancy. Chorionic cells had significantly higher (mean, 2.5-fold) levels of sulfatase activity than the corresponding decidual cells. The mean sulfatase activity in decidual cells obtained after normal vaginal delivery [25 +/- 19 (+/- SE) nmol/mg protein X 15 min) was higher than that in decidual cells obtained from patients undergoing cesarean section before the onset of labor (1.7 +/- 0.11). This difference was significant (P less than 0.02, by Mann-Whitney test) in spite of the large variation in activity in preparations from vaginal deliveries. hPRL (500 ng/ml) and oxytocin (0.2 microM) had similar effects on sulfatase activity in decidual cells in a manner dependent on whether the cells were isolated from tissue obtained before or after labor. In cells isolated from fetal membranes obtained before labor (cesarean delivery), hPRL or oxytocin significantly stimulated sulfatase activity, whereas in decidual cells obtained after vaginal delivery, both hPRL and oxytocin significantly inhibited sulfatase activity. The Michaelis constants for the hydrolysis of estrone sulfate (Km, 22 +/- 4.8 microM) were not affected by these hormones. Since the mean sulfatase activity of decidual cells obtained before labor was approximately 10-fold higher than the activity reported for endometrial stromal cells, PRL produced by decidual cells may act in vivo as an autocrine factor to stimulate their sulfatase activity.

Thrombin-enhanced matrix metalloproteinase-1 expression: a mechanism linking placental abruption with premature rupture of the membranes

“…Decidual cells were isolated from third-trimester placentas as described by Braverman and Gurpide 22 . Fetal membranes were obtained from non-laboring women undergoing repeat, term, Cesarean deliveries following uncomplicated pregnancies.…”

Placental Steroid Hormone Biosynthesis in Primate Pregnancy*

“…Because the K m value of sulfatase is much greater than that of 3/3-HSD and aromatase, it has been suggested by Gibb and Lavoie (253) that at low steroid concentrations the sulfatase would become ratelimiting in the synthesis of estrogen from DHAS, while only at micromolar concentrations of DHAS would aromatase become rate-limiting. Although the regulation of sulfatase is unknown, PRL and oxytocin stimulated enzyme activity in decidual cells isolated before the onset of human labor (254). Mitchell etal.…”